Ascp Mls Reviewed Questions And
Answers Graded A+ 2025-2026
What feature is most often found with patients with a splenectomy on a Wright's
stained blood smear? - ANS-Howell-Jolly bodies
Patients with antibody to the following antigen are immune to Hepatitis B: - ANS-
Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous Streptococcal
throat and skin infections: - ANS-ASO titer and Anti-DNase B
In mass spectrometry, the "appearance potential" is defined as the: - ANS-Minimum
energy required for the appearance of a particular fragment ion in mass spectrum
What is generally accepted as the lower threshold value for semen pH from fertile
males? - ANS-7.2
Coulometry: - ANS-An automated method for measuring chloride which generates
silver ions in the reaction
Erythroleukemia: - ANS-Also known as Acute Myelogenous Leukemia type M6. This
type is associated with either the presence of both erythroid and myeloid precursors
OR strictly erythroid precursors.
Myelomonocytic leukemia - ANS-Is associated with an increase in myeloid and
monocytic cell precursors, but not red blood cell precursors
Species that are Lysine (+), arginine (=) and ornithine (+): - ANS-Enterobacter aerogenes,
Edwardsiella species and most Serratia species
Lysine reaction for all Citrobacter: - ANS-Negative
,Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - ANS-negative
reactions for the three decarboxylases commonly tested ( Lysine, arginine and
ornithine)
Which organism is MOST likely responsible for septic reactions associated with Red
Blood Cell transfusions? - ANS-Yersina entercolitica is most likely responsible for
septic reactions in transfusions of Red Blood Cells. This organism is usually acquired
by ingestion of contaminated food and causes mild symptoms of abdominal pain and
diarrhea. Growth of Y. entercolitica is enhanced in iron-rich environments such as red
cell components.
Gaucher's disease - ANS-The most common lysosomal storage disease caused by
insufficient activity of the lysosomal enzyme glucocerebrosidase that leads to the
deposition of glucocerebroside in cells of the macrophage-monocyte system.
Diseases associated with dysfunctions of polymorphonuclear neutrophils (PMNs) -
ANS-Chediak-Higashi syndrome (CH), Chronic granulomatous disease (CGD),
Myeloperoxidase deficiency (MPO)
Nephrotic Syndrome: - ANS-Caused by damage to the kidneys, especially the basement
membrane of the glomerulus; which causes abnormal excretion of protein and red
blood cells in the urine. Fats are also present in the urine in most cases. A foamy
appearance of the urine is a key characteristic of this condition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: - ANS-The key biochemical reactions
by which the family Enterobacteriaceae can be identified include fermentation of
carbohydrates, reduction of nitrates to nitrites and the absence of cytochrome oxidase
activity.
, ANA - ANS-See 2 quiz #27
Prozone effect: - ANS-Results in a false positive reaction; is the result of antibody
excess and dilution of antibody can help prevent its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: - ANS-Minimize the effect
of interference
Key characteristics of Micrococcus: - ANS-Susceptible to bacitracin ("A") and resistant
to furazolidone; gram positive cocci in tetras, bacteria cells of micrococcus luteus are
larger than those of the staphylococci and can arrange in tetras
What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL,
if the urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs? - ANS-95
mL/min
One method of calculating a glomerular filtration rate is using creatinine and urine
volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where
urine flow rate = volume in mL /24 hours x h/60 min)
In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95
ml/min
Estrogen and progesterone markers are most commonly used to provide prognostic
information about: - ANS-Breast cancer
The prozone effect ( when performing a screening titer) is most likely to result in: -
ANS-Prozone effect (due to antibody excess) will result in an initial false negative in
spite of the large amount of antibody in the serum, followed by a positive result as the
specimen is diluted.
Which of the following Rh antigens is found the highest frequency in the Caucasian
population:
Answers Graded A+ 2025-2026
What feature is most often found with patients with a splenectomy on a Wright's
stained blood smear? - ANS-Howell-Jolly bodies
Patients with antibody to the following antigen are immune to Hepatitis B: - ANS-
Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous Streptococcal
throat and skin infections: - ANS-ASO titer and Anti-DNase B
In mass spectrometry, the "appearance potential" is defined as the: - ANS-Minimum
energy required for the appearance of a particular fragment ion in mass spectrum
What is generally accepted as the lower threshold value for semen pH from fertile
males? - ANS-7.2
Coulometry: - ANS-An automated method for measuring chloride which generates
silver ions in the reaction
Erythroleukemia: - ANS-Also known as Acute Myelogenous Leukemia type M6. This
type is associated with either the presence of both erythroid and myeloid precursors
OR strictly erythroid precursors.
Myelomonocytic leukemia - ANS-Is associated with an increase in myeloid and
monocytic cell precursors, but not red blood cell precursors
Species that are Lysine (+), arginine (=) and ornithine (+): - ANS-Enterobacter aerogenes,
Edwardsiella species and most Serratia species
Lysine reaction for all Citrobacter: - ANS-Negative
,Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - ANS-negative
reactions for the three decarboxylases commonly tested ( Lysine, arginine and
ornithine)
Which organism is MOST likely responsible for septic reactions associated with Red
Blood Cell transfusions? - ANS-Yersina entercolitica is most likely responsible for
septic reactions in transfusions of Red Blood Cells. This organism is usually acquired
by ingestion of contaminated food and causes mild symptoms of abdominal pain and
diarrhea. Growth of Y. entercolitica is enhanced in iron-rich environments such as red
cell components.
Gaucher's disease - ANS-The most common lysosomal storage disease caused by
insufficient activity of the lysosomal enzyme glucocerebrosidase that leads to the
deposition of glucocerebroside in cells of the macrophage-monocyte system.
Diseases associated with dysfunctions of polymorphonuclear neutrophils (PMNs) -
ANS-Chediak-Higashi syndrome (CH), Chronic granulomatous disease (CGD),
Myeloperoxidase deficiency (MPO)
Nephrotic Syndrome: - ANS-Caused by damage to the kidneys, especially the basement
membrane of the glomerulus; which causes abnormal excretion of protein and red
blood cells in the urine. Fats are also present in the urine in most cases. A foamy
appearance of the urine is a key characteristic of this condition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: - ANS-The key biochemical reactions
by which the family Enterobacteriaceae can be identified include fermentation of
carbohydrates, reduction of nitrates to nitrites and the absence of cytochrome oxidase
activity.
, ANA - ANS-See 2 quiz #27
Prozone effect: - ANS-Results in a false positive reaction; is the result of antibody
excess and dilution of antibody can help prevent its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: - ANS-Minimize the effect
of interference
Key characteristics of Micrococcus: - ANS-Susceptible to bacitracin ("A") and resistant
to furazolidone; gram positive cocci in tetras, bacteria cells of micrococcus luteus are
larger than those of the staphylococci and can arrange in tetras
What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL,
if the urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs? - ANS-95
mL/min
One method of calculating a glomerular filtration rate is using creatinine and urine
volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where
urine flow rate = volume in mL /24 hours x h/60 min)
In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95
ml/min
Estrogen and progesterone markers are most commonly used to provide prognostic
information about: - ANS-Breast cancer
The prozone effect ( when performing a screening titer) is most likely to result in: -
ANS-Prozone effect (due to antibody excess) will result in an initial false negative in
spite of the large amount of antibody in the serum, followed by a positive result as the
specimen is diluted.
Which of the following Rh antigens is found the highest frequency in the Caucasian
population:
