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VERIFIED ANSWERS | LATEST UPDATE
Filtration - ANSWER -Traps microorganisms as fluid passes through a filter,
organisms above a certain size threshold are trapped in the pores
HEPA - ANSWER -High-efficiency particulate air filter
Ultraviolet Light - ANSWER -can be used to control microbial growth by causing
thymine molecules to link together
What is impaired when UV light is used - ANSWER -Errors in protein synthesis
and impairment of chromosome replication occur
X rays and Gamma rays (ionizing radiations) - ANSWER -force electrons out of
microbial molecules, affects cell metabolism and physiology
Sterilization - ANSWER -involves destruction of all living microbes, spores, and
viruses
Sanitation - ANSWER -reduces the numbers of pathogens or discourages their
growth
Antiseptics - ANSWER -are used to destroy pathogens on living tissue
Sanitizing - ANSWER -means reducing microbial population to a safe level
Sepsis - ANSWER -refers to contamination by microorganisms
Degerming - ANSWER -means removing organisms from an objects surface
, Antiseptics and Disinfectants have distinctive properties - ANSWER -Able to kill
o r slow growth of microbes, Nontoxic to humans and animals, Soluble in water,
Storable, substantial shelf life, Effective quickly and at low concentration, Not
corrosive and instruments, Does not combine with organic matter, Easy to obtain
and inexpensive
The phenol coefficient (PC) - ANSWER -indicates disinfecting ability compared
to that of phenol
An in-use test - ANSWER -to compare samples of substrate before and after
disinfection
2 Tests used to evaluate for antiseptic's effectiveness - ANSWER -phenol
coefficient and in-use test
Phenol and Phenolic Compounds - ANSWER -denature proteins
Negative points of Phenol: - ANSWER -Is expensive, Is caustic, Has a pungent
odor
Positive points of Derivatives - ANSWER -Have greater germicidal activity and
have lower toxicity than phenol
Bisphenols - ANSWER -are combinations of two phenol molecules
Halogens - ANSWER -oxidize proteins
Chlorine - ANSWER -keeps bacterial populations low in municipal water supplies
and swimming pools
Iodine - ANSWER -is used to disinfect wounds, water, and restaurant equipment
Iodophores - ANSWER -release iodine over a long period of time
Heavy metals - ANSWER -interfere with microbial metabolism
, Mercury, copper, and silver - ANSWER -are reactive with proteins, disrupting
cellular metabolism, killing microbes
Alcohols - ANSWER -denature proteins and disrupt membranes
Ethanol - ANSWER -is effective against vegetative cells but not spores
Membrane disruption is caused by - ANSWER -lipid dissolution
Soaps and Detergents act as - ANSWER -Surface-Active Agents
Soaps remove microbes by - ANSWER -emulsifying and solubilizing particles on
the skin
Detergents - ANSWER -Are surfactants and they also cause cytoplasm leakage
from microbial membranes
Quaternary ammonium compounds - ANSWER -react with cell membranes and
destroy some bacteria and viruses
Hydrogen Peroxide - ANSWER -Damages cellular components and contains
Catalase in wounded tissue that transforms it to oxygen and water, the oxygen is
effective against anaerobic bacteria
Some chemical Agents can be used for sterilization - ANSWER -Aldehydes,
Ethynlene oxide, and Cholorine dioxide
Aldehydes - ANSWER -cause cross-linking that inactivates proteins and nucleic
acids
Formaldehyde - ANSWER -20% in 70% alcohol for 18 hours or as a gas for 12
hours
2 types of Aldehydes - ANSWER -Formaldehyde and Glutaraldehyde