AMT MLS MICROBIOLOGY EXAM WITH ALL CORRECT &
100% VERIFIED ANSWERS|ACTUAL COMPLETE EXAM|
ALREADY GRADED A+
what are the three bacterial morphologies? ✔Correct Answer-cocci
bacilli
spirochetes
what are the shapes of cocci bacteria? ✔Correct Answer-sphere-shaped
single cells
pairs
clustes
tetrads
chains
what are the shapes of bacilli? ✔Correct Answer-rods
single cells
chains
palisades
coccobilli
fusiform
what shape are spirochete bacteria? ✔Correct Answer-spiral shaped
what bacteria require an environment with O2? ✔Correct Answer-aerobic bacteria
what bacteria do not need O2 to grow and reproduce? ✔Correct Answer-anaerobic bacteria
what bacteria are capable of growing with or without bacteria? ✔Correct Answer-facultative
aerobic bacteria
what is a facultative anaerobic bacteria? ✔Correct Answer-a bacteria that grows best with oxygen
but can grow with little to no oxygen
what is a microaerophillic bacteria? ✔Correct Answer-a bacteria that grows better with lower O2
and higher CO2
what is a capnophillic bacteria? ✔Correct Answer-A bacteria that grows best in environments with
high CO2
what is a mesophilic bacteria? ✔Correct Answer-a bacteria that grows best in moderate
temperatures 25C-40C
what is a thermophilic bacteria? ✔Correct Answer-a bacteria that grows best at high temps
typically 50C-60C
what is an autotrophic bacteria? ✔Correct Answer-a bacteria that uses light or inorganic
substances to synthesize their food or energy
, what is a heterotrophic bacteria? ✔Correct Answer-a bacteria that organic material as a source of
food and energy or more specifically carbon
what are the three types of bacterial stains? ✔Correct Answer-gram
acid fast
fluorescent
what type of stain is used most in clinical microbiology? ✔Correct Answer-the gram stain
what color is gram positive? ✔Correct Answer-purple remember positive equals purple
what color is gram negative? ✔Correct Answer-pink reddish
what kind of bacteria are acid fast stains used for? ✔Correct Answer-high lipid and wax content in
the cell wall
what is the primary stain used in acid fast? ✔Correct Answer-carbol fuchsin
what color do acid fast bacteria stain? ✔Correct Answer-red
what color do non acid fast bacteria stain? ✔Correct Answer-blue
when is acridine orange fluorescent stain used? ✔Correct Answer-when bacteria are difficult to
find using other stain techniques
when is calcofluor white fluorescent stain used? ✔Correct Answer-to identify fungal structures
what is the gram stain procedure? ✔Correct Answer-1. place smears and controls on a staining
rack over the sink.
2. flood with crystal violet for 30 seconds
3. rinse with water
4. flood with grams iodine for 30-60 secs
5. rinse with water
6. decolorize with alcohol-acetone until it runs clear then rinse with water
7. flood with safranin for 30-60 secs
8. rinse with water and dry
to remember what chemicals go first just go in alphabetical order
C(rystal violet)
G(rams iodine)
S(afranin)
what are inhibitors? ✔Correct Answer-additives that prevent the growth of a specific type of
bacteria making it easier to identify other bacterias
ex. Columbia agar containing nalidixic acid that inhibits non gram positive bacteria
100% VERIFIED ANSWERS|ACTUAL COMPLETE EXAM|
ALREADY GRADED A+
what are the three bacterial morphologies? ✔Correct Answer-cocci
bacilli
spirochetes
what are the shapes of cocci bacteria? ✔Correct Answer-sphere-shaped
single cells
pairs
clustes
tetrads
chains
what are the shapes of bacilli? ✔Correct Answer-rods
single cells
chains
palisades
coccobilli
fusiform
what shape are spirochete bacteria? ✔Correct Answer-spiral shaped
what bacteria require an environment with O2? ✔Correct Answer-aerobic bacteria
what bacteria do not need O2 to grow and reproduce? ✔Correct Answer-anaerobic bacteria
what bacteria are capable of growing with or without bacteria? ✔Correct Answer-facultative
aerobic bacteria
what is a facultative anaerobic bacteria? ✔Correct Answer-a bacteria that grows best with oxygen
but can grow with little to no oxygen
what is a microaerophillic bacteria? ✔Correct Answer-a bacteria that grows better with lower O2
and higher CO2
what is a capnophillic bacteria? ✔Correct Answer-A bacteria that grows best in environments with
high CO2
what is a mesophilic bacteria? ✔Correct Answer-a bacteria that grows best in moderate
temperatures 25C-40C
what is a thermophilic bacteria? ✔Correct Answer-a bacteria that grows best at high temps
typically 50C-60C
what is an autotrophic bacteria? ✔Correct Answer-a bacteria that uses light or inorganic
substances to synthesize their food or energy
, what is a heterotrophic bacteria? ✔Correct Answer-a bacteria that organic material as a source of
food and energy or more specifically carbon
what are the three types of bacterial stains? ✔Correct Answer-gram
acid fast
fluorescent
what type of stain is used most in clinical microbiology? ✔Correct Answer-the gram stain
what color is gram positive? ✔Correct Answer-purple remember positive equals purple
what color is gram negative? ✔Correct Answer-pink reddish
what kind of bacteria are acid fast stains used for? ✔Correct Answer-high lipid and wax content in
the cell wall
what is the primary stain used in acid fast? ✔Correct Answer-carbol fuchsin
what color do acid fast bacteria stain? ✔Correct Answer-red
what color do non acid fast bacteria stain? ✔Correct Answer-blue
when is acridine orange fluorescent stain used? ✔Correct Answer-when bacteria are difficult to
find using other stain techniques
when is calcofluor white fluorescent stain used? ✔Correct Answer-to identify fungal structures
what is the gram stain procedure? ✔Correct Answer-1. place smears and controls on a staining
rack over the sink.
2. flood with crystal violet for 30 seconds
3. rinse with water
4. flood with grams iodine for 30-60 secs
5. rinse with water
6. decolorize with alcohol-acetone until it runs clear then rinse with water
7. flood with safranin for 30-60 secs
8. rinse with water and dry
to remember what chemicals go first just go in alphabetical order
C(rystal violet)
G(rams iodine)
S(afranin)
what are inhibitors? ✔Correct Answer-additives that prevent the growth of a specific type of
bacteria making it easier to identify other bacterias
ex. Columbia agar containing nalidixic acid that inhibits non gram positive bacteria
