BIOL 3201 Unit 1 Test Questions Answered Correctly Latest Update

BIOL 3201 Unit 1 Test Questions Answered Correctly Latest Update BSL 1 - Answers Microorganisms not known to cause disease in healthy adults. BSL 2 - Answers Indigenous microorganisms that can lead to diseases of varying severity in healthy adults BSL 3 - Answers Indigenous or exotic microorganisms that cause serious or potentially lethal disease through respiratory transmission BSL 4 - Answers Microorganisms that are dangerous and exotic with high risk of aerosol transmitted infections. Rarely are there treatments or vaccines for these microorganisms, and the diseases they cause are frequently fatal Angelina Heese - Answers successful integration of agar into Koch's laboratory Koch - Answers designed a glass dish which could hold gelatin and agar for microbial growth Petri - Answers modifications to Koch's dish 10x - Answers low power 40x - Answers high dry 100x - Answers oil immersion, 1000x total to obtain the correct staining results, how old does gram positive bacteria need to be? - Answers 18-24 hr gram stain - Answers crystal violet, iodine, ethanol (decolorization), safranin (pink) catalase test - Answers Differentiates microorganisms based on their ability to produce the catalase enzyme. Catalase breaks hydrogen peroxide down to water and oxygen. (Caution: Too much hydrogen peroxide will "water down" the reaction and give a false negative result.) aerotolerant bacteria lack catalase pos neg - Answers pos - bubbles neg- none oxidase test - Answers Differentiates microorganisms based on the presence of the enzyme cytochrome C oxidase, producing oxidized cytochrome C and water, 30 sec cut off oxidase pos neg - Answers blue = pos none/yellow = neg KOH test - Answers Confirms Gram stain based on whether 3% KOH can lyse bacterial cell wall and release DNA, sample must be younger than 24 hr, lyses cells in the KOH solution, releasing their DNA and causing the liquid to become very viscous. The KOH test IS NOT a replacement for the Gram stain technique. KOH pos neg - Answers strings = KOH pos = gram neg none = KOH neg = gram pos selective medium - Answers media that will allow only certain microorganisms to grow and will inhibit the growth of other microorganisms, MSA differential medium - Answers contains substances that cause some bacteria to take on an appearance that distinguishes them from other bacteria, SBA aerobic bacteria - Answers When they grow by respiration, they use oxygen as a terminal electron acceptor, converting it to water. However, they also produce hydrogen peroxide and then produce the enzyme catalase, which degrades hydrogen peroxide into harmless oxygen and water