Please check the examination details below before entering your
candidate information
Candidate surnameOther names
Centre Number Candidate Number
Pearson Edexcel Level
3 GCE
Monday 12 May
Morning (Time: 1 hour 30 minutes)
2025 referen 8BI0/ 🟐 🟐
ce
Biology B
Advanced Subsidiary Paper 01
PAPER 1: Core Cellular Biology and Microbiology
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Pearson Edexcel Level 3 GCE Biology B Advanced Subsidiary PAPER 1: Core Cellular Biology and Microbiology
QP MAY 2025
Instructions
• Use black ink or ball-point pen.
• Fill in the boxes at the top of this page with your
centre number and candidate number.
•
• Answer
name, all
Answer the questions in the spaces
provided – there may be more space
questions.
than you need.
Information
• The total mark for this paper is 80.
• The marks for each question are shown in
– use this as a guide as to how much time to spend on each question.
• In question(s) marked with an asterisk (*), marks will be awarded for
brackets
your ability to structure your answer logically, showing how the
points that you make are related or follow on from each other where
appropriate.
Advice
• Read each question carefully before you start to answer it.
• Try to answer every
• Check your answers if you have time at the end.
question.
Turn over
P78689A
©2025 Pearson
,Education Ltd.
Y:1/1/1/1/1/1/1/
, Some questions must be answered with a cross in a box . If
you change your mind about an answer, put a line through the box and
then mark your new answer with a cross .
AREA
DO NOT WRITE IN THIS
1 Some carbohydrates are composed of three monosaccharides.
These are called trisaccharides.
Raffinose is a trisaccharide composed of glucose, galactose and fructose.
(a) Complete the diagram to show the structure of an alpha glucose molecule.
(2)
CH2OH
C O
H
C C
C C
AREA
DO NOT WRITE IN THIS
(b) Name the bond that joins monosaccharides
together. (1)
............. ............ .......... ........... ........... ........... ........... ........... ............ .......... ........... .... ............ ........... ........... ........... ........... ........... ........... ........... ........... ........... ........... ..... ......................
(c) The three monosaccharides present in raffinose each have a
molecular mass of 180.
The relative atomic mass of the element hydrogen is 1 and the relative
atomic mass of the element oxygen is 16.
Calculate the molecular mass of raffinose.
AREA
DO NOT WRITE IN THIS
(1)
Answer ..............................................................
(Total for Question 1 = 4 marks)
2
■■■
■
, 2 During the S phase of the cell cycle, DNA is
synthesised by semi-conservative replication.
The diagram shows part of a DNA molecule.
DO NOT WRITE IN THIS
bases J and K
AREA
bases L and M
bond E
DO NOT WRITE IN THIS
bond D bond F
(a) (i) Name the base pairs labelled J and K, L
and M. (2)
AREA
J and K . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
L and M ............................................................................................................................... ..............................................................................................................................
(1) Name the bonds labelled D, E and F.
(2)
Bond D . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Bond E . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..
Bond F . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..
DO NOT WRITE IN THIS
AREA
3
■■■ Turn
■ over
candidate information
Candidate surnameOther names
Centre Number Candidate Number
Pearson Edexcel Level
3 GCE
Monday 12 May
Morning (Time: 1 hour 30 minutes)
2025 referen 8BI0/ 🟐 🟐
ce
Biology B
Advanced Subsidiary Paper 01
PAPER 1: Core Cellular Biology and Microbiology
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Pearson Edexcel Level 3 GCE Biology B Advanced Subsidiary PAPER 1: Core Cellular Biology and Microbiology
QP MAY 2025
Instructions
• Use black ink or ball-point pen.
• Fill in the boxes at the top of this page with your
centre number and candidate number.
•
• Answer
name, all
Answer the questions in the spaces
provided – there may be more space
questions.
than you need.
Information
• The total mark for this paper is 80.
• The marks for each question are shown in
– use this as a guide as to how much time to spend on each question.
• In question(s) marked with an asterisk (*), marks will be awarded for
brackets
your ability to structure your answer logically, showing how the
points that you make are related or follow on from each other where
appropriate.
Advice
• Read each question carefully before you start to answer it.
• Try to answer every
• Check your answers if you have time at the end.
question.
Turn over
P78689A
©2025 Pearson
,Education Ltd.
Y:1/1/1/1/1/1/1/
, Some questions must be answered with a cross in a box . If
you change your mind about an answer, put a line through the box and
then mark your new answer with a cross .
AREA
DO NOT WRITE IN THIS
1 Some carbohydrates are composed of three monosaccharides.
These are called trisaccharides.
Raffinose is a trisaccharide composed of glucose, galactose and fructose.
(a) Complete the diagram to show the structure of an alpha glucose molecule.
(2)
CH2OH
C O
H
C C
C C
AREA
DO NOT WRITE IN THIS
(b) Name the bond that joins monosaccharides
together. (1)
............. ............ .......... ........... ........... ........... ........... ........... ............ .......... ........... .... ............ ........... ........... ........... ........... ........... ........... ........... ........... ........... ........... ..... ......................
(c) The three monosaccharides present in raffinose each have a
molecular mass of 180.
The relative atomic mass of the element hydrogen is 1 and the relative
atomic mass of the element oxygen is 16.
Calculate the molecular mass of raffinose.
AREA
DO NOT WRITE IN THIS
(1)
Answer ..............................................................
(Total for Question 1 = 4 marks)
2
■■■
■
, 2 During the S phase of the cell cycle, DNA is
synthesised by semi-conservative replication.
The diagram shows part of a DNA molecule.
DO NOT WRITE IN THIS
bases J and K
AREA
bases L and M
bond E
DO NOT WRITE IN THIS
bond D bond F
(a) (i) Name the base pairs labelled J and K, L
and M. (2)
AREA
J and K . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
L and M ............................................................................................................................... ..............................................................................................................................
(1) Name the bonds labelled D, E and F.
(2)
Bond D . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Bond E . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..
Bond F . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..
DO NOT WRITE IN THIS
AREA
3
■■■ Turn
■ over
