MMSC 420 Exam 2 Questions and Answers Latest Update 2025-2026
What kind of specimen tube should we use for a DAT? - Answers Purple top, EDTA
Which of the following would NOT be a patient that we would perform a DAT on?
A: A patient with low Hgb and HCT levels but with no known bleeding
B: A mother who has just given birth to a type O neg baby
C: A patient exhibiting signs of an acute hemolytic transfusion reaction after receiving A pos
RBCs from a donor
D: A baby born to a mother who is type O - Answers B
Does a positive DAT result means the patient's RBCs have a decreased lifespan? - Answers no
A: Alloantibodies bound to transfused RBCs
B: Autoantibodies to intrinsic red cell antigens
C: Maternal antibodies that bind to fetal RBCs
ALL cause - Answers positive DAT
How is the alternate pathway of the complement cascade activated? - Answers Liposaccharide
on E. coli binds to C3
A: Newborn with O- mother
B: Recently transfused patient with a positive DAT and a 1+ reaction in all antibody screening
red cells including autocontrol
C: Recently transfused patient with a hemolytic transfusion reaction
ALL indicate - Answers elution needed
How many IgG and IgM, respectively, would be required to activate the classical pathway for the
complement cascade? - Answers 2, 1
What is the order of complement activation in the classical pathway of the complement
cascade? - Answers C1-C4-C2-C3-C5-C9
What test should be done as a reflex after a positive anti-PS DAT is observed in a 27 year old
male? - Answers Both anti-IgG and anti-C3bC3d should be run
What test can be performed to identify ABO antibodies coating a baby's cells? - Answers Lui
Freeze Elution
, What is a distinguishing characteristic in intravascular hemolysis? - Answers Hemoglobinuria
If the DAT is positive but the IAT is negative, what describes the situation accurately? - Answers
There are more RBC antigen sites than antibodies
A pregnant patient has a positive result for DAT anti-PS and positive result for anti-IgG. When
performing an adsorption, what kind of cells do we want to use? - Answers Allogeneic
adsorption
Agglutination with _________________ applies to drug-induced hemolytic anemia caused by
immune complexes - Answers reagent RBCs and drug
What is the most common drug implicated in drug-induced hemolytic anemia caused by
autoimmunity? - Answers Aldomet
Which of the following conditions is not a form of autoimmune hemolytic anemia?
a)Hemolytic disease of fetus & newborn
b)Cold agglutinin disease
c)Paroxysmal cold hemoglobinuria
d)Warm autoimmune hemolytic anemia - Answers A
Cold autoimmune hemolytic anemia is associated with which pathogen? - Answers
Mycoplasma pneumoniae
Rabbit erythrocyte stroma (RESt) is a commercial reagent rich in I-antigen and is used to
facilitate antibody identification with which type of AIHA? - Answers CAD
Which antibody is associated with paroxysmal cold hemoglobinuria (PCH)? - Answers anti-P
Biphasic hemolysin occurs by binding to RBCs at cold temperatures and only causes
complement-mediated lysis when warmed to 37C. Which AIHA is this condition associated with?
- Answers PCH
Does WAIHA involve IgM? - Answers no
Which drug is commonly associated with the drug adsorption mechanism in DIHA? - Answers
Penicillin IV
Cephalosporins are thought to be associated with which DIHA mechanism? - Answers Non-
immunogenic protein adsorption
Anti-CD47 is used to treat organ tumors and other hematologic malignancies by promoting
phagocytosis. What complications can arise from pre-transfusion testing for patients receiving
this treatment? - Answers Positive reactions occur at all phases of testing, including reverse
What kind of specimen tube should we use for a DAT? - Answers Purple top, EDTA
Which of the following would NOT be a patient that we would perform a DAT on?
A: A patient with low Hgb and HCT levels but with no known bleeding
B: A mother who has just given birth to a type O neg baby
C: A patient exhibiting signs of an acute hemolytic transfusion reaction after receiving A pos
RBCs from a donor
D: A baby born to a mother who is type O - Answers B
Does a positive DAT result means the patient's RBCs have a decreased lifespan? - Answers no
A: Alloantibodies bound to transfused RBCs
B: Autoantibodies to intrinsic red cell antigens
C: Maternal antibodies that bind to fetal RBCs
ALL cause - Answers positive DAT
How is the alternate pathway of the complement cascade activated? - Answers Liposaccharide
on E. coli binds to C3
A: Newborn with O- mother
B: Recently transfused patient with a positive DAT and a 1+ reaction in all antibody screening
red cells including autocontrol
C: Recently transfused patient with a hemolytic transfusion reaction
ALL indicate - Answers elution needed
How many IgG and IgM, respectively, would be required to activate the classical pathway for the
complement cascade? - Answers 2, 1
What is the order of complement activation in the classical pathway of the complement
cascade? - Answers C1-C4-C2-C3-C5-C9
What test should be done as a reflex after a positive anti-PS DAT is observed in a 27 year old
male? - Answers Both anti-IgG and anti-C3bC3d should be run
What test can be performed to identify ABO antibodies coating a baby's cells? - Answers Lui
Freeze Elution
, What is a distinguishing characteristic in intravascular hemolysis? - Answers Hemoglobinuria
If the DAT is positive but the IAT is negative, what describes the situation accurately? - Answers
There are more RBC antigen sites than antibodies
A pregnant patient has a positive result for DAT anti-PS and positive result for anti-IgG. When
performing an adsorption, what kind of cells do we want to use? - Answers Allogeneic
adsorption
Agglutination with _________________ applies to drug-induced hemolytic anemia caused by
immune complexes - Answers reagent RBCs and drug
What is the most common drug implicated in drug-induced hemolytic anemia caused by
autoimmunity? - Answers Aldomet
Which of the following conditions is not a form of autoimmune hemolytic anemia?
a)Hemolytic disease of fetus & newborn
b)Cold agglutinin disease
c)Paroxysmal cold hemoglobinuria
d)Warm autoimmune hemolytic anemia - Answers A
Cold autoimmune hemolytic anemia is associated with which pathogen? - Answers
Mycoplasma pneumoniae
Rabbit erythrocyte stroma (RESt) is a commercial reagent rich in I-antigen and is used to
facilitate antibody identification with which type of AIHA? - Answers CAD
Which antibody is associated with paroxysmal cold hemoglobinuria (PCH)? - Answers anti-P
Biphasic hemolysin occurs by binding to RBCs at cold temperatures and only causes
complement-mediated lysis when warmed to 37C. Which AIHA is this condition associated with?
- Answers PCH
Does WAIHA involve IgM? - Answers no
Which drug is commonly associated with the drug adsorption mechanism in DIHA? - Answers
Penicillin IV
Cephalosporins are thought to be associated with which DIHA mechanism? - Answers Non-
immunogenic protein adsorption
Anti-CD47 is used to treat organ tumors and other hematologic malignancies by promoting
phagocytosis. What complications can arise from pre-transfusion testing for patients receiving
this treatment? - Answers Positive reactions occur at all phases of testing, including reverse
