ANSWERS|ACTUAL COMPLETE EXAM|ALREADY GRADED A+
1. Which immunoassay methodology is a. Chemiluminescence
the emission of light by molecules in
an excited state with a limited amount Chemiluminescence is the emission of
light by
of heat as the result of a molecules in an excited state with a limited
chemical re- action? amount of heat (luminescence) as the result of
Question a chemical reaction. Chemiluminescent assays
are ultrasensi- tive and are widely used in
a.Chemiluminescence automated immunoas- says and DNA probe
b.Immunochromatography assay systems. Chemilumi- nescence has
c.Photometry excellent sensitivity and dynamic range.
d.Spectrophotometry Chemiluminescent labels can be attached
to an antigen or an antibody. Most
chemilumi- nescent reagents and
conjugates are stable and relatively
nontoxic.
2. What characteristics/functions do cal- a. Contains a known amount of analyte
being test-
ibrators have? calculation?
Question
a.2 - log10 %T
a. Contains a known amount b.2 +
of ana- lyte being tested log10
b. Monitors the quality of %T
reagents c.log1
c. Monitors the quality of the 0 %T -
sample 2
d. Prevents equipment failure d.log1
0 %T
3. Absorbance can be calculated
+2
easi- ly from percent
transmission using which
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d
alibrators are used to establish a correlation be- tween
the measurement response and the con- centration
or amount of the substance that is being measured by
the test procedure.
2 - log10 %T
sorbance can be calculated easily from percent transmission
using 2 - log10 %T. If all the light passes through a
solution without any absorption, absorbance is zero
and the %T is 100%. If all the light is absorbed, %T is
zero, and absorption is infinite.
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4. What is a substance or c. An analyte
constituent in which the
laboratory conducts test- ing? In the testing process, analytes or constituents
are measured.
a.A solution
b. An additive
c. An analyte
d. An anticoagulant
5. What does a common d. study crystals in urine and cells
application of the polarized
light microscope in the clinical
laboratory do?
Determine FANAs with
fluorescence b.Determine
neutrophilic from lym-
phocytic leukemia
c.Examine histochemical
reactions
d.Study crystals in urine and in
cells
6. What is the chelation of calcium in the b. Prevents coagulation by inhibiting
coagulation
EDTA tube?
d. Stops the lysing of
a. Maintains a constant the red blood cells
temperature for components
b. Prevents coagulation by 7. How must a laboratory
inhibiting coagulation operate to handle TB
c. Prevents the separation of sputum and TM
blood components materials?
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ANSWERS|ACTUAL COMPLETE EXAM|ALREADY GRADED A+
CLSI recommends the salts of the chelating (cal- cium
binding) agent EDTA as the anticoagulant of choice for
blood cell counting and sizing.
a. Biosafety level of 2+ or 3