ACS BIOCHEMISTRY FINAL.
ACTUAL EXAM STUDY GUIDE.
GRADED A+. WITH QUESTIONS
AND 100% VERIFIED
ANSWERS. LATEST 2025/2026
UPDATE
pKa (chapter 3) - Ans✔✔-measure of the tendency of a group to give up a
proton (acidity); tendency decreases tenfold as pKa increases by one unit
thioester bond (1) - Ans✔✔-compounds with functional group C-S-CoA-C (eg,
in acetyl-CoA)
zwitterion (3) - Ans✔✔-dipolar ion with both positive and negative groups but
overall neutral charge; can act as either an acid or base
Isoelectric point (pI) (chapter 3) - Ans✔✔-the characteristic pH at which the net
electric charge is zero
SDS - Sodium Dodecyl Sulfate (chap 3) - Ans✔✔-detergent used to unfold
proteins and give them uniform negative charge
, SDS Page (3) - Ans✔✔-chromatography used to separate proteins based on
mass. light proteins travel fast than heavier ones
PCR - Polymerase chain reaction (3) - Ans✔✔-copies DNA multiple times to
increase sample size
Isoelectric focusing (chapt 3) - Ans✔✔-procedure used to determine the
isoelectric pt (pI) of a protein. Protein migrates through gel until pH = pI (net
charge = 0)
Two-Dimensional Electrophoresis (3) - Ans✔✔-combines isoelectric focusing
and SDS electrophoresis; separates proteins by both molecular weight and pI
specific activity (3) - Ans✔✔-number of enzyme units per mg of total protein (a
measure of enzyme purity)
activity (3) - Ans✔✔-total units of a certain enzyme in a solution
Peptide bonds (chapter 4) - Ans✔✔-C-N bond with double bond character due
to resonance (C-N bond cannot rotate, and is planar)
Edman degredation (3) - Ans✔✔-used in the sequencing of polypeptides; labels
and removes ONLY the amino-residue from a polypeptide. carried out in a
machine called a sequenator
ACTUAL EXAM STUDY GUIDE.
GRADED A+. WITH QUESTIONS
AND 100% VERIFIED
ANSWERS. LATEST 2025/2026
UPDATE
pKa (chapter 3) - Ans✔✔-measure of the tendency of a group to give up a
proton (acidity); tendency decreases tenfold as pKa increases by one unit
thioester bond (1) - Ans✔✔-compounds with functional group C-S-CoA-C (eg,
in acetyl-CoA)
zwitterion (3) - Ans✔✔-dipolar ion with both positive and negative groups but
overall neutral charge; can act as either an acid or base
Isoelectric point (pI) (chapter 3) - Ans✔✔-the characteristic pH at which the net
electric charge is zero
SDS - Sodium Dodecyl Sulfate (chap 3) - Ans✔✔-detergent used to unfold
proteins and give them uniform negative charge
, SDS Page (3) - Ans✔✔-chromatography used to separate proteins based on
mass. light proteins travel fast than heavier ones
PCR - Polymerase chain reaction (3) - Ans✔✔-copies DNA multiple times to
increase sample size
Isoelectric focusing (chapt 3) - Ans✔✔-procedure used to determine the
isoelectric pt (pI) of a protein. Protein migrates through gel until pH = pI (net
charge = 0)
Two-Dimensional Electrophoresis (3) - Ans✔✔-combines isoelectric focusing
and SDS electrophoresis; separates proteins by both molecular weight and pI
specific activity (3) - Ans✔✔-number of enzyme units per mg of total protein (a
measure of enzyme purity)
activity (3) - Ans✔✔-total units of a certain enzyme in a solution
Peptide bonds (chapter 4) - Ans✔✔-C-N bond with double bond character due
to resonance (C-N bond cannot rotate, and is planar)
Edman degredation (3) - Ans✔✔-used in the sequencing of polypeptides; labels
and removes ONLY the amino-residue from a polypeptide. carried out in a
machine called a sequenator
