Bio 200 Prelab 2
What molecule is the source of information for transcription? – answer DNA
What is the main enzyme for making a polymer of RNA? – answer RNA polymerase
Rifampicin is an antibiotic that works on / inhibits which of the following in bacteria? -
answerRNA polymerase
Please match the correct answer with each of the following questions. - answer
transcription in prokaryotic cells? - Cytoplasm
translation in eukaryotic cells? - Cytoplasm
translation in prokaryotic cells? - Cytoplasm
Transcription in eukaryotic cells? - Nucleus
In a prokaryotic cell, which 4 of the following events need to happen in order to
recognize where the information for a gene is? [Choose all correct answers.] -
answerTemplate DNA must be present.
Sigma protein must recognize the promoter sequence in the DNA.
The promoter region must include a -10 sequence and a -35 sequence.
Core RNA polymerase enzyme must bind to sigma.
What does PCR do? - answeramplifies DNA - makes many copies of a specific section
of DNA
What is the purpose of 'primers' in the PCR process? - answerThey bind with a specific
region of DNA and bracket the section that will be replicated
Taq polymerase, the blue molecules in the video, what is their purpose? - answerThey
bind to the primer sequences and elongate the new DNA strand
A previous lab ran the PCR for your bacteria. In part two of lab you will be using some
of this product to run a gel electrophoresis. What is the purpose of doing this? -
answerGel electrophoresis separates molecules by size. Based on what we know about
the length of the DNA that codes for cluster I and II, this will let us know if the PCR was
successful and if we should pay to have this PCR product sequenced or not.
The agarose gel should be inserted so that the wells are nearest which electrode? -
answerNegative electrode (Black wires)
What molecule is the source of information for transcription? – answer DNA
What is the main enzyme for making a polymer of RNA? – answer RNA polymerase
Rifampicin is an antibiotic that works on / inhibits which of the following in bacteria? -
answerRNA polymerase
Please match the correct answer with each of the following questions. - answer
transcription in prokaryotic cells? - Cytoplasm
translation in eukaryotic cells? - Cytoplasm
translation in prokaryotic cells? - Cytoplasm
Transcription in eukaryotic cells? - Nucleus
In a prokaryotic cell, which 4 of the following events need to happen in order to
recognize where the information for a gene is? [Choose all correct answers.] -
answerTemplate DNA must be present.
Sigma protein must recognize the promoter sequence in the DNA.
The promoter region must include a -10 sequence and a -35 sequence.
Core RNA polymerase enzyme must bind to sigma.
What does PCR do? - answeramplifies DNA - makes many copies of a specific section
of DNA
What is the purpose of 'primers' in the PCR process? - answerThey bind with a specific
region of DNA and bracket the section that will be replicated
Taq polymerase, the blue molecules in the video, what is their purpose? - answerThey
bind to the primer sequences and elongate the new DNA strand
A previous lab ran the PCR for your bacteria. In part two of lab you will be using some
of this product to run a gel electrophoresis. What is the purpose of doing this? -
answerGel electrophoresis separates molecules by size. Based on what we know about
the length of the DNA that codes for cluster I and II, this will let us know if the PCR was
successful and if we should pay to have this PCR product sequenced or not.
The agarose gel should be inserted so that the wells are nearest which electrode? -
answerNegative electrode (Black wires)
