EIEI.it
IlEiiitTlIlE EIil
I ftp.illfiEIileIIIIIlEI
RECOMBINANTDNATECHNOLOGY
Mankindalteringgenomesbybiotechnological processes
transformationdone with recombinantDNAtechnology
used to introduce new beneficialgenesinto an organism to create
IT igYesimnIfiegnGr8aih'she into the cells of another
organismvia a vector
Hemophilia instructions of inserted gene makes copies ofprotein for
whichgenecodes
EXAMLES Proteins made
Used in treatmentof
I fin
FactorVill
Pitti YsStirethchoersmultiple scierosis
Hemophilia B
ManufacturingHumanInsulin
I DNACodingforinsulin production is extracted from healthyhuman
pancreatic cells
2 Restrictionenzymes cut the DNA to isolate thespecificinsulingene
3 EColiplasmid isremoved cut open with enzymes
4 Theenzymeligase is used tojoin the insulin gene to the plasmid
5 The plas vector is now reinserted into the Ecoliwhichwill then
program the bacteria to produceinsulin
, TheGMbacteria are kept in large tanks in optimalconditions
allows for rapid reproduction insulin production
ADVANTAGES
producedrapidlyinlargequantities
relativelyinexpensive
fewsideeffects humaninsulin
GENETHERAPY
Techniquereplaces a faulty gene addsnew one
curedisease
improvebodiesability tofightdisease
CELLBASEDDELIVERY DIRECTDELIVERY
Sampleofpatientscells Vector isinjected given
removed exposed to intravenouslydirectly
the vector Thosecells intospecific tissue
returned to patient where taken up
GENETICALLYMODIFIEDPLANTS
means of artificialselection farmerscontrolledreproduction of
plants to ensure manyoffspring has favourablegenes
selective breeding
GMOCROPS
Tradmethodsslow less predictable
conventional breeding closelyrelated plants
geneticengineeringgenesfromany organism can be used in plant
Resultingtransformed plant is a geneticallymodifiedorganism
insertedgeneis called transgene
Transgenictechnology is unconventional because geneticmaterial
across organisms are used
GeneGunMethod Recombinant DNAtechniques
Pellets of metal coated Morecontrolledway ofgenetic
with desirable DNA are manipulationusing enzymes
f
IlEiiitTlIlE EIil
I ftp.illfiEIileIIIIIlEI
RECOMBINANTDNATECHNOLOGY
Mankindalteringgenomesbybiotechnological processes
transformationdone with recombinantDNAtechnology
used to introduce new beneficialgenesinto an organism to create
IT igYesimnIfiegnGr8aih'she into the cells of another
organismvia a vector
Hemophilia instructions of inserted gene makes copies ofprotein for
whichgenecodes
EXAMLES Proteins made
Used in treatmentof
I fin
FactorVill
Pitti YsStirethchoersmultiple scierosis
Hemophilia B
ManufacturingHumanInsulin
I DNACodingforinsulin production is extracted from healthyhuman
pancreatic cells
2 Restrictionenzymes cut the DNA to isolate thespecificinsulingene
3 EColiplasmid isremoved cut open with enzymes
4 Theenzymeligase is used tojoin the insulin gene to the plasmid
5 The plas vector is now reinserted into the Ecoliwhichwill then
program the bacteria to produceinsulin
, TheGMbacteria are kept in large tanks in optimalconditions
allows for rapid reproduction insulin production
ADVANTAGES
producedrapidlyinlargequantities
relativelyinexpensive
fewsideeffects humaninsulin
GENETHERAPY
Techniquereplaces a faulty gene addsnew one
curedisease
improvebodiesability tofightdisease
CELLBASEDDELIVERY DIRECTDELIVERY
Sampleofpatientscells Vector isinjected given
removed exposed to intravenouslydirectly
the vector Thosecells intospecific tissue
returned to patient where taken up
GENETICALLYMODIFIEDPLANTS
means of artificialselection farmerscontrolledreproduction of
plants to ensure manyoffspring has favourablegenes
selective breeding
GMOCROPS
Tradmethodsslow less predictable
conventional breeding closelyrelated plants
geneticengineeringgenesfromany organism can be used in plant
Resultingtransformed plant is a geneticallymodifiedorganism
insertedgeneis called transgene
Transgenictechnology is unconventional because geneticmaterial
across organisms are used
GeneGunMethod Recombinant DNAtechniques
Pellets of metal coated Morecontrolledway ofgenetic
with desirable DNA are manipulationusing enzymes
f
