AAB MT CHEMISTRY EXAM 2025/2026
QUESTIONS AND ANSWERS 100% PASS
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - ANS Nitrogen
Which of the proteins is soluble in water? - ANS Albumin
How are amino acids joined together to form a chainlike structure? - ANS Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to? - ANS Alteration of tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - ANS Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - ANS Maple syrup disease,
phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - ANS Five
Which reagent is employed in the serum protein determination? - ANS Biuret
Why should hemolyzed serum not be used for total protein analysis? - ANS Hemoglobin is
absorbed at the same wavelength as protein in the reagent
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Which of the following is NOT true of the biuret reaction? - ANS It is relatively free from
interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - ANS Follows Beer's law for a
reasonable range of protein, depends on the presence of at least two peptide linkages, and is
insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
ANS There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANS Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - ANS 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is
seen in which disease state? - ANS Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma globulin, then what condition may exist in the patient? -
ANS Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - ANS Carbohydrates
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, All of the following are glycoproteins: - ANS Ceruloplasmin, follicle-stimulating hormone
(FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - ANS Cryoglobulin
Which one of the following applies to cryoglobulins? - ANS They are temperature-sensitive
proteins
Which of the following statements is true of albumin? - ANS It is produced in the liver and is
water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - ANS Down
syndrome
Which one of the following proteins transports iron? - ANS Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - ANS Blood urea nitrogen
(BUN)
By the urease method, urea is enzymatically converted to which end product? -
ANS Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxomine
method? - ANS The method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values? -
ANS Elevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - ANS Jaffe
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
QUESTIONS AND ANSWERS 100% PASS
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - ANS Nitrogen
Which of the proteins is soluble in water? - ANS Albumin
How are amino acids joined together to form a chainlike structure? - ANS Peptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to? - ANS Alteration of tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - ANS Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - ANS Maple syrup disease,
phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - ANS Five
Which reagent is employed in the serum protein determination? - ANS Biuret
Why should hemolyzed serum not be used for total protein analysis? - ANS Hemoglobin is
absorbed at the same wavelength as protein in the reagent
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Which of the following is NOT true of the biuret reaction? - ANS It is relatively free from
interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - ANS Follows Beer's law for a
reasonable range of protein, depends on the presence of at least two peptide linkages, and is
insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
ANS There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANS Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - ANS 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is
seen in which disease state? - ANS Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10% beta-
globulin, and 45% gamma globulin, then what condition may exist in the patient? -
ANS Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - ANS Carbohydrates
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, All of the following are glycoproteins: - ANS Ceruloplasmin, follicle-stimulating hormone
(FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - ANS Cryoglobulin
Which one of the following applies to cryoglobulins? - ANS They are temperature-sensitive
proteins
Which of the following statements is true of albumin? - ANS It is produced in the liver and is
water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - ANS Down
syndrome
Which one of the following proteins transports iron? - ANS Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - ANS Blood urea nitrogen
(BUN)
By the urease method, urea is enzymatically converted to which end product? -
ANS Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxomine
method? - ANS The method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values? -
ANS Elevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - ANS Jaffe
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
