DNA possesses
a) a net negative charge
b) a net positive charge
c) no net charge
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a
Your initial DNA extract has a DNA concentration of 900 ng/uL. What volume of this
extract would you add to water to get 50 uL of 100 ng/uL DNA solution.
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, add 5.56 uL of extract to 44.44 uL of water
gives you the length of your consensus sequence
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trim consensus
true or false:
you should always be sure to put BOTH a forward AND reverse primer in EACH
sequencing tube.
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false
lab week 6-10
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extract dna, pcr, and barcode samples
are DNA strands parallel or antiparallel?
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antiparallel with the 3' end of one strand and the 5' end of the other at the
same end of the double stranded molecule.
The rate of migration of DNA within an agarose gel in the gel electrophoresis
technique is primarily based on what factor?
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The size of the DNA fragments
What does the 260/280 ratio reflect?
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this ratio tells you about protein contamination, no salt contamination, not
less.
shows you the most common nucleotide at each position in the sequence
Give this one a try later!
consensus
, After running your DNA extract through a nanodrop spectrophotometer you find that
your extract has a DNA concentration of 0.1 ng/uL and your A260:280 is 1.80, which of
the following should you do to increase the likelihood of your DNA extract PCR
amplifying correctly?
Give this one a try later!
You should do another extraction, your DNA concentration is too low
What are a necessary "ingredients" for a PCR to proceed
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Nucleotides, DNA polymerase (or Taq polymerase), Reverse Primer,
Template DNA, and Forward Primer
Which of the following are advantages of scholarly literature? (Choose all that apply)
a) It is reliable
b) It uses common vocabulary
c) It is detailed
d) It is transparent with sources of information
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a,c,d
5'-ATGCAATTGCAGTCGATTGCTCGATCGATCTTCGAAATTTCCCGAGCTTCGATCGC-
3'
a) a net negative charge
b) a net positive charge
c) no net charge
Give this one a try later!
a
Your initial DNA extract has a DNA concentration of 900 ng/uL. What volume of this
extract would you add to water to get 50 uL of 100 ng/uL DNA solution.
Give this one a try later!
, add 5.56 uL of extract to 44.44 uL of water
gives you the length of your consensus sequence
Give this one a try later!
trim consensus
true or false:
you should always be sure to put BOTH a forward AND reverse primer in EACH
sequencing tube.
Give this one a try later!
false
lab week 6-10
Give this one a try later!
extract dna, pcr, and barcode samples
are DNA strands parallel or antiparallel?
,Give this one a try later!
antiparallel with the 3' end of one strand and the 5' end of the other at the
same end of the double stranded molecule.
The rate of migration of DNA within an agarose gel in the gel electrophoresis
technique is primarily based on what factor?
Give this one a try later!
The size of the DNA fragments
What does the 260/280 ratio reflect?
Give this one a try later!
this ratio tells you about protein contamination, no salt contamination, not
less.
shows you the most common nucleotide at each position in the sequence
Give this one a try later!
consensus
, After running your DNA extract through a nanodrop spectrophotometer you find that
your extract has a DNA concentration of 0.1 ng/uL and your A260:280 is 1.80, which of
the following should you do to increase the likelihood of your DNA extract PCR
amplifying correctly?
Give this one a try later!
You should do another extraction, your DNA concentration is too low
What are a necessary "ingredients" for a PCR to proceed
Give this one a try later!
Nucleotides, DNA polymerase (or Taq polymerase), Reverse Primer,
Template DNA, and Forward Primer
Which of the following are advantages of scholarly literature? (Choose all that apply)
a) It is reliable
b) It uses common vocabulary
c) It is detailed
d) It is transparent with sources of information
Give this one a try later!
a,c,d
5'-ATGCAATTGCAGTCGATTGCTCGATCGATCTTCGAAATTTCCCGAGCTTCGATCGC-
3'
