ACS Biochemistry First Semester
ACS Biochemistry First
Semester
, ACS Biochemistry First Semester
Buffer Order and Henderson Hasselbach: Water, Buffer, Salt, Base pH =
pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
1. Ramachandran plots: ¦ = N-R È = C-R
optimal angle to prevent steric hinderance
2. Protein structure of a helix, B sheet, reverse turn: a: LEMARKHQ
B: VICYFTW
RT: PSDNG
3. how many amino acids in alpha turn helix: 3.6
4. Super secondary structure: helix turn helix
5. parallel vs. antiparallel: parallel wedges face same way antiparallel face
opposite
, ACS Biochemistry First Semester
6. Isoelectric point (pI): pH at which a particular
molecule carries no net electrical charge.
7. Protein Separation Techniques (ALL): Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG
8. Protein Separation Techniques (H): Homogenization disrupt cells, sonicator,
centrifuge
9. Protein Separation Techniques (S): Salting out
ACS Biochemistry First
Semester
, ACS Biochemistry First Semester
Buffer Order and Henderson Hasselbach: Water, Buffer, Salt, Base pH =
pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
1. Ramachandran plots: ¦ = N-R È = C-R
optimal angle to prevent steric hinderance
2. Protein structure of a helix, B sheet, reverse turn: a: LEMARKHQ
B: VICYFTW
RT: PSDNG
3. how many amino acids in alpha turn helix: 3.6
4. Super secondary structure: helix turn helix
5. parallel vs. antiparallel: parallel wedges face same way antiparallel face
opposite
, ACS Biochemistry First Semester
6. Isoelectric point (pI): pH at which a particular
molecule carries no net electrical charge.
7. Protein Separation Techniques (ALL): Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG
8. Protein Separation Techniques (H): Homogenization disrupt cells, sonicator,
centrifuge
9. Protein Separation Techniques (S): Salting out
