Cell Biol 311 Test 2 Assessment
Questions And Already Passed
Answers 2025-2026 Version.
Milstein's group in 1972 used a cell free mRNA synthesis that showed when RER is added to the
in vitro the protein is 20+ amino acids shorter than the mixture without RER. This was critical to
the discovery of:
a.mitochondrial targeting sequences
b. signal peptidases/signal sequences
c.SRP/SRP receptor
d. Role of GTP/GDP in the RER protein synthesis
e. Importance of trans-membrane potential across RER membrane and the role in protein
synthesis - Answer Signal peptidases/ signal sequences
Which of the following would not be a term used to describe the cell fusion experiment?
a. Antibodies
b. Freeze fracture
c. Mouse cell
d. Human cell
e. Fluorescent tags - Answer Freeze Fracture
The proper path of protein synthesis via the RER?
a. RER-TGN-Trans Golgi-Cis Golgi-secretory vesicles
b.RER-Trans-Gogli-Medial Golgi-Cis Golgi-TGN-Secretory vesicles
c.RER-Cis Golgi-Medial Golgi-Trans Golgi-Secretory vesicles
d.RER-Secretory vesicles-Cis Golgi-Medial Golgi-Trans Golgi-TGN
e.REF-TGN-Cis Golgi-Medial Golgi-Trans Golgi-Secretory vesicles - Answer RER-Cis Golgi-Medial
Golgi-Trans Golgi-TGN-secretory vesicles
In what organelle would Tim54, Tim22, Tom40, AND Tom70 be found?
a. mitochondria
b.SER
,The Semliki Forest Virus can be used as a ligand to follow which type of receptor system?
a.Transferrin
b.LDL
c.Epidermal Growth Factor
d.Insulin
e.None - Answer LDL
The experiment with 3H fucose in class showed it was added to proteins in which organelle?
a. Golgi
b. SER
c. RER
d. Storage vesicles
e. Unknown - Answer Golgi
The word connexon is used in to connection to which cell junction?
a. Gap junction
b. Hemi-desmosome
c. Neurochemical synapses
d. Desmosomes
e. Tight junctions - Answer Gap junction
Which organells below contains DNA that can transcribed/translated into proteins?
a. Lysosomes
b. Peroxides
c. Mitochondria
d. Chloroplasts
e. C and D - Answer Mitochondria and Chloroplasts
The TGN was discovered by manipulating what?
a. pH of cell culture
b. Osmolarity of cell culture
, The efficacy of tight junctions can be tested with which probes?
a. Anti-actin antibody
b. Anti-keratin antibody
c. Anti glycophorin antibody
d. Semliki forest virus
e. Sodium fluorescein - Answer Sodium fluorescein
Simon and Blobel used Ohm's law to identify the presence of what structure in the RER
membrane?
a. Translocon
b. SRP
c. SRP receptor
d. Signal peptidase
e. Nascent protein - Answer Translocon
The breakthrough identifying translocon was accomplished with what type of probe/system?
a. Antibodies
b. FRET
c. Light-activated cross-linking reagent
d. Laser capture microdissection
e. NMR microscopy - Answer Light-activated cross-linking reagent.
Mannose-6-phosphate on a protein can direct the protein to which organelle?
a. Lysosome
b. Nucleus
c. Peroxisome
d. Mitochondria
e. Chloroplast - Answer Lysosome
Time from ligand binding to hydrolysis in lysosomes for typical receptor mediated endocytosis is
?
Questions And Already Passed
Answers 2025-2026 Version.
Milstein's group in 1972 used a cell free mRNA synthesis that showed when RER is added to the
in vitro the protein is 20+ amino acids shorter than the mixture without RER. This was critical to
the discovery of:
a.mitochondrial targeting sequences
b. signal peptidases/signal sequences
c.SRP/SRP receptor
d. Role of GTP/GDP in the RER protein synthesis
e. Importance of trans-membrane potential across RER membrane and the role in protein
synthesis - Answer Signal peptidases/ signal sequences
Which of the following would not be a term used to describe the cell fusion experiment?
a. Antibodies
b. Freeze fracture
c. Mouse cell
d. Human cell
e. Fluorescent tags - Answer Freeze Fracture
The proper path of protein synthesis via the RER?
a. RER-TGN-Trans Golgi-Cis Golgi-secretory vesicles
b.RER-Trans-Gogli-Medial Golgi-Cis Golgi-TGN-Secretory vesicles
c.RER-Cis Golgi-Medial Golgi-Trans Golgi-Secretory vesicles
d.RER-Secretory vesicles-Cis Golgi-Medial Golgi-Trans Golgi-TGN
e.REF-TGN-Cis Golgi-Medial Golgi-Trans Golgi-Secretory vesicles - Answer RER-Cis Golgi-Medial
Golgi-Trans Golgi-TGN-secretory vesicles
In what organelle would Tim54, Tim22, Tom40, AND Tom70 be found?
a. mitochondria
b.SER
,The Semliki Forest Virus can be used as a ligand to follow which type of receptor system?
a.Transferrin
b.LDL
c.Epidermal Growth Factor
d.Insulin
e.None - Answer LDL
The experiment with 3H fucose in class showed it was added to proteins in which organelle?
a. Golgi
b. SER
c. RER
d. Storage vesicles
e. Unknown - Answer Golgi
The word connexon is used in to connection to which cell junction?
a. Gap junction
b. Hemi-desmosome
c. Neurochemical synapses
d. Desmosomes
e. Tight junctions - Answer Gap junction
Which organells below contains DNA that can transcribed/translated into proteins?
a. Lysosomes
b. Peroxides
c. Mitochondria
d. Chloroplasts
e. C and D - Answer Mitochondria and Chloroplasts
The TGN was discovered by manipulating what?
a. pH of cell culture
b. Osmolarity of cell culture
, The efficacy of tight junctions can be tested with which probes?
a. Anti-actin antibody
b. Anti-keratin antibody
c. Anti glycophorin antibody
d. Semliki forest virus
e. Sodium fluorescein - Answer Sodium fluorescein
Simon and Blobel used Ohm's law to identify the presence of what structure in the RER
membrane?
a. Translocon
b. SRP
c. SRP receptor
d. Signal peptidase
e. Nascent protein - Answer Translocon
The breakthrough identifying translocon was accomplished with what type of probe/system?
a. Antibodies
b. FRET
c. Light-activated cross-linking reagent
d. Laser capture microdissection
e. NMR microscopy - Answer Light-activated cross-linking reagent.
Mannose-6-phosphate on a protein can direct the protein to which organelle?
a. Lysosome
b. Nucleus
c. Peroxisome
d. Mitochondria
e. Chloroplast - Answer Lysosome
Time from ligand binding to hydrolysis in lysosomes for typical receptor mediated endocytosis is
?
