Histology ASCP Exam questions with answers

Histology ASCP Exam questions with |\ |\ |\ |\ |\




answers

An example of an additive is one that contains:
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A. Picric acid
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B. Acetic acid
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C. Ethyl alcohol
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D. Acetone - CORRECT ANSWERS ✔✔Picric Acid
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When compared with tissue fixed in formalin, tissue fixed in zinc-
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formalin will show: |\ |\ |\




A. Better ultrastructural preservation
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B. Decreased immunoreactivity
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C. Increased enzyme activity
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D. Superior nuclear detail - CORRECT ANSWERS ✔✔Superior
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nuclear detail |\




Microscopic examination of an H&E stained section fixed in
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formalin shows marked nuclear bubbling. One most often sees
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this artifact if the specimen is processed following:
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A. Incomplete fixation
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B. Prolonged fixation
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C. Microwave fixation
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D. Frozen sectioning - CORRECT ANSWERS ✔✔Incomplete fixation
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,Microscopic evaluation of H&E stained sections from a surgically
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removed small bowel specimen shows an absence of much of the
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epithelium in otherwise normal tissue. This most likely resulted
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from: |\




A. Mechanical trauma
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B. Delayed fixation
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C. Ulceration
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D. Poor choice of fixative - CORRECT ANSWERS ✔✔Delayed
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fixation


A specimen of kidney must be shipped to another city for
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immunofluorescence studies. The specimen should be placed in: |\ |\ |\ |\ |\ |\ |\ |\




A. Saline |\




B. Michel solution
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C. Buffered formalin
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D. Orth solution - CORRECT ANSWERS ✔✔Michel solution
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A certain project requires a fixative that contains acetic acid yet
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stabilizes erythrocyte membranes. One fixative that could be
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used is: |\ |\




A. Zenker solution
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B. Bouin solution
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C. Gendre solution
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D. Hollande solution - CORRECT ANSWERS ✔✔Hollande solution
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,When the microwave oven is used for fixation, the most critical
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factor is the: |\ |\




A. Preparation of the formalin solution
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B. Use of glass containers
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C. Control of the temperature
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D. Osmolality of the fixative solution - CORRECT ANSWERS
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✔✔Control of the temperature |\ |\ |\




To adequately remove the calcium in a specimen containing
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areas of microcalcification, the tissue could be fixed in:
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A. Hollande solution
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B. NBF|\




C. B-5 solution
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D. Zamboni solution - CORRECT ANSWERS ✔✔Hollande solution
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What fixative contains copper acetate?
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A. Hollande
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B. Bouin
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C. Gendre
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D. Zamboni - CORRECT ANSWERS ✔✔Hollande
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A specimen is submitted with the statement that it was fixed in
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formalin. Microscopic sections show marked lysis of erythrocytes.
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This indicates that the fixative most likely was:
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A. Prepared with too much formalin
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B. Buffered above neutrality
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, C. Acidified with acetic acid
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D. Not formalin - CORRECT ANSWERS ✔✔Acidified with acetic
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acid


Fixatives are classified as additive because of the:
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A. Addition of several chemicals to the solution
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B. Addition, or binding of the fixative to tissue proteins
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C. Additional reactions occurring with longer fixation
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D. Additional reactive tissue sites available for dye binding -
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CORRECT ANSWERS ✔✔Addition, or binding of the fixative to
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tissue proteins
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Kidney biopsy tissue has been fixed in phosphate-buffered
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glutaraldehyde for 2 hours and then placed in phosphate buffer |\ |\ |\ |\ |\ |\ |\ |\ |\ |\


solution. If a portion of this tissue is processed for light
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microscopy, sections would most likely show: |\ |\ |\ |\ |\




A. Very poor glomerular preservation
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B. Decreased uptake of hematoxylin
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C. Lysis of cytoplasmic elements
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D. Nonspecific PAS staining - CORRECT ANSWERS ✔✔Nonspecific
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PAS staining
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Uric acid crystals are preserved ONLY when tissue is fixed in:
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A. Absolute alcohol
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B. NBF
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C. Orth solution
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