ECC PRACTICE QUESTIONS AND ANSWERS
1. Regarding the confocal microscope demonstration of PH domain trafficking
to the membrane performed;
How might a conclusion on PH domain trafficking be compromised in the
absence of the box if the experiment were to be performed on an exposed or
open stage?- Answer - The box maintained a relatively air-tight compartment ensuring that temperature and
CO2 (37C and 5% respectively) fed into the chamber maintained adequate conditions for mammalian cell growth and
prevented light from entering into the chamber
2. Regarding the confocal microscope demonstration of PH domain trafficking
to the membrane performed;
What was the purpose of the big black box covering the microscope stage into
which the dish containing cells was placed?- Answer - the black box covering the microscope
compromised as they may relate only to cells grown under non-physiological conditions, the GFP signal could also be
compromised.
3. If it is possible to identify an excitation filter set that discriminates between
the mKO and mPlum excitation spectra, the emission of mKO after excitation
might be expected to?
a. Regenerate excitation of mKO in a positive feed-back fashion
b. Overlap substantially with the emission of mPlum
c. Induce emission of mPlum by exciting that fluorophore
d. Generate a fluorescence that we might perceive as being green- Answer
citation
- exfilter set
that discriminates between the mKO and mPlum excitation spectra, the emission of mKO after excitation is expected to
c. Induce emission of mPlum by exciting that fluorophore
4. If it is possible to identify an excitation filter set that discriminates between
mKO and mPlum state whether each of the following is True or False?
,1. A combination of a mKO exciter and mPlum barrier filter is a good combina-
tion for a FRET experiment involving proteins tagged with these two reporters
2. A combination of a mKO emitter and mPlum exciter filter is a good combina-
tion for a FRET experiment involving proteins tagged with these two reporters
3. A combination of a mKO emitter and mPlum exciter filter may be used in all
FRAP experiments to evaluate proteins tagged with mPlum- Answer - identification of an
excitation filter set that discriminates between mKO and mPlum infers,
1. True
, 2. False
3. False
5. 1. What is Nrf2?
A. One of the most unstable proteins in the cell
B. transcription factor which regulates the expression of cytoprotective genes
C. small molecule
D. component of edible plants- Answer - B. Nrf2 is a transcription factor which regulates the
expression of cytoprotective genes
6. What pathological conditions do we expect sulforaphane to protect against?
A. Cancer exclusively
B. Pathologies due to oxidative stress and chronic inflammation
C. Skin wounds
D. Autism exclusively- Answer - sulforaphane protects against B. Pathologies due to oxidative stress
and chronic inflammation
7. Considering the experimental evidence pertaining to the treatment of
autism, what is the most convincing evidence that the beneficial effects in the
autistic spectrum disorder clinical trial was due to intervention with the broccoli
sprout preparation?
A. The fact that the symptoms returned back to baseline after the intervention
was stopped
B. The fact that the symptoms in the placebo group stayed relatively constant
throughout the trial
C. The fact that the symptoms in the broccoli sprout preparation treatment
1. Regarding the confocal microscope demonstration of PH domain trafficking
to the membrane performed;
How might a conclusion on PH domain trafficking be compromised in the
absence of the box if the experiment were to be performed on an exposed or
open stage?- Answer - The box maintained a relatively air-tight compartment ensuring that temperature and
CO2 (37C and 5% respectively) fed into the chamber maintained adequate conditions for mammalian cell growth and
prevented light from entering into the chamber
2. Regarding the confocal microscope demonstration of PH domain trafficking
to the membrane performed;
What was the purpose of the big black box covering the microscope stage into
which the dish containing cells was placed?- Answer - the black box covering the microscope
compromised as they may relate only to cells grown under non-physiological conditions, the GFP signal could also be
compromised.
3. If it is possible to identify an excitation filter set that discriminates between
the mKO and mPlum excitation spectra, the emission of mKO after excitation
might be expected to?
a. Regenerate excitation of mKO in a positive feed-back fashion
b. Overlap substantially with the emission of mPlum
c. Induce emission of mPlum by exciting that fluorophore
d. Generate a fluorescence that we might perceive as being green- Answer
citation
- exfilter set
that discriminates between the mKO and mPlum excitation spectra, the emission of mKO after excitation is expected to
c. Induce emission of mPlum by exciting that fluorophore
4. If it is possible to identify an excitation filter set that discriminates between
mKO and mPlum state whether each of the following is True or False?
,1. A combination of a mKO exciter and mPlum barrier filter is a good combina-
tion for a FRET experiment involving proteins tagged with these two reporters
2. A combination of a mKO emitter and mPlum exciter filter is a good combina-
tion for a FRET experiment involving proteins tagged with these two reporters
3. A combination of a mKO emitter and mPlum exciter filter may be used in all
FRAP experiments to evaluate proteins tagged with mPlum- Answer - identification of an
excitation filter set that discriminates between mKO and mPlum infers,
1. True
, 2. False
3. False
5. 1. What is Nrf2?
A. One of the most unstable proteins in the cell
B. transcription factor which regulates the expression of cytoprotective genes
C. small molecule
D. component of edible plants- Answer - B. Nrf2 is a transcription factor which regulates the
expression of cytoprotective genes
6. What pathological conditions do we expect sulforaphane to protect against?
A. Cancer exclusively
B. Pathologies due to oxidative stress and chronic inflammation
C. Skin wounds
D. Autism exclusively- Answer - sulforaphane protects against B. Pathologies due to oxidative stress
and chronic inflammation
7. Considering the experimental evidence pertaining to the treatment of
autism, what is the most convincing evidence that the beneficial effects in the
autistic spectrum disorder clinical trial was due to intervention with the broccoli
sprout preparation?
A. The fact that the symptoms returned back to baseline after the intervention
was stopped
B. The fact that the symptoms in the placebo group stayed relatively constant
throughout the trial
C. The fact that the symptoms in the broccoli sprout preparation treatment
