ASCP BLOOD BANK EXAM QUESTIONS WITH ANSWERS

ASCP BLOOD BANK EXAM QUESTIONS |\ |\ |\ |\ |\




WITH ANSWERS |\




serum contains this ab more than others - CORRECT ANSWERS
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✔✔IgG


this ab is majorly found in body secretions - CORRECT ANSWERS
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✔✔IgA


the ___ portion of an antibody is responsible for placental transfer
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of IgG, is responsible for complement fixation and causes
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problems for the baby - CORRECT ANSWERS ✔✔Fc portion of IgG
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these antibodies activate the classical complement pathway -
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CORRECT ANSWERS ✔✔IgG, IgM |\ |\ |\




___ ab reacts at 37C (body temp) while _____ ab reacts at room
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temp - CORRECT ANSWERS ✔✔IgG (body), IgM (room)
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IgM - CORRECT ANSWERS ✔✔-can activate classical pathway of
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complement system |\




-only one molecule is necessary to activate complement
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-results in hemolysis of RBCs and intravascular destruction (IV
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hemolysis)
-ABO abs are typically IgM and can cause rapid hemolysis if an
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incompatible unit is transfused |\ |\ |\

,-expected in plasma, important in reverse typing
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-these abs are IgM: Lewis, Ii, P, MNS
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-react best at ambient/room temp (22-24C)
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-can interfere with detection of clinically significant IgG abs by
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masking reactivity |\ |\




-exist in moth monomeric and polymeric forms as pentamers
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containing a J chain. These large molecules cause visible
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agglutination reactions of ag-positive RBCs in saline |\ |\ |\ |\ |\ |\ |\




-pentameric form can be dissociated through cleavage of its |\ |\ |\ |\ |\ |\ |\ |\ |\


covalent bonds at the J chain using sulfhydral reducing agents
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-IgM is removed by treatment with 2-ME and DTT
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IgA - CORRECT ANSWERS ✔✔-exists in both monomer and
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polymer form as dimers or trimers composed of identical
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monomers
-about 30% of anti-A and anti-B antibodies are IgA class
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-present in large amounts in body secretions
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IgE - CORRECT ANSWERS ✔✔-found only in monomeric form in
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trace concentrations in serum. Important in allergic reactions
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-Fc portion of IgE molecule attaches to basophils and mast cells,
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which facilitates histamine release
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-not known for hemolytic transfusion reactions
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intravascular hemolysis occurs with ___ antibodies - CORRECT |\ |\ |\ |\ |\ |\ |\ |\


ANSWERS ✔✔-alloantibodies if complement is triggered
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,alloantibodies - CORRECT ANSWERS ✔✔-antibodies produced |\ |\ |\ |\ |\ |\


after exposure to genetically different, non-self of the same
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species


Paroxysmal cold hemoglobinuria occurs with ____ - CORRECT
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ANSWERS ✔✔autoantibodies- autoimmune disorder under cold
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temperatures.


extravascular hemolysis - CORRECT ANSWERS ✔✔-occurs in the|\ |\ |\ |\ |\ |\ |\ |\


liver/spleen |\




-occurs as a result of abs coating RBCs (either self or non-self)
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-removed by mononuclar phagocyte system
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-split products of complement activation can stimulate the
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reticuloendothelial system (RES) |\ |\




human leukocyte antigens (HLAs) are _____ - CORRECT ANSWERS
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✔✔glycoproteins
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most clinically significant blood group antigens and
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immunogenicity (chance of body making antibody against it) - |\ |\ |\ |\ |\ |\ |\ |\ |\


CORRECT ANSWERS ✔✔-D (Rh) -50% |\ |\ |\ |\




-K (Kell) - 5%
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-c (Rh)-2.05
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-E (Rh) -decreased from here
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-k (kell)
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-e (Rh)
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-Fya (Duffy)
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, -C (Rh)
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-Jka (Kidd)
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-S (MNSs)
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-Jkb (Kidd)
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-s (MNSs)
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antibodies against this blood group antigen tends to fall below
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detection limit of titer - CORRECT ANSWERS ✔✔Kidd (Jka)
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** ask if patient has ever received a transfusion and from where!!
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factors that influence Ag-Ab reactions - CORRECT ANSWERS ✔✔-
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centrifugation
-Ag-Ab ratio |\




-pH |\




-temperature


IgM antibodies that typically react in the immediate spin phase -
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CORRECT ANSWERS ✔✔A, B, H, I, P1, M, N, Le^a, Le^b
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IgG antibodies that react after the antiglobulin phase (37C) -
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CORRECT ANSWERS ✔✔D, C, E, c, e, K, Fy, Jk, S, s, Le^1, Le^b
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Factors that influence agglutination reactions - CORRECT
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ANSWERS ✔✔-22% albumin |\ |\




-LISS
-PeG