PCB 3063 Exam 3 – Genomics Questions
and Answers Graded A+
recombinant DNA is the ________ of DNA molecules. - Correct answer-joining
recombinant DNA is produced by - Correct answer-artificially joining DNA from
different biological sources not found together in nature
what is the procedure for generating recombinant DNA called - Correct answer-
cloning
clones are - Correct answer-recovered copies of a recombinant DNA molecule
recombinant DNA is used to ______, ____________, and _________ genes -
Correct answer-isolate, replicate, analyze
what are considered the cornerstones of recombinant DNA technology that led to
the formation of the modern biotechnology industry? - Correct answer-restriction
enzymes
what are restriction enzymes - Correct answer-DNA cutting enzymes
, where do restriction enzymes bind to DNA and what do they do there? - Correct
answer-bind to specific recognition sites (restriction site) and cleave DNA to
produce restriction fragments
do restriction enzymes cleave both or only one strand on DNA (digestion) - Correct
answer-both
what is the first step in producing recombinant DNA - Correct answer-1)
generating specific DNA fragments using restriction enzymes
what is the second step in producing recombinant DNA - Correct answer-2) joining
the fragment with a vector (plasmid, phage arm)
what is the last step in producing recombinant DNA - Correct answer-3)
transferring the recombinant DNA molecule to a host cell to produce many copies
that can be recovered from the host cell.
what are restriction enzymes produced by? - Correct answer-bacteria - as a defense
mechanism against bacteriophage
restriction-modification system protects the host DNA while doing what? - Correct
answer-destroying foreign DNA
- e. coli also produces methylases to modify those specific sites
and Answers Graded A+
recombinant DNA is the ________ of DNA molecules. - Correct answer-joining
recombinant DNA is produced by - Correct answer-artificially joining DNA from
different biological sources not found together in nature
what is the procedure for generating recombinant DNA called - Correct answer-
cloning
clones are - Correct answer-recovered copies of a recombinant DNA molecule
recombinant DNA is used to ______, ____________, and _________ genes -
Correct answer-isolate, replicate, analyze
what are considered the cornerstones of recombinant DNA technology that led to
the formation of the modern biotechnology industry? - Correct answer-restriction
enzymes
what are restriction enzymes - Correct answer-DNA cutting enzymes
, where do restriction enzymes bind to DNA and what do they do there? - Correct
answer-bind to specific recognition sites (restriction site) and cleave DNA to
produce restriction fragments
do restriction enzymes cleave both or only one strand on DNA (digestion) - Correct
answer-both
what is the first step in producing recombinant DNA - Correct answer-1)
generating specific DNA fragments using restriction enzymes
what is the second step in producing recombinant DNA - Correct answer-2) joining
the fragment with a vector (plasmid, phage arm)
what is the last step in producing recombinant DNA - Correct answer-3)
transferring the recombinant DNA molecule to a host cell to produce many copies
that can be recovered from the host cell.
what are restriction enzymes produced by? - Correct answer-bacteria - as a defense
mechanism against bacteriophage
restriction-modification system protects the host DNA while doing what? - Correct
answer-destroying foreign DNA
- e. coli also produces methylases to modify those specific sites
