Cell Bio Exam 3 Dimario, chapter 8 Exam with Answers
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Terms in this set (30)
What kind of RNA does RNA - RNA polymerase I transcribed rRNAs
polymerase I transcribe? RNA - RNA polymerase II transcribes mRNAs
polymerase II and III? - RNA polymerase III transcribes tRNAs
Differential sensitivities to what Alpha aminitin
toxin allows you to determine - RNA Pol I has low sensitivity
which polymerase transcribes a - RNA Pol II has very high sensitivity
certain gene? - RNA Pol III intermediate
The Carboxyl terminal domain (CTD) in Pol II
Which domain is associated with
- The Tyr and Ser residues of CTD will be phosphorylated during
RNA Pol II and what is significant
transcription elongation (may be the trigger to begin
about it?
transcription)
What is the transcription control The TATA Box (within the promoter)
region (element) that positions - if sequences are deleted between TATA and true +1, initiation
the initial start site of will still be 18-26 bases down-stream of TATA
transcription at +1?
Just initiator elements
CpG-rich promoters for genes encoding enzymes for
What elements could Pol II
intermediary metabolism ("house-keeping genes")
genes contain other than TATA
- No TATA or initiator elements
elements? (2)
- (5-methyl C) --> CpG rich promoters where C's can be
methylated
What is significant about the CpG-rich promoters have bi-directional initiation (they can go in
direction that an RNA sequence each direction starting at the TSS-Transcription Start Site.)
can be transcribed in regard to
a CpG-rich promoter?
Chromatin Immuno-Precipitations (ChIPs): It localizes chromatin
protein of interest within the genome.
- First step is to treat living cells or tissues with formaledehyde (a
What technique is used to
membrane permeable cross-linker to cross-link proteins with
identify DNA sequences
DNA)
associated with defined
- Next step is to Sonicate the cells o shear cellular chromatin into
chromatin proteins and how
short fragments, then add antibody that binds to Pol II
does it work? (in the case we
- Third step is to immunoprecipitate to isolate the Poll II cross-
looked at, it was RNA Pol II)
linked to DNA
- The final step is to reverse the cross-linking (isolating the DNA)
and sequence this DNA using Next Gen Sequencing
What is the order in which the TFIID binds ---> then TFIIA and TFIIB bind ---> then TFIIF binds ---
Transcription Factor complexes > then TFIIE binds ---> and finally TFIIH binds
bind to the DNA sequence to
create the RNA Pol II Pre-
Transcription Initiation
Complex?
Save
Terms in this set (30)
What kind of RNA does RNA - RNA polymerase I transcribed rRNAs
polymerase I transcribe? RNA - RNA polymerase II transcribes mRNAs
polymerase II and III? - RNA polymerase III transcribes tRNAs
Differential sensitivities to what Alpha aminitin
toxin allows you to determine - RNA Pol I has low sensitivity
which polymerase transcribes a - RNA Pol II has very high sensitivity
certain gene? - RNA Pol III intermediate
The Carboxyl terminal domain (CTD) in Pol II
Which domain is associated with
- The Tyr and Ser residues of CTD will be phosphorylated during
RNA Pol II and what is significant
transcription elongation (may be the trigger to begin
about it?
transcription)
What is the transcription control The TATA Box (within the promoter)
region (element) that positions - if sequences are deleted between TATA and true +1, initiation
the initial start site of will still be 18-26 bases down-stream of TATA
transcription at +1?
Just initiator elements
CpG-rich promoters for genes encoding enzymes for
What elements could Pol II
intermediary metabolism ("house-keeping genes")
genes contain other than TATA
- No TATA or initiator elements
elements? (2)
- (5-methyl C) --> CpG rich promoters where C's can be
methylated
What is significant about the CpG-rich promoters have bi-directional initiation (they can go in
direction that an RNA sequence each direction starting at the TSS-Transcription Start Site.)
can be transcribed in regard to
a CpG-rich promoter?
Chromatin Immuno-Precipitations (ChIPs): It localizes chromatin
protein of interest within the genome.
- First step is to treat living cells or tissues with formaledehyde (a
What technique is used to
membrane permeable cross-linker to cross-link proteins with
identify DNA sequences
DNA)
associated with defined
- Next step is to Sonicate the cells o shear cellular chromatin into
chromatin proteins and how
short fragments, then add antibody that binds to Pol II
does it work? (in the case we
- Third step is to immunoprecipitate to isolate the Poll II cross-
looked at, it was RNA Pol II)
linked to DNA
- The final step is to reverse the cross-linking (isolating the DNA)
and sequence this DNA using Next Gen Sequencing
What is the order in which the TFIID binds ---> then TFIIA and TFIIB bind ---> then TFIIF binds ---
Transcription Factor complexes > then TFIIE binds ---> and finally TFIIH binds
bind to the DNA sequence to
create the RNA Pol II Pre-
Transcription Initiation
Complex?
