Bio 181 Lab Final Questions and Correct
Answers/ Latest Update / Already Graded
What is a catalyst?
Ans: A substance that increases the rate of a chemical reaction
without being used up
What is an enzyme?
Ans: A biological molecule that increases the rate of a chemical
reaction without being consumed, usually a protein.
What is the activation energy?
Ans: The energy required to convert a substrate to a product
How do enzymes catalyze reactions?
Ans: By forming a substrate-enzyme complex. The enzyme
brings the substrates together and put stress to break the
bonds. Enzymes create a micro-environment or break bonds
What are 7 properties of enzymes?
Ans: Most are proteins.
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Highly specific to - usually - one substrate.
They form an enzyme-substrate complex:
E + S ↔ ES → P + E
They do not affect the direction of a reaction.
They lower the energy of activation of a reaction.
They are not consumed in the reaction.
They are often highly regulated.
How does an inhibitor work?
Ans: Prevents binding of the target molecule of the enzyme by
binding to the active site or another site
What are the 2 types of inhibitors?
Ans: Competitive and Noncompetitive
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What affects enzymatic activity?
Ans: Substrate specificity, substrate/enzyme concentration, pH
and temperature
How does substrate specificity affect enzymatic activity?
Ans: Substrate specificity is a term for the fact that each
enzyme generally converts only one specific substrate for one
specific reaction. The more specific a substrate is, the enzyme
will want to react with that substrate
How does substrate/enzyme concentration affect enzymatic activity?
Ans: If there is too much substrate compared to the enzyme,
enzymatic activity will plateau
Increasing the amount of enzymes will increase the rate of
enzymatic activity
How does pH affect enzymatic activity?
Ans: Enzymes function in a certain pH, if the pH changes more
acidic or basic the enzyme's functional groups change. This
changes the structure. Extreme pH can denature enzymes by
breaking the hydrogen bonds between them
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