MAMMALIAN GENETICS BCH5413 EXAM 2 ACTUAL 2025/2026 QUESTIONS AND 100% CORRECT ANSWERS

MAMMALIAN GENETICS BCH5413 EXAM 2 ACTUAL 2025/2026 QUESTIONS AND 100% CORRECT ANSWERS What is the meaning of the term transgenic? - Answer -Foreign DNA is inserted into the genome Once an oocyte has been injected with a transgene and implanted into a mouse, what are the steps to obtaining offspring? - Answer -The transgenic mouse is screened to confirm the transgenic gene is present, then it is crossed with another transgenic mouse to start a line of transgenic mice T/F: the transgene is randomly integrated into a mouse genome. - Answer -True (this integration is random)Need assistance on Online classes, Exams & Assignments? Reach out for instant help!! Full Course Assistance, Plagiarism-free Essay Writing, Research Paper, Dissertation, Discussion Posts, etc…. Confidential & Secure services. Tutors are available for all subjects! Email now at: The goal of creating a transgenic mouse is to study ___ of function. - Answer -Gain The goal of creating a knockout mouse is to study ___ of function. - Answer -Loss T/F: the mouse that a transgene is being added to already has 2 copies of that gene. - Answer -True (the transgene will cause overexpression by increasing copy number of the gene) Once an oocyte has been injected with a transgene and implanted into a mouse, what are the 2 possible ways to determine which offspring have the transgene? - Answer -(1) PCR: SP6 and T7 primers will only amplify the transgene (bac vector) and beta-globin primers will only amplify the endogenous gene; (2) Southern blot: probes specific to either the endogenous gene or transgene are used T/F: in a gel of PCR of a transgenic mouse that HASN'T been injected with the bac vector, there should be bands for both the SP6 and T7 primers. - Answer -False (SP6 and T7 sites are only found in the bac vector, not the endogenous gene)T/F: in a gel of PCR that HASN'T been injected with the bac vector, there should only be a band for beta-globin. - Answer -True (beta-globin is only present in the genome, not in the bac vector) How is the transgene incorporated into a mouse genome? - Answer - The gene of interest is cloned into a bac vector, microinjected into a mouse embryo, and transplanted into a female mouse who will carry the embryo to term In transgenic mice, how could yo