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Study Guide
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - CORRECT - ANSWERS Nitrogen
Which of the proteins is soluble in water? - CORRECT - ANSWERS Albumin
How are amino acids joined together to form a chainlike structure? - CORRECT - ANSWERS
Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to? - CORRECT - ANSWERS
Alteration of tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - CORRECT - ANSWERS
Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - CORRECT - ANSWERS
Maple syrup disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - CORRECT - ANSWERS Five
Which reagent is employed in the serum protein determination? - CORRECT - ANSWERS
Biuret
,Why should hemolyzed serum not be used for total protein analysis? - CORRECT - ANSWERS
Hemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - CORRECT - ANSWERS It is
relatively free from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - CORRECT - ANSWERS Follows
Beer's law for a reasonable range of protein, depends on the presence of at least two peptide
linkages, and is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
CORRECT - ANSWERS There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - CORRECT - ANSWERS
Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - CORRECT - ANSWERS 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is
seen in which disease state? - CORRECT - ANSWERS Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma globulin, then what condition may exist in the patient? - CORRECT -
, ANSWERS Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - CORRECT -
ANSWERS Carbohydrates
All of the following are glycoproteins: - CORRECT - ANSWERS Ceruloplasmin, follicle-
stimulating hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - CORRECT - ANSWERS Cryoglobulin
Which one of the following applies to cryoglobulins? - CORRECT - ANSWERS They are
temperature-sensitive proteins
Which of the following statements is true of albumin? - CORRECT - ANSWERS It is
produced in the liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - CORRECT -
ANSWERS Down syndrome
Which one of the following proteins transports iron? - CORRECT - ANSWERS Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - CORRECT - ANSWERS
Blood urea nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - CORRECT -
ANSWERS Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxomine
method? - CORRECT - ANSWERS The method is not measuring ammonia