1
NBME BIOCHEMISTRY SHELF QUESTIONS
AND VERIFIED ANSWERS (UPDATED
2025/2026)
What is fiber? - correct-answer -glucose beta(1->4) glucose bonded sugar; body
does not have beta(1-4) endoglucosidase
enterokinase - correct-answer -activates trypsinogen; trypsin activates
chymotrypsin, procolipase, procarboxypeptidase, and proelastase
arsenic - correct-answer -pyruvate dehydrogenase ib; smell of garlic
Which GLUT is insulin-dependent? - correct-answer -GLUT4; found in muscle &
adipose
What is the role of F2,6BP? - correct-answer -role player for insulin; increases
glycolysis for FA synth and glycogen synthesis
, 2
What is significant about glucokinase? - correct-answer -K_m is above fasting
concentration, V_max is very high
What promotes lactate buildup? - correct-answer -^ NADH/NAD+
Where does lactate build up? - correct-answer -eyes, testes, RBC's
What is the rate-limiting step of glycolysis? - correct-answer -PFK, allosterically
activated by F2,6BP
Def in pyr kinase leads to... - correct-answer -2,3BPG, G6P elevation due to ^ PEP
What steroid can be used to treat beta-blocker overdose? - correct-answer -
glucagon
Which form of aldolase is liver-specific? - correct-answer -B
, 3
triose phosphate isomerase - correct-answer -G3P <-> DHAP; DHAP - component
of glycerol phosphate shuttle
What TCA step makes GTP? - correct-answer -succinate thiokinase
What TCA step makes FADH? - correct-answer -succinate dehydrogenase
What is the only membrane-bound enzyme in TCA? - correct-answer -succinate
dehydrogenase
What characterizes succinate? - correct-answer -dicarboxylic acid
pyruvate carboxylase - correct-answer -pyr -> OAA; requires biotin
Rotenone - correct-answer -inhibits complex I of ETC
antimycin - correct-answer -ib complex II, succinate dehydrogenase
NBME BIOCHEMISTRY SHELF QUESTIONS
AND VERIFIED ANSWERS (UPDATED
2025/2026)
What is fiber? - correct-answer -glucose beta(1->4) glucose bonded sugar; body
does not have beta(1-4) endoglucosidase
enterokinase - correct-answer -activates trypsinogen; trypsin activates
chymotrypsin, procolipase, procarboxypeptidase, and proelastase
arsenic - correct-answer -pyruvate dehydrogenase ib; smell of garlic
Which GLUT is insulin-dependent? - correct-answer -GLUT4; found in muscle &
adipose
What is the role of F2,6BP? - correct-answer -role player for insulin; increases
glycolysis for FA synth and glycogen synthesis
, 2
What is significant about glucokinase? - correct-answer -K_m is above fasting
concentration, V_max is very high
What promotes lactate buildup? - correct-answer -^ NADH/NAD+
Where does lactate build up? - correct-answer -eyes, testes, RBC's
What is the rate-limiting step of glycolysis? - correct-answer -PFK, allosterically
activated by F2,6BP
Def in pyr kinase leads to... - correct-answer -2,3BPG, G6P elevation due to ^ PEP
What steroid can be used to treat beta-blocker overdose? - correct-answer -
glucagon
Which form of aldolase is liver-specific? - correct-answer -B
, 3
triose phosphate isomerase - correct-answer -G3P <-> DHAP; DHAP - component
of glycerol phosphate shuttle
What TCA step makes GTP? - correct-answer -succinate thiokinase
What TCA step makes FADH? - correct-answer -succinate dehydrogenase
What is the only membrane-bound enzyme in TCA? - correct-answer -succinate
dehydrogenase
What characterizes succinate? - correct-answer -dicarboxylic acid
pyruvate carboxylase - correct-answer -pyr -> OAA; requires biotin
Rotenone - correct-answer -inhibits complex I of ETC
antimycin - correct-answer -ib complex II, succinate dehydrogenase
