ASCP CG Certification Practice 2025 UPDATE LATEST EXAM
VERSION MOST TESTED QUESTIONS WITH VERIFIED
ANSWERS GRADED A+ ASSURED SUCCESS
What is the best agent to use against mycoplasma?: BM cyclin - a
1.
combination of two antibiotics (tiamulin and minocycline) that ettecticely targets
mycoplasma growth
For successful harvest, each new batch of hypotonic solution
2.
should be eval- uated by:: comparing the new lot to a lot already in use
3.Colagenase, pronase, and trypsin are:: Proteolytic enzymes - break down
tissues into single-cell suspensions
4.When harvesting peripheral blood cultures, asceptic
technique no longer matters following incubation with:: Colcemid -
mitotic spindle poison that halts cells at metaphase
5. Samples that arrive at the cytogenetics lab should first be:: Logge
in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into
6.
division?: T-cell lymphocytes
7.To avoid culture failure, monitoring reagents is crucial. When
logging a cul- ture failure, what is the MOST important to
document?: Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS
8.
(chorionic villus) sample?: Dissection
, Which cell type is most problematic if found in a CVS
9.
(chorionic villus) sam- ple?: Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a
10.
lot of cytoplasm is still seen around the mets, what is the most likely
cause?: innappropriate slide drying time
What will help prevent microbial contamination and cross
11.
contamination of cultures?: Asceptic technique
When there are not enough mets to complete the study of a
12.
patient/case, what needs to be done next?: prepare additional slides from
any remaining sample
When looking at a dropped slide under a phase scope and
13.
mets are very spread out and sparse, what could help fix the issue?:
Increading the drying time by using warm slides
14. What is true of an inverted microscope?: the objectives are beneath the
stage
15.High-resolution banding is indicated for a possible diagnosis
of:: digenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
,"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide
spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic
16.
analysis, what is the MOST important data to accompany the
specimen?: Reason for referral
17.Which type of specimen should be requested for a
cytogenetic analysis for turner syndrome?: Heparinized peripheral blood
stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a
18.
malignancy only if:: Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects
19.
of the incubator are important to monitor to assess the source
of the problem?: Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of
20.
chromosomes viewed under a phase contrast scope is:: Slide drying
, after the cells have been dropped
What is the modal number (default number of chromosomes
21.
in a cell) for a Down syndrome translocation carrier?: 45
What is the best tissue to use for both a good success rate
22.
and abnormality detection in spontaneous abortion miscarriages?:
Chorionic villi
What is a possible cause for a drying oven to not maintain a
23.
proper temper- ature?: The door does not seal properly
If a tech with a cold and sneezing does not take proper
24.
precautions while setting up amniotic fluid samples, the
specimen could become contaminated with:: Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific stain us
on the sample mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
25. What about the metaphases on a slide are affected by slide
making?: Cell density
- thru dilution of the sample with fix
VERSION MOST TESTED QUESTIONS WITH VERIFIED
ANSWERS GRADED A+ ASSURED SUCCESS
What is the best agent to use against mycoplasma?: BM cyclin - a
1.
combination of two antibiotics (tiamulin and minocycline) that ettecticely targets
mycoplasma growth
For successful harvest, each new batch of hypotonic solution
2.
should be eval- uated by:: comparing the new lot to a lot already in use
3.Colagenase, pronase, and trypsin are:: Proteolytic enzymes - break down
tissues into single-cell suspensions
4.When harvesting peripheral blood cultures, asceptic
technique no longer matters following incubation with:: Colcemid -
mitotic spindle poison that halts cells at metaphase
5. Samples that arrive at the cytogenetics lab should first be:: Logge
in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into
6.
division?: T-cell lymphocytes
7.To avoid culture failure, monitoring reagents is crucial. When
logging a cul- ture failure, what is the MOST important to
document?: Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS
8.
(chorionic villus) sample?: Dissection
, Which cell type is most problematic if found in a CVS
9.
(chorionic villus) sam- ple?: Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a
10.
lot of cytoplasm is still seen around the mets, what is the most likely
cause?: innappropriate slide drying time
What will help prevent microbial contamination and cross
11.
contamination of cultures?: Asceptic technique
When there are not enough mets to complete the study of a
12.
patient/case, what needs to be done next?: prepare additional slides from
any remaining sample
When looking at a dropped slide under a phase scope and
13.
mets are very spread out and sparse, what could help fix the issue?:
Increading the drying time by using warm slides
14. What is true of an inverted microscope?: the objectives are beneath the
stage
15.High-resolution banding is indicated for a possible diagnosis
of:: digenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
,"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide
spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic
16.
analysis, what is the MOST important data to accompany the
specimen?: Reason for referral
17.Which type of specimen should be requested for a
cytogenetic analysis for turner syndrome?: Heparinized peripheral blood
stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a
18.
malignancy only if:: Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects
19.
of the incubator are important to monitor to assess the source
of the problem?: Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of
20.
chromosomes viewed under a phase contrast scope is:: Slide drying
, after the cells have been dropped
What is the modal number (default number of chromosomes
21.
in a cell) for a Down syndrome translocation carrier?: 45
What is the best tissue to use for both a good success rate
22.
and abnormality detection in spontaneous abortion miscarriages?:
Chorionic villi
What is a possible cause for a drying oven to not maintain a
23.
proper temper- ature?: The door does not seal properly
If a tech with a cold and sneezing does not take proper
24.
precautions while setting up amniotic fluid samples, the
specimen could become contaminated with:: Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a specific stain us
on the sample mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
25. What about the metaphases on a slide are affected by slide
making?: Cell density
- thru dilution of the sample with fix
