BIOL 3201 final Test Questions Answered Correctly Latest Update 2025-2026
BSL 1 - Answers *Microorganisms not known to cause disease in healthy adults.
*Examples: Bacillus subtilis, Serratia marcescens, Staphylococcus saprophyticus
-Standard microbiological practices are followed
-Personal protective equipment (PPE) is not required.
-A sink must be available for hand washing.
BSL 2 - Answers *Indigenous microorganisms that can lead to diseases of varying severity in
healthy adults.
*Examples: Staphylococcus aureus, Streptococcus pyogenes, Salmonella
-Laboratory access is restricted.
-PPE must be worn including lab coats and gloves.
-Eye protection and face shields are worn when needed.Work that may generate aerosols or
splashes are performed in a biological safety cabinet.
-Contaminated waste is autoclaved.
-The laboratory has self-closing doors. A sink and eyewash are available.
BSL 3 - Answers *Indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission.
*Examples: Mycobacterium tuberculosis, Bacillus anthracis, Francisella tularensis
-Medical surveillance of laboratory employees. Immunizations may be required.
-Locking, double door access separates the lab's work area from other parts of the facility.
-PPE must be worn including lab coats, gloves, eye protection, and respirators.
-All work with microorganisms must be performed in a biosafety cabinet.
-Decontamination of all waste and lab clothing before laundering.
, -Laboratory must have negative air-flow, and exhaust air cannot be recirculated.
-A hands-free sink and eyewash must be available.
BSL 4 - Answers *Microorganisms that are dangerous and exotic with high risk of aerosol
transmitted infections. Rarely are there treatments or vaccines for these microorganisms, and
the diseases they cause are frequently fatal.
*Examples: Ebola virus, Marburg virus
-Change clothing before entering lab.
-Shower upon exiting lab
-Decontaminate all materials before exiting lab.
-All work with microorganisms must be performed in a ClassIII biosafety cabinet or by wearing a
full body, positive pressure suit
-Laboratory must have negative air-flow, and exhaust air cannot be recirculated.
-The laboratory must be in a separate building or in an isolated and restricted zone of the
building.
microorganism - Answers a general term for living organisms that cannot be seen without a
microscope, even though some people include viruses and prions in the group.
"Ubiquity of Microorganisms" - Answers refers to the concept that microorganisms are
everywhere.
disinfectants - Answers can destroy vegetative cells and viruses but may not destroy
endospores
Brightfield microscopes - Answers -simplest form of microscopy where light is either passed
through, or reflected off, a specimen.
-Illumination is not altered by devices that changethe properties of light (such as polarizers or
filters).
-Requires the use of stains to visualize cells.
Phase contrast microscopes - Answers -converts the differences in optical density (refractive
index) of cells into different shades of brightness
-Allows for the visualization of morphology, external structures, and some internal structures.
BSL 1 - Answers *Microorganisms not known to cause disease in healthy adults.
*Examples: Bacillus subtilis, Serratia marcescens, Staphylococcus saprophyticus
-Standard microbiological practices are followed
-Personal protective equipment (PPE) is not required.
-A sink must be available for hand washing.
BSL 2 - Answers *Indigenous microorganisms that can lead to diseases of varying severity in
healthy adults.
*Examples: Staphylococcus aureus, Streptococcus pyogenes, Salmonella
-Laboratory access is restricted.
-PPE must be worn including lab coats and gloves.
-Eye protection and face shields are worn when needed.Work that may generate aerosols or
splashes are performed in a biological safety cabinet.
-Contaminated waste is autoclaved.
-The laboratory has self-closing doors. A sink and eyewash are available.
BSL 3 - Answers *Indigenous or exotic microorganisms that cause serious or potentially lethal
disease through respiratory transmission.
*Examples: Mycobacterium tuberculosis, Bacillus anthracis, Francisella tularensis
-Medical surveillance of laboratory employees. Immunizations may be required.
-Locking, double door access separates the lab's work area from other parts of the facility.
-PPE must be worn including lab coats, gloves, eye protection, and respirators.
-All work with microorganisms must be performed in a biosafety cabinet.
-Decontamination of all waste and lab clothing before laundering.
, -Laboratory must have negative air-flow, and exhaust air cannot be recirculated.
-A hands-free sink and eyewash must be available.
BSL 4 - Answers *Microorganisms that are dangerous and exotic with high risk of aerosol
transmitted infections. Rarely are there treatments or vaccines for these microorganisms, and
the diseases they cause are frequently fatal.
*Examples: Ebola virus, Marburg virus
-Change clothing before entering lab.
-Shower upon exiting lab
-Decontaminate all materials before exiting lab.
-All work with microorganisms must be performed in a ClassIII biosafety cabinet or by wearing a
full body, positive pressure suit
-Laboratory must have negative air-flow, and exhaust air cannot be recirculated.
-The laboratory must be in a separate building or in an isolated and restricted zone of the
building.
microorganism - Answers a general term for living organisms that cannot be seen without a
microscope, even though some people include viruses and prions in the group.
"Ubiquity of Microorganisms" - Answers refers to the concept that microorganisms are
everywhere.
disinfectants - Answers can destroy vegetative cells and viruses but may not destroy
endospores
Brightfield microscopes - Answers -simplest form of microscopy where light is either passed
through, or reflected off, a specimen.
-Illumination is not altered by devices that changethe properties of light (such as polarizers or
filters).
-Requires the use of stains to visualize cells.
Phase contrast microscopes - Answers -converts the differences in optical density (refractive
index) of cells into different shades of brightness
-Allows for the visualization of morphology, external structures, and some internal structures.
