BIO 305 FINAL EXAM QUESTIONS & ANS..
To create transgenic mice, DNA is injected into Answer - a pronucleus of zygote
How can transgenic organisms be used to identify transcriptional enhancers?
Answer - A reporter gene can be constructed so that its expression is controlled
by an enhancer that may potentially exist within a fragment of genomic DNA
A trait unique to embryonic stem cells that makes them useful for generating
knockout animals is that they Answer - are totipotent
after exposure to Agrobacterium tumefaciens bacteria containing both a
recombinant T-DNA vector and a helper plasmid, new plants can be grown
from single cells in the presence of herbicide. Without the herbicide, Answer -
both transformed and nontransformed plants would grow
A scientist wants to determine whether a mutant phenotype is due to the loss
of gene C or gene D, which are both deleted in a mutant fly with abnormal
eyes. Mutant flies with transgenic gene C have wild-type eyes, whereas mutant
flies with transgenic gene D have abnormal eyes. What can be concluded from
these results? Answer - Gene C is required for normal eye development
The Aniridia gene in humans is involved in eye formation. Although eye
development is very different in flies, this gene is highly conserved. What is the
Drosophila homolog of Aniridia? Answer - eyeless
The presence of a homeodomain in a protein suggests what about its function?
Answer - It is a transcription factor
To create transgenic mice, DNA is injected into Answer - a pronucleus of zygote
How can transgenic organisms be used to identify transcriptional enhancers?
Answer - A reporter gene can be constructed so that its expression is controlled
by an enhancer that may potentially exist within a fragment of genomic DNA
A trait unique to embryonic stem cells that makes them useful for generating
knockout animals is that they Answer - are totipotent
after exposure to Agrobacterium tumefaciens bacteria containing both a
recombinant T-DNA vector and a helper plasmid, new plants can be grown
from single cells in the presence of herbicide. Without the herbicide, Answer -
both transformed and nontransformed plants would grow
A scientist wants to determine whether a mutant phenotype is due to the loss
of gene C or gene D, which are both deleted in a mutant fly with abnormal
eyes. Mutant flies with transgenic gene C have wild-type eyes, whereas mutant
flies with transgenic gene D have abnormal eyes. What can be concluded from
these results? Answer - Gene C is required for normal eye development
The Aniridia gene in humans is involved in eye formation. Although eye
development is very different in flies, this gene is highly conserved. What is the
Drosophila homolog of Aniridia? Answer - eyeless
The presence of a homeodomain in a protein suggests what about its function?
Answer - It is a transcription factor
