How ? Paper Chromatography two-Way Chromatography
* mixture is added toamobile phase * stationary phase :
chromatography paper * When only one solvent used , may not
f. g. liquid or gas * mobile phase : suitable solvent / water ethanol , ,
etc ) be separated sufficiently
"
A separation : as solvent moves up paper it dissolves * chromatogram 11 solvent) rotated 90°
By adsorption : the components + moves up paper .
+ is further developed w/ 2nd solvent
* components bind to surface of solid sp .
* more soluble component further it ,
moves #
* stronger the adsorption to S.p. more * Diagram snows that after further
,
the component molecules are slowed Rg Values → location of compound on Chromatogram development → substance cismixotc.tk
By relative solubility : distance travelled by the component d * Used to separate mixtures in which Lor more
pg , =
* components dissolve in liquids .p .
distance travelled by the solvent do compounds have similar Rs values
* greater the solubility insp .
-_ Slower
Thin Layer Chromatography
* monitor extent otchenicalrxn
check purity of compound
or to
Chromatography Retention
A time
4> Measured from
.
time
taken from injection until compound detected
time sample injected to time
* preparative TLC purify gramamounhoi organic
its peak shows mat height
has liquid Chromatography
-
compounds
* Stationary phase : liquid adsorbed Onan inert solid support
Depends on :
Stationaru silica / sioiloraluminaltthogl A mobile phase gas : A boiling point :
high b.pt =
long RT
Phase mounted on glass plate / plastic strip
A solubility in liquid phase :
greater solubility =
long RT
Mobile Overview :
suitable organic solvent
phase * small amount of sample injected into Glcmacnine Interpretation 0161C Chromatogram
separation similar to paper but of
,
a B injector contained in an oven * Each compound in Mit will produce apeak
higher duality * sample vapourised Areas under peaks proportional to amount Normand
+ carried along thin column by inert carrier gas *
* column contains a liquid S-P.iadsorbedomoinerrsnidlsih.cat A RTs used to identify compounds found
-
out by pawing
limitation, similar to paper
* time taken to travel through tube depends on how much time known compounds through system under similar conditions
spent moving wlgaslm.pl rather than in liquid Is .p ) .
Amount Otcompound area under compound 's peak
:
10°
* Used to separate mixtures or volatile liquids and/or gases in sample total area underneath
* won't separate non volatile compounds e. g. carboxylic acids
- +
amino acids / Martini tutors.p ) .
Nhn
* mixture is added toamobile phase * stationary phase :
chromatography paper * When only one solvent used , may not
f. g. liquid or gas * mobile phase : suitable solvent / water ethanol , ,
etc ) be separated sufficiently
"
A separation : as solvent moves up paper it dissolves * chromatogram 11 solvent) rotated 90°
By adsorption : the components + moves up paper .
+ is further developed w/ 2nd solvent
* components bind to surface of solid sp .
* more soluble component further it ,
moves #
* stronger the adsorption to S.p. more * Diagram snows that after further
,
the component molecules are slowed Rg Values → location of compound on Chromatogram development → substance cismixotc.tk
By relative solubility : distance travelled by the component d * Used to separate mixtures in which Lor more
pg , =
* components dissolve in liquids .p .
distance travelled by the solvent do compounds have similar Rs values
* greater the solubility insp .
-_ Slower
Thin Layer Chromatography
* monitor extent otchenicalrxn
check purity of compound
or to
Chromatography Retention
A time
4> Measured from
.
time
taken from injection until compound detected
time sample injected to time
* preparative TLC purify gramamounhoi organic
its peak shows mat height
has liquid Chromatography
-
compounds
* Stationary phase : liquid adsorbed Onan inert solid support
Depends on :
Stationaru silica / sioiloraluminaltthogl A mobile phase gas : A boiling point :
high b.pt =
long RT
Phase mounted on glass plate / plastic strip
A solubility in liquid phase :
greater solubility =
long RT
Mobile Overview :
suitable organic solvent
phase * small amount of sample injected into Glcmacnine Interpretation 0161C Chromatogram
separation similar to paper but of
,
a B injector contained in an oven * Each compound in Mit will produce apeak
higher duality * sample vapourised Areas under peaks proportional to amount Normand
+ carried along thin column by inert carrier gas *
* column contains a liquid S-P.iadsorbedomoinerrsnidlsih.cat A RTs used to identify compounds found
-
out by pawing
limitation, similar to paper
* time taken to travel through tube depends on how much time known compounds through system under similar conditions
spent moving wlgaslm.pl rather than in liquid Is .p ) .
Amount Otcompound area under compound 's peak
:
10°
* Used to separate mixtures or volatile liquids and/or gases in sample total area underneath
* won't separate non volatile compounds e. g. carboxylic acids
- +
amino acids / Martini tutors.p ) .
Nhn
