Molecular Biology Exam test quiz
and answers graded A+
In regards to epigenome sequencing techniques, if a cytosine is NOT methylated it will be converted
to __________ if subjected to bisulfite treatment. - ANS✅✅Uracil
If the DNA of a gene-rich region of a genome is heavily methylated most likely what will be the
result? - ANS✅✅Gene expression will be suppressed
In studies with mice the methylation status of the Agouti gene determines coat color (T or F) -
ANS✅✅True
Epigenetic histone tail modifications can result in either suppression or activation of genes (T or F) -
ANS✅✅True
In studies with Agouti mice, pregnant mice exposed to BPA and fed diets rich in genistein (a
metabolite from soy), were more likely to have obese light-colored offspring (T or F) -
ANS✅✅False
ORF scanning in prokaryotes typically relies on __________. - ANS✅✅Computer programs looking
for both translational start and stop codons
Illumina technology for next generation sequencing relies on __________. - ANS✅✅Bridge
amplification
Reversible terminators
In cycle sequencing one uses a polymerase like Taq (T or F) - ANS✅✅True
When using computers to predict gene models in genomes of eukaryotes it may be difficult because
__________. - ANS✅✅Intron exon junctions are not always obvious and easy to predict
Multiple ATGs may exist in the 5' sequence making it difficult to know the exact start codon
and answers graded A+
In regards to epigenome sequencing techniques, if a cytosine is NOT methylated it will be converted
to __________ if subjected to bisulfite treatment. - ANS✅✅Uracil
If the DNA of a gene-rich region of a genome is heavily methylated most likely what will be the
result? - ANS✅✅Gene expression will be suppressed
In studies with mice the methylation status of the Agouti gene determines coat color (T or F) -
ANS✅✅True
Epigenetic histone tail modifications can result in either suppression or activation of genes (T or F) -
ANS✅✅True
In studies with Agouti mice, pregnant mice exposed to BPA and fed diets rich in genistein (a
metabolite from soy), were more likely to have obese light-colored offspring (T or F) -
ANS✅✅False
ORF scanning in prokaryotes typically relies on __________. - ANS✅✅Computer programs looking
for both translational start and stop codons
Illumina technology for next generation sequencing relies on __________. - ANS✅✅Bridge
amplification
Reversible terminators
In cycle sequencing one uses a polymerase like Taq (T or F) - ANS✅✅True
When using computers to predict gene models in genomes of eukaryotes it may be difficult because
__________. - ANS✅✅Intron exon junctions are not always obvious and easy to predict
Multiple ATGs may exist in the 5' sequence making it difficult to know the exact start codon
