ASCP Chemistry Exam (2025/2026 Latest Exam
Prep) | Complete Certification Study Guide &
Verified Practice Q&A
In absorption spectrophotometry, what relationship is absorption to concentration? -
correct answerAbsorption is directly proportional to concentration.
What is the equation for Beer's law? - correct answerA= a x b x c
Where "a" equals absorptivity coefficient (a constant); "b" is the path length and "c" is
concentration.
A red solution transmits light at.
And strongly absorbs light at. - correct answerTransmits light at 600-650 nm.
Strongly absorbs light at 400-500 nm.
A green colored solution would show the highest transmittance between: - correct
answer500-550 nm
Which two hormones promote glyconeogensis and promote hyperglycemia? - correct
answerGrowth hormone.
And.
Cortisol.
Which hormone stimulates glycogenolysis? - correct answerEpinephrine.
What HLA antigens are genetically associated with type 1 diabetes. - correct
answerDR3.
And.
DR4.
What is the primary mineralocorticoid hormone that stimulates sodium reabsorption and
potassium secretion by the kidneys. - correct answerAldosterone.
Does excess thyroid hormone cause hyperglycemia or hypoglycemia? - correct
answerHyperglycemia.
What hormone opposes the action of parathyroid hormone? - correct answerCalcitonin.
,What hormone is released from the kidney due to ineffective arterial pressure and
promotes activation of angiotensinogen and aldosterone secretion? - correct
answerRenin.
In type 1 diabetes, what is required to prevent ketosis? - correct answerInsulin
replacement.
Excess of this hormone causes hyperglycemia by increasing glucagon and inactivation
of insulin; thereby promoting both gluconeogenesis and glycogenolysis. - correct
answerThyroid hormone.
What is type 2 diabetes associated with? - correct answerA defect in the receptor site
for insulin.
Usually occurs in patient over 40 and who are obese.
What is required of the patient's diet when getting the O.G.T.T -O'Sullivan Glucose
Tolerance Test? - correct answerRequired the patient receive at least 150g of
carbohydrates per day for 3 days prior to test.
When should the G.T.T -Glucose Tolerance Test be done? - correct answerIt should be
reserved for confirmation of diabetes and for difficult to diagnosis patients.
It is not longer recommended for screening purposes.
What criteria is used for diagnosis of diabetes mellitus for a fasting glucose? - correct
answergreater than or equal to 126 mg/dL on more than a single testing event.
What criteria is used for diagnosis of diabetes mellitus for a random glucose? - correct
answerGreater than or equal to 200 mg/dL on more than a single testing event.
What criteria is used for diagnosis of diabetes mellitus after 2 hours following ingestion
of 75 grams of glucose load? - correct answerA glucose greater than or equal to 200
mg/dL on more than a single testing event.
An A1C greater than or equal to what on more than a single testing event is diagnostic
of diabetes mellitus? - correct answer6.5.
What is the definition of Impaired Glucose Tolerance ? - correct answerIt is serum or
plasma glucose at 2 hours after a 75 g glucose load of greater than or equal to 140
mg/dL and less than 200 mg/dL.
What is GDM? - correct answerGestational diabetes mellitus and it is defined as
glucose intolerance during pregnancy.
When are pregnant women screened for gestational diabetes mellitus? - correct
answerBetween 24 and 28 weeks of gestation.
,What does the screening test for gestational diabetes mellitus consist of? - correct
answerAn oral 50 g glucose load followed by a glucose measurement at 1 hour.
A result greater than or equal to 140 mg/dL is followed by a 2 hr. or 3 hr. G.T.T to
confirm gestational diabetes.
If a 3 hour G.T.T is ordered, what amount of glucose load should be given? - correct
answer100 g dose.
What are the cutoff points for the glucose tolerance test for the diagnosis of gestational
diabetes - correct answerFasting - greater to or equal to 95 mg/dL.
1 hour- greater than or equal to 180 mg/dL.
2 hour- greater than or equal to 155 mg/dL.
3 hour - greater than or equal to 140 mg/dL.
At least two cutoffs must be exceeded.
What can cause clinical hypoglycemia? - correct answerInsulinoma.
Drugs.
Alcoholism.
Reactive hypoglycemia.
What is reactive hypoglycemia characterized by? - correct answerBy delayed or
excessive insulin output after eating.
Fasting insulin is normal, but post prandial levels are increased.
What is glycated hemoglobin? (G-Hgb) - correct answerIt is related to the time averaged
blood glucose concentration over the life span of the RBC.
How many G-Hgb fractions are there? - correct answer3 fractions:
A1a.
A1b.
A1c
What percentage does A1c make up of the glycated hemoglobin? - correct answer80%.
What is the formula for the time averaged blood glucose? - correct answer(A1c x 33.3) -
86 mg/dL.
What is the A.D.A recommended cutoff value for adequate control of blood glucose in
diabetes as measured by glycated hemoglobin? - correct answer6.5%.
Should the patient be fasting to have an A1c drawn? - correct answerNo, since A1c
represents the average blood glucose 2-3 months prior to collection, fasting on the day
of the test has no effect on the results.
What is considered the normal range for A1c? - correct answer4.0-5.5%
, What is the recommended cutoff for the early detection of chronic kidney disease in
diabetics using the test for microalbuminuria? - correct answer> 30 mg/g creatinine.
What is microalbuminuria? - correct answerExcretion of small quantities of albumin in
the urine.
For diabetics, when should they be tested for microalbuminuria? - correct
answerAnnually for all type 1 and type 2 diabetics who have had the disease > 5 years.
Why does the ADA not recommend the use of whole blood glucose monitors for
establishing a diagnosis of diabetes or for screening persons with diabetes? - correct
answerBecause whole blood glucose is approximately 10% lower than serum or plasma
glucose.
What is the reference method for measuring serum glucose? - correct
answerHexokinase.
Polarographic methods for glucose analysis are based upon: - correct answerThe rate
of O2 depletion.
What is amperometry? - correct answerdetection of ions in a solution based on electric
current or changes in electric current.
It's another method used to measure glucose in plasma.
What enzyme is most specific for beta-D-glucose? - correct answerGlucose oxidase.
What is the coupling enzyme used in the hexokinase method for glucose? - correct
answerGlucose-6-phosphate dehydrogenase
CSF glucose levels below 40 mg/dL occur in: - correct answerSeptic meningitis.
Cancer.
Multiple sclerosis.
What is the different between CSF glucose and plasma glucose? - correct answerCSF
glucose is usually 50-65% of the plasma glucose.
In the peroxidase coupled glucose methods, what reagent complexes with the
chromogen? - correct answerPhenol.
What effect does hematocrit have on POCT tests for whole blood glucose monitoring. -
correct answerA high hematocrit lowers the glucose because RBC glucose
concentration is lower than plasma concentration.
Point of care tests for whole blood glucose monitoring is basing mainly on the use of: -
correct answerAmperometric detection.
Prep) | Complete Certification Study Guide &
Verified Practice Q&A
In absorption spectrophotometry, what relationship is absorption to concentration? -
correct answerAbsorption is directly proportional to concentration.
What is the equation for Beer's law? - correct answerA= a x b x c
Where "a" equals absorptivity coefficient (a constant); "b" is the path length and "c" is
concentration.
A red solution transmits light at.
And strongly absorbs light at. - correct answerTransmits light at 600-650 nm.
Strongly absorbs light at 400-500 nm.
A green colored solution would show the highest transmittance between: - correct
answer500-550 nm
Which two hormones promote glyconeogensis and promote hyperglycemia? - correct
answerGrowth hormone.
And.
Cortisol.
Which hormone stimulates glycogenolysis? - correct answerEpinephrine.
What HLA antigens are genetically associated with type 1 diabetes. - correct
answerDR3.
And.
DR4.
What is the primary mineralocorticoid hormone that stimulates sodium reabsorption and
potassium secretion by the kidneys. - correct answerAldosterone.
Does excess thyroid hormone cause hyperglycemia or hypoglycemia? - correct
answerHyperglycemia.
What hormone opposes the action of parathyroid hormone? - correct answerCalcitonin.
,What hormone is released from the kidney due to ineffective arterial pressure and
promotes activation of angiotensinogen and aldosterone secretion? - correct
answerRenin.
In type 1 diabetes, what is required to prevent ketosis? - correct answerInsulin
replacement.
Excess of this hormone causes hyperglycemia by increasing glucagon and inactivation
of insulin; thereby promoting both gluconeogenesis and glycogenolysis. - correct
answerThyroid hormone.
What is type 2 diabetes associated with? - correct answerA defect in the receptor site
for insulin.
Usually occurs in patient over 40 and who are obese.
What is required of the patient's diet when getting the O.G.T.T -O'Sullivan Glucose
Tolerance Test? - correct answerRequired the patient receive at least 150g of
carbohydrates per day for 3 days prior to test.
When should the G.T.T -Glucose Tolerance Test be done? - correct answerIt should be
reserved for confirmation of diabetes and for difficult to diagnosis patients.
It is not longer recommended for screening purposes.
What criteria is used for diagnosis of diabetes mellitus for a fasting glucose? - correct
answergreater than or equal to 126 mg/dL on more than a single testing event.
What criteria is used for diagnosis of diabetes mellitus for a random glucose? - correct
answerGreater than or equal to 200 mg/dL on more than a single testing event.
What criteria is used for diagnosis of diabetes mellitus after 2 hours following ingestion
of 75 grams of glucose load? - correct answerA glucose greater than or equal to 200
mg/dL on more than a single testing event.
An A1C greater than or equal to what on more than a single testing event is diagnostic
of diabetes mellitus? - correct answer6.5.
What is the definition of Impaired Glucose Tolerance ? - correct answerIt is serum or
plasma glucose at 2 hours after a 75 g glucose load of greater than or equal to 140
mg/dL and less than 200 mg/dL.
What is GDM? - correct answerGestational diabetes mellitus and it is defined as
glucose intolerance during pregnancy.
When are pregnant women screened for gestational diabetes mellitus? - correct
answerBetween 24 and 28 weeks of gestation.
,What does the screening test for gestational diabetes mellitus consist of? - correct
answerAn oral 50 g glucose load followed by a glucose measurement at 1 hour.
A result greater than or equal to 140 mg/dL is followed by a 2 hr. or 3 hr. G.T.T to
confirm gestational diabetes.
If a 3 hour G.T.T is ordered, what amount of glucose load should be given? - correct
answer100 g dose.
What are the cutoff points for the glucose tolerance test for the diagnosis of gestational
diabetes - correct answerFasting - greater to or equal to 95 mg/dL.
1 hour- greater than or equal to 180 mg/dL.
2 hour- greater than or equal to 155 mg/dL.
3 hour - greater than or equal to 140 mg/dL.
At least two cutoffs must be exceeded.
What can cause clinical hypoglycemia? - correct answerInsulinoma.
Drugs.
Alcoholism.
Reactive hypoglycemia.
What is reactive hypoglycemia characterized by? - correct answerBy delayed or
excessive insulin output after eating.
Fasting insulin is normal, but post prandial levels are increased.
What is glycated hemoglobin? (G-Hgb) - correct answerIt is related to the time averaged
blood glucose concentration over the life span of the RBC.
How many G-Hgb fractions are there? - correct answer3 fractions:
A1a.
A1b.
A1c
What percentage does A1c make up of the glycated hemoglobin? - correct answer80%.
What is the formula for the time averaged blood glucose? - correct answer(A1c x 33.3) -
86 mg/dL.
What is the A.D.A recommended cutoff value for adequate control of blood glucose in
diabetes as measured by glycated hemoglobin? - correct answer6.5%.
Should the patient be fasting to have an A1c drawn? - correct answerNo, since A1c
represents the average blood glucose 2-3 months prior to collection, fasting on the day
of the test has no effect on the results.
What is considered the normal range for A1c? - correct answer4.0-5.5%
, What is the recommended cutoff for the early detection of chronic kidney disease in
diabetics using the test for microalbuminuria? - correct answer> 30 mg/g creatinine.
What is microalbuminuria? - correct answerExcretion of small quantities of albumin in
the urine.
For diabetics, when should they be tested for microalbuminuria? - correct
answerAnnually for all type 1 and type 2 diabetics who have had the disease > 5 years.
Why does the ADA not recommend the use of whole blood glucose monitors for
establishing a diagnosis of diabetes or for screening persons with diabetes? - correct
answerBecause whole blood glucose is approximately 10% lower than serum or plasma
glucose.
What is the reference method for measuring serum glucose? - correct
answerHexokinase.
Polarographic methods for glucose analysis are based upon: - correct answerThe rate
of O2 depletion.
What is amperometry? - correct answerdetection of ions in a solution based on electric
current or changes in electric current.
It's another method used to measure glucose in plasma.
What enzyme is most specific for beta-D-glucose? - correct answerGlucose oxidase.
What is the coupling enzyme used in the hexokinase method for glucose? - correct
answerGlucose-6-phosphate dehydrogenase
CSF glucose levels below 40 mg/dL occur in: - correct answerSeptic meningitis.
Cancer.
Multiple sclerosis.
What is the different between CSF glucose and plasma glucose? - correct answerCSF
glucose is usually 50-65% of the plasma glucose.
In the peroxidase coupled glucose methods, what reagent complexes with the
chromogen? - correct answerPhenol.
What effect does hematocrit have on POCT tests for whole blood glucose monitoring. -
correct answerA high hematocrit lowers the glucose because RBC glucose
concentration is lower than plasma concentration.
Point of care tests for whole blood glucose monitoring is basing mainly on the use of: -
correct answerAmperometric detection.
