CHE 4342 EXAM 2 QUESTIONS AND 100%
CORRECT ANSWERS!!
What makes Dicer an effective enzyme?
A. Its ability to bind all ssRNA strands
B. The absence of a nucleotide overhang
C. Its binding following the incorporation of the RISC complex
D. Correspondence between the PAZ and RNase III distance with length of siRNA
fragment
E. Its activation of Slicer
D. Correspondence between the PAZ and RNase III distance with length of siRNA fragment
An interferon response, a form of innate immunity that leads to nonspecific reactions with
foreign genetic material, was seen in mammalian cells. What was the cause of this
response?
A. Off-target effects of CRISPR
B. Gene knockout by RNAi
C. tracrRNA binding onto genomic target site
D. A mutation in the H1 histone linker protein
E. Mutation in a chromatin remodeling complex
A. Off-target effects of CRISPR
There was a mutation found on EF-TU. What will be an effect seen in the cell?
A. Inability for the 50S subunit to bind to the 30S subunit
B. fMet-tRNA will not be incorporated into the ribosome
C. Incomplete binding of aa-tRNA to ribosome
D. Increased GTP hydrolysis
E. Heightened rate of translocation seen in the ribosome
C. Incomplete binding of aa-tRNA to ribosome
Which of the following molecules is NOT involved in Pentose Phosphate Pathway?
,A) Glucose-6-phosphate
B) Fructose-6-phosphate
C) Glyceraldehyde-3-phosphate
D) 3-Phosphoglycerate
E) 6-Phosphogluconate
D) 3-Phosphoglycerate
Which of the following statements about lipid metabolism is FALSE?
A) Franz Knoop experiment used chemical labels to trace metabolic pathways.
B) Fatty acids are progressively broken down in a 2-carbon mechanism.
C) Fatty acids are activated by acyl-CoA synthetase.
D) The final product of fatty acid oxidation is acetyl-CoA.
E) Ketone bodies are produced in low concentrations of acetyl-CoA.
E) Ketone bodies are produced in low concentrations of acetyl-CoA.
Which of the following atoms in purine is derived from formate?
A) C5
B) C6
C) C8
D) N1
E) N9
C) C8
Which of the following orders of DNA packaging is a correct?
A) Nucleosomes → chromosome → 30nm fiber → loops → continued looping
B) Nucleosomes → loops → continued looping → 30nm fiber → chromosome
C) Nucleosomes → 30nm fiber → loops → continued looping → chromosome
D) 30nm fiber → nucleosomes → loops → continued looping → chromosome
E) 30nm fiber → loops → continued looping → nucleosomes → chromosome
, C) Nucleosomes → 30nm fiber → loops → continued looping → chromosome
Which of the following pairs of DNA damage and its repair pathway is incorrectly
matched?
A) Alkylating agents: Base-excision repair
B) Replication errors: Mismatch repair
C) Bulky adduct: Nucleotide-excision repair
D) Single-strand break: Recombinational repair
E) 8-Oxoguanine: Base-excision repair
D) Single-strand break: Recombinational repair
The RISC effector complex can cause
I. Post-transcriptional cleavage of mRNA
II. Translational repression of the mRNA
III. Transcriptional silencing
A) Only I
B) Only II
C) I, II
D) II, III
E) I, II, III
E) I, II, III
Which of the following usages of siRNA is INCORRECT?
A) Gene knockdowns
B) Gene knockins
C) Off-target effects
D) Therapeutic techniques
E) Analysis of signal transduction pathways
B) Gene knockins
CORRECT ANSWERS!!
What makes Dicer an effective enzyme?
A. Its ability to bind all ssRNA strands
B. The absence of a nucleotide overhang
C. Its binding following the incorporation of the RISC complex
D. Correspondence between the PAZ and RNase III distance with length of siRNA
fragment
E. Its activation of Slicer
D. Correspondence between the PAZ and RNase III distance with length of siRNA fragment
An interferon response, a form of innate immunity that leads to nonspecific reactions with
foreign genetic material, was seen in mammalian cells. What was the cause of this
response?
A. Off-target effects of CRISPR
B. Gene knockout by RNAi
C. tracrRNA binding onto genomic target site
D. A mutation in the H1 histone linker protein
E. Mutation in a chromatin remodeling complex
A. Off-target effects of CRISPR
There was a mutation found on EF-TU. What will be an effect seen in the cell?
A. Inability for the 50S subunit to bind to the 30S subunit
B. fMet-tRNA will not be incorporated into the ribosome
C. Incomplete binding of aa-tRNA to ribosome
D. Increased GTP hydrolysis
E. Heightened rate of translocation seen in the ribosome
C. Incomplete binding of aa-tRNA to ribosome
Which of the following molecules is NOT involved in Pentose Phosphate Pathway?
,A) Glucose-6-phosphate
B) Fructose-6-phosphate
C) Glyceraldehyde-3-phosphate
D) 3-Phosphoglycerate
E) 6-Phosphogluconate
D) 3-Phosphoglycerate
Which of the following statements about lipid metabolism is FALSE?
A) Franz Knoop experiment used chemical labels to trace metabolic pathways.
B) Fatty acids are progressively broken down in a 2-carbon mechanism.
C) Fatty acids are activated by acyl-CoA synthetase.
D) The final product of fatty acid oxidation is acetyl-CoA.
E) Ketone bodies are produced in low concentrations of acetyl-CoA.
E) Ketone bodies are produced in low concentrations of acetyl-CoA.
Which of the following atoms in purine is derived from formate?
A) C5
B) C6
C) C8
D) N1
E) N9
C) C8
Which of the following orders of DNA packaging is a correct?
A) Nucleosomes → chromosome → 30nm fiber → loops → continued looping
B) Nucleosomes → loops → continued looping → 30nm fiber → chromosome
C) Nucleosomes → 30nm fiber → loops → continued looping → chromosome
D) 30nm fiber → nucleosomes → loops → continued looping → chromosome
E) 30nm fiber → loops → continued looping → nucleosomes → chromosome
, C) Nucleosomes → 30nm fiber → loops → continued looping → chromosome
Which of the following pairs of DNA damage and its repair pathway is incorrectly
matched?
A) Alkylating agents: Base-excision repair
B) Replication errors: Mismatch repair
C) Bulky adduct: Nucleotide-excision repair
D) Single-strand break: Recombinational repair
E) 8-Oxoguanine: Base-excision repair
D) Single-strand break: Recombinational repair
The RISC effector complex can cause
I. Post-transcriptional cleavage of mRNA
II. Translational repression of the mRNA
III. Transcriptional silencing
A) Only I
B) Only II
C) I, II
D) II, III
E) I, II, III
E) I, II, III
Which of the following usages of siRNA is INCORRECT?
A) Gene knockdowns
B) Gene knockins
C) Off-target effects
D) Therapeutic techniques
E) Analysis of signal transduction pathways
B) Gene knockins
