Molecular & Cellular Biology Exam
questions and answers graded A+
what does the "P" in "PCR" stand for? - ANS✅✅polymerase
what does the "C" in "PCR" stand for? - ANS✅✅chain
what does the "R" in "PCR" stand for? - ANS✅✅reaction
what enzyme catalyzes PCR? - ANS✅✅DNA polymerase
how many steps are in 1 cycle of PCR? - ANS✅✅3
if you start with 1 copy of DNA, how many copies should there be after 4 cycles of PCR? -
ANS✅✅16
how many primers are typically needed for PCR? - ANS✅✅2
for the denaturation stepf of PCR, approximately what temperature is used? - ANS✅✅96 C
denaturing DNA means... - ANS✅✅breaking the hydrogen bonds between the bases
what metal ion is essential for PCR? - ANS✅✅Mg2+
when primers bind to their complementary sequences it is called... - ANS✅✅annealing
primers are extended... - ANS✅✅only 5' to 3' direction
what enzyme is used in PCR during the first cycle to add nucleotides to the 2nd strand? -
ANS✅✅tac polymerase
questions and answers graded A+
what does the "P" in "PCR" stand for? - ANS✅✅polymerase
what does the "C" in "PCR" stand for? - ANS✅✅chain
what does the "R" in "PCR" stand for? - ANS✅✅reaction
what enzyme catalyzes PCR? - ANS✅✅DNA polymerase
how many steps are in 1 cycle of PCR? - ANS✅✅3
if you start with 1 copy of DNA, how many copies should there be after 4 cycles of PCR? -
ANS✅✅16
how many primers are typically needed for PCR? - ANS✅✅2
for the denaturation stepf of PCR, approximately what temperature is used? - ANS✅✅96 C
denaturing DNA means... - ANS✅✅breaking the hydrogen bonds between the bases
what metal ion is essential for PCR? - ANS✅✅Mg2+
when primers bind to their complementary sequences it is called... - ANS✅✅annealing
primers are extended... - ANS✅✅only 5' to 3' direction
what enzyme is used in PCR during the first cycle to add nucleotides to the 2nd strand? -
ANS✅✅tac polymerase
