BIOC385 Exam 4 questions with
verified solutions
Which way of cleavage results in recombination of a holiday junction? - correct answer
✔✔Vertical
When you cut horizontally in a holiday junction does it result in recombination? - correct answer
✔✔No
What role do BRCA1 and BRCA2 play in DNA repair? - correct answer ✔✔assist with strand
invasion
What accounts for the high fidelity of DNA polymerization? Choose the ONE best answer -
correct answer ✔✔Active site geometry ensures that only correct sized nucleotide base pairs
are formed, and 3' to 5' proofreading removes incorrect nucleotides.
How can DNA strands be synthesized using bidirectional replication? - correct answer ✔✔They
are synthesized through Okazaki fragments.
In the figure show below of an E. coli replication fork, which protein is responsible for relieving
torsional stress? - correct answer ✔✔Gyrase
T/F This describes both DNA replication and RNA transcription in E. Coli: A specific region of the
DNA is recognized and bound by the polymerase. - correct answer ✔✔True
T/F This describes both DNA replication and RNA transcription in E. Coli: The direction of
polymerization is 5' to 3' - correct answer ✔✔True
, T/F This describes both DNA replication and RNA transcription in E. Coli: The dierection of
enzyme movement on the template strand is 3' to 5' - correct answer ✔✔True
AZT is such a good HIV drug because it - correct answer ✔✔prevents new DNA from being
replicated
The two key elements of the oriC include - correct answer ✔✔thre 13-bp repeats and four 9-bp
repeats with enriched A-T pairs
If 15N DNA replicated using conservative replication in 14N media, the outcome would be that
new DNA has - correct answer ✔✔one high density and one low density strand
T/F The function of the MutS-MutL-MutH protein complex is DNA methylation. - correct answer
✔✔T/F The function of the MutS-MutL-MutH protein complex is
T/F The function of the MutS-MutL-MutH protein complex is the resolution of Holliday
junctions. - correct answer ✔✔False
Why is the S value for the eukaryotic ribosome (80S) smaller than the sum of the S values for
the small (40S) and large (60S) ribosomal subunits? Choose the ONE best answer. - correct
answer ✔✔S values correspond to Svedberg units, which are a measure of sedimentation in a
centrifuge, however S values are not additive because shape affects sedimentation rates.
Nirenberg and colleagues determined a portion of the genetic code using filter binding assays
that included ribosomes, trinucleotides and tRNA molecules charged with radioactively-labeled
amino acids. Choose the answer below that correctly answers the following three questions in
order:
1) If the trinucleotide sequence is CAG, which amino acid will be bound to the filter paper?
2) If the wobble position of the trinucleotide sequence is changed to a U, which amino acid will
be bound to the filter paper?
verified solutions
Which way of cleavage results in recombination of a holiday junction? - correct answer
✔✔Vertical
When you cut horizontally in a holiday junction does it result in recombination? - correct answer
✔✔No
What role do BRCA1 and BRCA2 play in DNA repair? - correct answer ✔✔assist with strand
invasion
What accounts for the high fidelity of DNA polymerization? Choose the ONE best answer -
correct answer ✔✔Active site geometry ensures that only correct sized nucleotide base pairs
are formed, and 3' to 5' proofreading removes incorrect nucleotides.
How can DNA strands be synthesized using bidirectional replication? - correct answer ✔✔They
are synthesized through Okazaki fragments.
In the figure show below of an E. coli replication fork, which protein is responsible for relieving
torsional stress? - correct answer ✔✔Gyrase
T/F This describes both DNA replication and RNA transcription in E. Coli: A specific region of the
DNA is recognized and bound by the polymerase. - correct answer ✔✔True
T/F This describes both DNA replication and RNA transcription in E. Coli: The direction of
polymerization is 5' to 3' - correct answer ✔✔True
, T/F This describes both DNA replication and RNA transcription in E. Coli: The dierection of
enzyme movement on the template strand is 3' to 5' - correct answer ✔✔True
AZT is such a good HIV drug because it - correct answer ✔✔prevents new DNA from being
replicated
The two key elements of the oriC include - correct answer ✔✔thre 13-bp repeats and four 9-bp
repeats with enriched A-T pairs
If 15N DNA replicated using conservative replication in 14N media, the outcome would be that
new DNA has - correct answer ✔✔one high density and one low density strand
T/F The function of the MutS-MutL-MutH protein complex is DNA methylation. - correct answer
✔✔T/F The function of the MutS-MutL-MutH protein complex is
T/F The function of the MutS-MutL-MutH protein complex is the resolution of Holliday
junctions. - correct answer ✔✔False
Why is the S value for the eukaryotic ribosome (80S) smaller than the sum of the S values for
the small (40S) and large (60S) ribosomal subunits? Choose the ONE best answer. - correct
answer ✔✔S values correspond to Svedberg units, which are a measure of sedimentation in a
centrifuge, however S values are not additive because shape affects sedimentation rates.
Nirenberg and colleagues determined a portion of the genetic code using filter binding assays
that included ribosomes, trinucleotides and tRNA molecules charged with radioactively-labeled
amino acids. Choose the answer below that correctly answers the following three questions in
order:
1) If the trinucleotide sequence is CAG, which amino acid will be bound to the filter paper?
2) If the wobble position of the trinucleotide sequence is changed to a U, which amino acid will
be bound to the filter paper?
