Molecular Biology Exam test queries
and answers graded A+
The three steps of transcription common to both prokaryotic and eukaryotic organisms include all of
the following except: - ANS✅✅Splicing
The steps of transcription include all the following except: - ANS✅✅Translocation
In prokaryotes, transcription starts when RNA polymerase binds to the - ANS✅✅Promoter
Which statement best describes the role of the eubacterial RNA polymerase sigma factor? -
ANS✅✅Sigma factor increases the affinity of RNA polymerase for promoter sequences
In general, termination of transcription in bacteria - ANS✅✅Requires transcription of the
sequences signaling termination
One major difference between bacterial and eukaryotic RNA polymerase is that -
ANS✅✅Eukaryotes have several RNA polymerases
Eukaryotic RNA polymerase II synthesizes - ANS✅✅mRNA
TATA-binding protein (TBP) is required for initiation by - ANS✅✅RNA polymerases I, II and III
The TATA box is a common component of promoters for - ANS✅✅RNA polymerase II
Which of the following general transcription factors phosphorylates the Pol II CTD - ANS✅✅TFIIH
RNA polymerase III may recognize promoter sequences that are entirely downstream from the start
point of transcription. - ANS✅✅True
MRNA is generally the most abundant type of RNA in a eukaryotic cell - ANS✅✅False (its rRNA)
, RNA processing includes all the following except - ANS✅✅Histone methylation
(It does include 5' capping, 3' polyadenylation and splicing)
The stem-loop structure at the 3' end of a bacterial mRNA functions primarily in -
ANS✅✅Termination of transcription
Single genes can produce multiple products via a type of RNA splicing known as -
ANS✅✅Alternative splicing
Prokaryotic mRNAs contain a ribosomal binding site that recruits the ribosome small subunit via
base pair interactions with the - ANS✅✅16s rRNA
The process of intron removal is RNA - ANS✅✅Splicing
Pre-mRNA splicing does not involve which of the following structural features? - ANS✅✅5' cap
(Includes 5' splice site, 3' splice site, and branch site)
U1 snRNA has a single-stranded 5' end that is able to base-pair with the - ANS✅✅5' splice site
Which of the following statements about splicing is false? - ANS✅✅Splicing can influence local
chromatin remodeling
Which of the following statements about splicing is true? - ANS✅✅-splicing is connected to mRNA
stability
-splicing can influence translation of the mRNA
-splicing can occur during transcription
Splicing is connected to mRNA export from the nucleus
The excision of a major intron initially forms a __________ structure where the 5' end of the intron
remains covalently bonded to the branch site. - ANS✅✅Lariat
SR proteins recruit splicing machinery to nearby splice sites. - ANS✅✅True
and answers graded A+
The three steps of transcription common to both prokaryotic and eukaryotic organisms include all of
the following except: - ANS✅✅Splicing
The steps of transcription include all the following except: - ANS✅✅Translocation
In prokaryotes, transcription starts when RNA polymerase binds to the - ANS✅✅Promoter
Which statement best describes the role of the eubacterial RNA polymerase sigma factor? -
ANS✅✅Sigma factor increases the affinity of RNA polymerase for promoter sequences
In general, termination of transcription in bacteria - ANS✅✅Requires transcription of the
sequences signaling termination
One major difference between bacterial and eukaryotic RNA polymerase is that -
ANS✅✅Eukaryotes have several RNA polymerases
Eukaryotic RNA polymerase II synthesizes - ANS✅✅mRNA
TATA-binding protein (TBP) is required for initiation by - ANS✅✅RNA polymerases I, II and III
The TATA box is a common component of promoters for - ANS✅✅RNA polymerase II
Which of the following general transcription factors phosphorylates the Pol II CTD - ANS✅✅TFIIH
RNA polymerase III may recognize promoter sequences that are entirely downstream from the start
point of transcription. - ANS✅✅True
MRNA is generally the most abundant type of RNA in a eukaryotic cell - ANS✅✅False (its rRNA)
, RNA processing includes all the following except - ANS✅✅Histone methylation
(It does include 5' capping, 3' polyadenylation and splicing)
The stem-loop structure at the 3' end of a bacterial mRNA functions primarily in -
ANS✅✅Termination of transcription
Single genes can produce multiple products via a type of RNA splicing known as -
ANS✅✅Alternative splicing
Prokaryotic mRNAs contain a ribosomal binding site that recruits the ribosome small subunit via
base pair interactions with the - ANS✅✅16s rRNA
The process of intron removal is RNA - ANS✅✅Splicing
Pre-mRNA splicing does not involve which of the following structural features? - ANS✅✅5' cap
(Includes 5' splice site, 3' splice site, and branch site)
U1 snRNA has a single-stranded 5' end that is able to base-pair with the - ANS✅✅5' splice site
Which of the following statements about splicing is false? - ANS✅✅Splicing can influence local
chromatin remodeling
Which of the following statements about splicing is true? - ANS✅✅-splicing is connected to mRNA
stability
-splicing can influence translation of the mRNA
-splicing can occur during transcription
Splicing is connected to mRNA export from the nucleus
The excision of a major intron initially forms a __________ structure where the 5' end of the intron
remains covalently bonded to the branch site. - ANS✅✅Lariat
SR proteins recruit splicing machinery to nearby splice sites. - ANS✅✅True
