Clinical Chemistry I ASCP review (youlazy) UPDATED ACTUAL
Questions and CORRECT Answers
1. Which of the following lamps provides a continu- A most common light source
ous spectrum of radiant energy in the visible, near for photometry in the visible
IR, and near UV regions of the spectrum? region. continuous spectrum
A. Tungsten-filament (360-800 nm). near IR and UV.
B. Hydrogen
C. Deuterium Hydrogen and Deuterium
D. Mercury vapor (200-375 nm)
Mercury vapor- not a continu-
ous spectrum, emitting radia-
tion at specific wavelengths
2. Which of the following isolates light B Use for selecting a nar-
within a narrow region of the spectrum? row band wavelengths from
A. Photomultiplier tube a continuous spectrum. Three
B. Monochromator kinds (prisms, filters, diffrac-
C. Photovoltaic cell tion gratings.
D. Detector
3. Which of the following is not descriptive of a pho- C
tomultiplier tube? PMT- responds to the radi-
A. Emits electrons proportionally to ant energy light it absorbs by
initial light absorbed emitting electrons in a propor-
B. Must be shielded from stray light tional amount to the initial light
C. Cannot be used with a chopper absorbed.
D. Amplifies the initial signal received
4. Which of the following is false about D is part of a barrier layer cell
a photomultiplier tube? PMT- 1. light to electricity
A. Converts radiant energy (light) 2. Amplification
to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
, Clinical Chemistry I ASCP review (youlazy) UPDATED ACTUAL
Questions and CORRECT Answers
D. Is composed of an iron plate and
a layer of selenium
5. 5. Which type of photodetector employs a linear D
arrangement that allows it to respond to a specific 256-2048 arranged in a linear
wavelength resulting in complete UV/visible spec- fashion
trum analysis? Respond to a specific wave-
A. Photomultiplier tube length that results in a contin-
B. Phototube uous UV/ visible spectrum.
C. Barrier layer cell
D. Photodiode array
6. When performing spectrophotometer D
quality assurance checks, what is the
holmium oxide glass filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
D. Wavelength accuracy
7. In spectrophotometric analysis, what is the pur- D
pose of the reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents
8. In regard to bichromatic analysis, which of the fol- A
lowing is false?
A. Absorbance is measured at the
spectral absorbance peak for a blank
and the sample using the same
wavelength.
, Clinical Chemistry I ASCP review (youlazy) UPDATED ACTUAL
Questions and CORRECT Answers
B. Eliminates background interferences
C. Sample concentration determined
from difference in two measured
absorbances
D. Functions as a reference blank for
each sample
9. The bandpass of a spectrophotometer is C
10 nm. If an instalment is set at 540 nm, the wave- 540 +/- 5nm (10nm)
lengths that are permitted to impinge on the sam-
ple will be within what
wavelength range?
A. 530-540 nm
B. 530-550 nm
C. 535-545 nm
D. 540-550 nm
10. Which of the following formulas is an A
expression of the Beer-Lambert law that is
routinely applied to spectrophotometric
analysis?
A. A u X C s/As = C u
B. C u X C s/As= A u
C. A s X C s/C u = A u
D. A = 2 - log %T
11. 1 1 . In spectrophotometry, which of the following C
is a mathematical expression of the relationship
between absorbance and transmittance?
A. A — abc
R A u/ C u = A s/C s
, Clinical Chemistry I ASCP review (youlazy) UPDATED ACTUAL
Questions and CORRECT Answers
C. A = 2 - log %T
D. A = log %T
12. Which of the following is not a problem inherent in D
turbidimetry?
A. Variation in particle size of samples
B. Variation in particle size of standards
C. Rate of aggregation or settling of
particles
D. Need to maintain a constant and
specific temperature
13. Which of the following may be asso- C
ciated with reflectance spectrophotometry as it re-
lates to the dry reagent slide technique?
A. Light projected to the slide at
180-degree angle
B. Dye concentration directly
proportional to reflectance
C. Unabsorbed, reflected light detected
by photodetector
D. Reflectance values are linearly
proportional to transmission values
14. Fluorometers are designed so that the path of the D
exciting light is at a right angle to the path of the
emitted light. What is the purpose of this design?
A. Prevent loss of emitted light
B. Prevent loss of the excitation light
C. Focus emitted and excitation light
upon the detector
Questions and CORRECT Answers
1. Which of the following lamps provides a continu- A most common light source
ous spectrum of radiant energy in the visible, near for photometry in the visible
IR, and near UV regions of the spectrum? region. continuous spectrum
A. Tungsten-filament (360-800 nm). near IR and UV.
B. Hydrogen
C. Deuterium Hydrogen and Deuterium
D. Mercury vapor (200-375 nm)
Mercury vapor- not a continu-
ous spectrum, emitting radia-
tion at specific wavelengths
2. Which of the following isolates light B Use for selecting a nar-
within a narrow region of the spectrum? row band wavelengths from
A. Photomultiplier tube a continuous spectrum. Three
B. Monochromator kinds (prisms, filters, diffrac-
C. Photovoltaic cell tion gratings.
D. Detector
3. Which of the following is not descriptive of a pho- C
tomultiplier tube? PMT- responds to the radi-
A. Emits electrons proportionally to ant energy light it absorbs by
initial light absorbed emitting electrons in a propor-
B. Must be shielded from stray light tional amount to the initial light
C. Cannot be used with a chopper absorbed.
D. Amplifies the initial signal received
4. Which of the following is false about D is part of a barrier layer cell
a photomultiplier tube? PMT- 1. light to electricity
A. Converts radiant energy (light) 2. Amplification
to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
, Clinical Chemistry I ASCP review (youlazy) UPDATED ACTUAL
Questions and CORRECT Answers
D. Is composed of an iron plate and
a layer of selenium
5. 5. Which type of photodetector employs a linear D
arrangement that allows it to respond to a specific 256-2048 arranged in a linear
wavelength resulting in complete UV/visible spec- fashion
trum analysis? Respond to a specific wave-
A. Photomultiplier tube length that results in a contin-
B. Phototube uous UV/ visible spectrum.
C. Barrier layer cell
D. Photodiode array
6. When performing spectrophotometer D
quality assurance checks, what is the
holmium oxide glass filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
D. Wavelength accuracy
7. In spectrophotometric analysis, what is the pur- D
pose of the reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents
8. In regard to bichromatic analysis, which of the fol- A
lowing is false?
A. Absorbance is measured at the
spectral absorbance peak for a blank
and the sample using the same
wavelength.
, Clinical Chemistry I ASCP review (youlazy) UPDATED ACTUAL
Questions and CORRECT Answers
B. Eliminates background interferences
C. Sample concentration determined
from difference in two measured
absorbances
D. Functions as a reference blank for
each sample
9. The bandpass of a spectrophotometer is C
10 nm. If an instalment is set at 540 nm, the wave- 540 +/- 5nm (10nm)
lengths that are permitted to impinge on the sam-
ple will be within what
wavelength range?
A. 530-540 nm
B. 530-550 nm
C. 535-545 nm
D. 540-550 nm
10. Which of the following formulas is an A
expression of the Beer-Lambert law that is
routinely applied to spectrophotometric
analysis?
A. A u X C s/As = C u
B. C u X C s/As= A u
C. A s X C s/C u = A u
D. A = 2 - log %T
11. 1 1 . In spectrophotometry, which of the following C
is a mathematical expression of the relationship
between absorbance and transmittance?
A. A — abc
R A u/ C u = A s/C s
, Clinical Chemistry I ASCP review (youlazy) UPDATED ACTUAL
Questions and CORRECT Answers
C. A = 2 - log %T
D. A = log %T
12. Which of the following is not a problem inherent in D
turbidimetry?
A. Variation in particle size of samples
B. Variation in particle size of standards
C. Rate of aggregation or settling of
particles
D. Need to maintain a constant and
specific temperature
13. Which of the following may be asso- C
ciated with reflectance spectrophotometry as it re-
lates to the dry reagent slide technique?
A. Light projected to the slide at
180-degree angle
B. Dye concentration directly
proportional to reflectance
C. Unabsorbed, reflected light detected
by photodetector
D. Reflectance values are linearly
proportional to transmission values
14. Fluorometers are designed so that the path of the D
exciting light is at a right angle to the path of the
emitted light. What is the purpose of this design?
A. Prevent loss of emitted light
B. Prevent loss of the excitation light
C. Focus emitted and excitation light
upon the detector
