Portage Microbiology Exam #5 EXAM PRACTICE
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Define the concept of universal Universal precautions means any and all samples,
precautions (short answer) whether known or unknown, are to be treated as
potentially hazardous (or pathogenic) materials
List at lease 3 observations a Need to note:
researcher would be sure to note 1. Size and shape
while assessing and unknown 2. Any observable motility
microbial sample (short answer) 3. Gram status (positive or negative)
While observing an unknown sample You must determine motility before determining
of limited amounts, a researcher Gram status. Motility requires a wet mount, while
must determine the following gram staining requires heat fixing the sample. If
observations: 1) the presence of any one were to begin with the gram stain, the heat
motility and 2) its Gram status using fixation process would kill the organism, making
the same sample- the liquid sample any observations regarding motility impossible. The
cannot be divided. Which would you correct approach would be to place the liquid
determine first and why? (short culture on a glass slide and determine its motility
answer) status. Next, the same liquid culture can be heat
fixed and gram stained.
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