MCB: ALL EXAM QUESTIONS AND CORREC
50 cmultiple cchoice cquestions, ccovering call cthe ctopics cwe've ctaught cyou cover cthe ccourse cof cthe
csplit cinto c4 csections: c1) cProteins c(10 cpoints) c2) cDNA c(10 cpoints), c3) cTranscription c& cTranslat
cEnergy c& cMetabolism c(20 cpoints). cThe cbest cway cto cprepare cfor cthis cexam cis cto creview cyour
c"big cpictures" cof ceach clecture cusing cthe c"specifics cto cknow" cprovided. cYou ccan cexpect c1 cor c
cCORRECT cANS✔✔
Exam c1 cversion cA c- cCORRECT cANS✔✔protein cstructure cand cfunction
Many ctypes cof cnon-covalent cinteractions care celectrostatic cin cnature; cone cnotable cexception cis
A) cHydrogen cbond c
C) cIonic cinteraction
B) cVan cder cWaals cinteraction c
D) cHydrophobic cinteraction c- cCORRECT cANS✔✔D
2. cMaintaining ccorrect cpH cis cvery cimportant cfor cliving csystems. cWhich cof cthe cfollowing cmolecu
important cfor cpH ccontrol cin cliving csystems?
A) cProteins, cespecially cthose cwith chigh ccontent cof chistidine cresidues
B) cDihydrogen cphosphate cand chydrogen cphosphate
C) cBicarbonate
D) cAll cof cthe cabove c- cCORRECT cANS✔✔D
3. cWhile cmany cbiochemists cuse cthe cterms c"aspartate" cand c"aspartic cacid" cinterchangeably, cthe
different cthings. cWhich cof cthe cfollowing cregarding cthese ctwo cterms cis ctrue?
A) cAspartate cis cthe cconjugate cbase cof caspartic cacid.
B) cAspartate cis cconverted cto caspartic cacid cupon can cincrease cin cpH.
C) cAspartic cacid cis cconverted cto caspartate cupon ca cdecrease cin cpH.
D) cAll cof cthe cabove. c- cCORRECT cANS✔✔A
4. cMost clikely, cthe cdiagram con cthe cright cillustrates
A) cA chydrogen cbond cformed cbetween ca cbase-pair
B) cA chydrogen cbond cformed cbetween ctwo cadjacent cbeta-strands
C) cAn cionic cinteraction cbetween ctwo cadjacent calpha chelices
D) cA chydrogen cbond cformed cbetween ctwo cadjacent calpha chelices c- cCORRECT cANS✔✔pictur
B
,D) cHistidine; cproton cdonor c- cCORRECT cANS✔✔picture
B
8. cCystine cis cformed cvia
A) cReduction cof cdisulfide cbond
B) cOxidation cof cthe cside cchain cof ctwo ccysteines
C) cDimerization cbetween ca ccysteine cand ca ctyrosine
D) cDimerization cbetween ca ccysteine cand ca cmethionine c- cCORRECT cANS✔✔B
9. cWhich cof cthe cfollowing cstatements cis ctrue cregarding cthe ctwo
amino cacids cshown con cthe cright?
A) cBoth ccan cbe csynthesized cusing cpyruvate cas ca cdirect cprecursor
B) cBoth ccan cbe csynthesized cusing coxaloacetate cas ca cdirect cprecursor
C) cThe cone con cthe cleft cbut cnot cthe cone con cthe cright ccan cbe
synthesized cusing cpyruvate cas ca cdirect cprecursor
D) cBoth care cD-aspartate c- cCORRECT cANS✔✔picture
B
10. cHistidine cis can camino cacid cwith cthree ctitratable cgroups: can c−NH3+ cgroup c(pKa=9.2), ca c−C
can cimidazole c(amine-like) cgroup c(pKa=6.0). cThe cpI cof chistidine cis
A) c5.5 c
B) c7.6 c
C) c3.9 c
D) c8.5 c- cCORRECT cANS✔✔B
11. cAs can cinhibitor cfor cthe cshikimate cpathway, cglyphosate c(roundup) cis cable cto ckill cweeds cbec
A) cIt cblocks cthe csynthesis cof cbranched cchain camino cacids csuch cas cIle cand cVal
B) cIt cblocks cthe cconversion cof calpha-ketoglutarate cto cglutamate
C) cIt cblocks cthe csynthesis cof caromatic camino cacids csuch cas cPhe cand cTrp
D) cIt cstimulates chuman cbeings cto cpull cthe cweeds cout cof ctheir cyard c- cCORRECT cANS✔✔C
12. cWhich cof cthe cfollowing csets cof camino cacids chave cthree cionizable cgroups?
A) cAla, cPro, cAsn
B) cAsn, cAsp, cGln
C) cAsp, cSer, cTrp
D) cAsp, cCys, cTyr c- cCORRECT cANS✔✔D
picture cof cpetptide cchain cwith cside cchains: cCH3, cCH2(ring)OH, cCH2COO-, cH
13. cThe csequence cof cthis cpeptide cis
A) cAFDG cB) cAYDG cC) cAYEG cD) cGYEA
14. cThe cisoelectric cpoint c(pI) cof cthe cpeptide cis:
A) c3.6 cB) c6.1 cC) c6.5 cD) c7.0 c- cCORRECT cANS✔✔sequence:
pI:
13) cB
14)a
15. cWhich cof cthe cfollowing cresidues care crarely cfound cin can calpha chelix?
A) cGlu, cArg, cLys
B) cTyr, cPhe, cLeu
, experiment, ctwo cchemical creagents c- curea cand cbeta-mercaptoethanol c- cwere cused. cThe cfuncti
is cto c_________ cand cthe cfunction cof cbeta-mercaptoethanol cis cto c______________. cBetween ct
reagents, cthis cone c_____________ cshould cbe cremoved cfirst cto censure ccorrect crefolding cof cthe
A) cdenature cthe cprotein; coxidize cthe cprotein; cbeta-mercaptoethanol
B) cdenature cthe cprotein; creduce cthe cdisulfide cbonds; curea
C) creduce cthe cdisulfide cbonds; cdenature cthe cprotein; cbeta-mercaptoethanol
D) csolubilize cthe cprotein; creduce cthe cdisulfide cbonds; curea c- cCORRECT cANS✔✔B
19. cWhich cof cthe cfollowing ccorrectly cdescribes cthe corder cof cevents cin cdetermining cprotein cstru
X-ray ccrystallography?
A) cGrowing ccrystal; cpurifying cprotein; cdiffracting ccrystal cusing cX-ray; csolving cthe cstructure
B) cPurifying cprotein; cgrowing ccrystal; cdiffracting ccrystal cusing cX-ray; csolving cthe cstructure
C) cPurifying cprotein; csolving cthe cstructure; cgrowing ccrystal; cdiffracting ccrystal cusing cX-ray
D) cGrowing ccrystal; cdiffracting ccrystal cusing cX-ray; cpurifying cprotein; csolving cthe cstructure c- cCO
20. cIn cgeneral, can canti-parallel cbeta csheet cis cslightly cmore cstable cthan ca cparallel cbeta csheet.
A) cAn canti-parallel cbeta csheet calways ccontains ca chigher ccontent cof chydrophobic cresidues
B) cAn canti-parallel cbeta csheet calways chas cmore cstrands
C) cAn canti-parallel cbeta csheet callows cthe cformation cof cstronger chydrogen cbonds
D) cAll cof cthe cabove c- cCORRECT cANS✔✔C
21. cA cmixture cof cfour cproteins c- cG cprotein c(92 ckDa), cGPCR c(50 ckDa), cMAPK c(35 ckDa), cand c
- cis capplied cto ca cgel-filtration ccolumn; cthe cprotein cthat ccomes cout cof cthe ccolumn cfirst c(the cear
A) cG cprotein c
B) cGPCR c
C) cMAPK c
D) cp53 c- cCORRECT cANS✔✔A
22. cA cmixture cof cfour cproteins c- cHistone cH1 c(pI c10.7), cp53 c(pI c6.83), cRas c(pI c6.33), cand ctryp
cto ca ccation-exchange ccolumn c(i.e., cthe cmatrix cis ccomposed cof cbeads cthat ccontain cnegative cc
cthe cproteins cwill ccome cout cof cthe ccolumn clast c(the clatest)?
A) cHistone cH1 c
B) cp53 c
C) cRas c
D) ctrypsinogen c- cCORRECT cANS✔✔A
23. cHemoglobin cis ca cprotein cwith ca ctotal cof cfour csubunits, ceach cwith ca cmolecular cweight cof c1
cof chemoglobin cis c6.8. cA crelated cprotein, cmyoglobin, cis ca cprotein cwith ca csingle cpolypeptide cch
cweight cof c16.7 ckDa cand can cisoelectric cpoint cof c6.87. cWhich cof cthe cfollowing cmethods ccan cbe
chemoglobin cand cmyoglobin?
A) cSDS-PAGE c
B) cisoelectric cfocusing c
C) c2-dimensional cgel c
D) cgel-filtration c- cCORRECT cANS✔✔D
24. cThe cinformation cthat ccan cbe cgained cfrom ca cWestern cblotting cexperiment cinclude
A) cthe cpresence cof ca cprotein cof cinterest cin cthe csample cand cthe cestimation cof cits cmolecular cw
B) cthe cabundance cof ca cprotein cof cinterest cin cthe csample cand cthe cestimation cof cits cisoelectric
C) cthe cpresence cof ca cspecific cDNA cfragment cin cthe csample
D) cthe cpresence cof ca cspecific cmRNA cin cthe csample c- cCORRECT cANS✔✔A
25. cIn cSDS-PAGE, cthe cfunction cof cSDS cis cto
A) cIncrease cthe csize cof cthe cprotein, cso cit ccan cmigrate cslower cin cthe celectric cfield
B) cReduce cthe cdisulfide cbonds cpresent cin cthe cprotein
C) cDenature cthe cprotein cand cgive cprotein cextensive cnegative ccharges
50 cmultiple cchoice cquestions, ccovering call cthe ctopics cwe've ctaught cyou cover cthe ccourse cof cthe
csplit cinto c4 csections: c1) cProteins c(10 cpoints) c2) cDNA c(10 cpoints), c3) cTranscription c& cTranslat
cEnergy c& cMetabolism c(20 cpoints). cThe cbest cway cto cprepare cfor cthis cexam cis cto creview cyour
c"big cpictures" cof ceach clecture cusing cthe c"specifics cto cknow" cprovided. cYou ccan cexpect c1 cor c
cCORRECT cANS✔✔
Exam c1 cversion cA c- cCORRECT cANS✔✔protein cstructure cand cfunction
Many ctypes cof cnon-covalent cinteractions care celectrostatic cin cnature; cone cnotable cexception cis
A) cHydrogen cbond c
C) cIonic cinteraction
B) cVan cder cWaals cinteraction c
D) cHydrophobic cinteraction c- cCORRECT cANS✔✔D
2. cMaintaining ccorrect cpH cis cvery cimportant cfor cliving csystems. cWhich cof cthe cfollowing cmolecu
important cfor cpH ccontrol cin cliving csystems?
A) cProteins, cespecially cthose cwith chigh ccontent cof chistidine cresidues
B) cDihydrogen cphosphate cand chydrogen cphosphate
C) cBicarbonate
D) cAll cof cthe cabove c- cCORRECT cANS✔✔D
3. cWhile cmany cbiochemists cuse cthe cterms c"aspartate" cand c"aspartic cacid" cinterchangeably, cthe
different cthings. cWhich cof cthe cfollowing cregarding cthese ctwo cterms cis ctrue?
A) cAspartate cis cthe cconjugate cbase cof caspartic cacid.
B) cAspartate cis cconverted cto caspartic cacid cupon can cincrease cin cpH.
C) cAspartic cacid cis cconverted cto caspartate cupon ca cdecrease cin cpH.
D) cAll cof cthe cabove. c- cCORRECT cANS✔✔A
4. cMost clikely, cthe cdiagram con cthe cright cillustrates
A) cA chydrogen cbond cformed cbetween ca cbase-pair
B) cA chydrogen cbond cformed cbetween ctwo cadjacent cbeta-strands
C) cAn cionic cinteraction cbetween ctwo cadjacent calpha chelices
D) cA chydrogen cbond cformed cbetween ctwo cadjacent calpha chelices c- cCORRECT cANS✔✔pictur
B
,D) cHistidine; cproton cdonor c- cCORRECT cANS✔✔picture
B
8. cCystine cis cformed cvia
A) cReduction cof cdisulfide cbond
B) cOxidation cof cthe cside cchain cof ctwo ccysteines
C) cDimerization cbetween ca ccysteine cand ca ctyrosine
D) cDimerization cbetween ca ccysteine cand ca cmethionine c- cCORRECT cANS✔✔B
9. cWhich cof cthe cfollowing cstatements cis ctrue cregarding cthe ctwo
amino cacids cshown con cthe cright?
A) cBoth ccan cbe csynthesized cusing cpyruvate cas ca cdirect cprecursor
B) cBoth ccan cbe csynthesized cusing coxaloacetate cas ca cdirect cprecursor
C) cThe cone con cthe cleft cbut cnot cthe cone con cthe cright ccan cbe
synthesized cusing cpyruvate cas ca cdirect cprecursor
D) cBoth care cD-aspartate c- cCORRECT cANS✔✔picture
B
10. cHistidine cis can camino cacid cwith cthree ctitratable cgroups: can c−NH3+ cgroup c(pKa=9.2), ca c−C
can cimidazole c(amine-like) cgroup c(pKa=6.0). cThe cpI cof chistidine cis
A) c5.5 c
B) c7.6 c
C) c3.9 c
D) c8.5 c- cCORRECT cANS✔✔B
11. cAs can cinhibitor cfor cthe cshikimate cpathway, cglyphosate c(roundup) cis cable cto ckill cweeds cbec
A) cIt cblocks cthe csynthesis cof cbranched cchain camino cacids csuch cas cIle cand cVal
B) cIt cblocks cthe cconversion cof calpha-ketoglutarate cto cglutamate
C) cIt cblocks cthe csynthesis cof caromatic camino cacids csuch cas cPhe cand cTrp
D) cIt cstimulates chuman cbeings cto cpull cthe cweeds cout cof ctheir cyard c- cCORRECT cANS✔✔C
12. cWhich cof cthe cfollowing csets cof camino cacids chave cthree cionizable cgroups?
A) cAla, cPro, cAsn
B) cAsn, cAsp, cGln
C) cAsp, cSer, cTrp
D) cAsp, cCys, cTyr c- cCORRECT cANS✔✔D
picture cof cpetptide cchain cwith cside cchains: cCH3, cCH2(ring)OH, cCH2COO-, cH
13. cThe csequence cof cthis cpeptide cis
A) cAFDG cB) cAYDG cC) cAYEG cD) cGYEA
14. cThe cisoelectric cpoint c(pI) cof cthe cpeptide cis:
A) c3.6 cB) c6.1 cC) c6.5 cD) c7.0 c- cCORRECT cANS✔✔sequence:
pI:
13) cB
14)a
15. cWhich cof cthe cfollowing cresidues care crarely cfound cin can calpha chelix?
A) cGlu, cArg, cLys
B) cTyr, cPhe, cLeu
, experiment, ctwo cchemical creagents c- curea cand cbeta-mercaptoethanol c- cwere cused. cThe cfuncti
is cto c_________ cand cthe cfunction cof cbeta-mercaptoethanol cis cto c______________. cBetween ct
reagents, cthis cone c_____________ cshould cbe cremoved cfirst cto censure ccorrect crefolding cof cthe
A) cdenature cthe cprotein; coxidize cthe cprotein; cbeta-mercaptoethanol
B) cdenature cthe cprotein; creduce cthe cdisulfide cbonds; curea
C) creduce cthe cdisulfide cbonds; cdenature cthe cprotein; cbeta-mercaptoethanol
D) csolubilize cthe cprotein; creduce cthe cdisulfide cbonds; curea c- cCORRECT cANS✔✔B
19. cWhich cof cthe cfollowing ccorrectly cdescribes cthe corder cof cevents cin cdetermining cprotein cstru
X-ray ccrystallography?
A) cGrowing ccrystal; cpurifying cprotein; cdiffracting ccrystal cusing cX-ray; csolving cthe cstructure
B) cPurifying cprotein; cgrowing ccrystal; cdiffracting ccrystal cusing cX-ray; csolving cthe cstructure
C) cPurifying cprotein; csolving cthe cstructure; cgrowing ccrystal; cdiffracting ccrystal cusing cX-ray
D) cGrowing ccrystal; cdiffracting ccrystal cusing cX-ray; cpurifying cprotein; csolving cthe cstructure c- cCO
20. cIn cgeneral, can canti-parallel cbeta csheet cis cslightly cmore cstable cthan ca cparallel cbeta csheet.
A) cAn canti-parallel cbeta csheet calways ccontains ca chigher ccontent cof chydrophobic cresidues
B) cAn canti-parallel cbeta csheet calways chas cmore cstrands
C) cAn canti-parallel cbeta csheet callows cthe cformation cof cstronger chydrogen cbonds
D) cAll cof cthe cabove c- cCORRECT cANS✔✔C
21. cA cmixture cof cfour cproteins c- cG cprotein c(92 ckDa), cGPCR c(50 ckDa), cMAPK c(35 ckDa), cand c
- cis capplied cto ca cgel-filtration ccolumn; cthe cprotein cthat ccomes cout cof cthe ccolumn cfirst c(the cear
A) cG cprotein c
B) cGPCR c
C) cMAPK c
D) cp53 c- cCORRECT cANS✔✔A
22. cA cmixture cof cfour cproteins c- cHistone cH1 c(pI c10.7), cp53 c(pI c6.83), cRas c(pI c6.33), cand ctryp
cto ca ccation-exchange ccolumn c(i.e., cthe cmatrix cis ccomposed cof cbeads cthat ccontain cnegative cc
cthe cproteins cwill ccome cout cof cthe ccolumn clast c(the clatest)?
A) cHistone cH1 c
B) cp53 c
C) cRas c
D) ctrypsinogen c- cCORRECT cANS✔✔A
23. cHemoglobin cis ca cprotein cwith ca ctotal cof cfour csubunits, ceach cwith ca cmolecular cweight cof c1
cof chemoglobin cis c6.8. cA crelated cprotein, cmyoglobin, cis ca cprotein cwith ca csingle cpolypeptide cch
cweight cof c16.7 ckDa cand can cisoelectric cpoint cof c6.87. cWhich cof cthe cfollowing cmethods ccan cbe
chemoglobin cand cmyoglobin?
A) cSDS-PAGE c
B) cisoelectric cfocusing c
C) c2-dimensional cgel c
D) cgel-filtration c- cCORRECT cANS✔✔D
24. cThe cinformation cthat ccan cbe cgained cfrom ca cWestern cblotting cexperiment cinclude
A) cthe cpresence cof ca cprotein cof cinterest cin cthe csample cand cthe cestimation cof cits cmolecular cw
B) cthe cabundance cof ca cprotein cof cinterest cin cthe csample cand cthe cestimation cof cits cisoelectric
C) cthe cpresence cof ca cspecific cDNA cfragment cin cthe csample
D) cthe cpresence cof ca cspecific cmRNA cin cthe csample c- cCORRECT cANS✔✔A
25. cIn cSDS-PAGE, cthe cfunction cof cSDS cis cto
A) cIncrease cthe csize cof cthe cprotein, cso cit ccan cmigrate cslower cin cthe celectric cfield
B) cReduce cthe cdisulfide cbonds cpresent cin cthe cprotein
C) cDenature cthe cprotein cand cgive cprotein cextensive cnegative ccharges
