CHE 4342 EXAM REVISION QUESTIONS
AND 100% CORRECT ANSWERS!!
1. Which of the following is a FALSE statement regarding glycogen breakdown?
a. Glycogen breakdown cleaves 𝛼(1→6) linkages to form 𝛼 (1→4) linkages
b. Glycogen breakdown involves the formation of a Schiff base
c. Glycogen breakdown uses UTP in place of ATP
d. Phosphoglucomutase converts G1P to G6P
c. Glycogen breakdown uses UTP in place of ATP
2. 𝛃-oxidation of saturated fatty acids differs from that of unsaturated fatty acids in that...
a. Unsaturated fatty acid oxidation produces FADH2 while saturated fatty acid oxidation
produces only NADH
b. Unsaturated fatty acid oxidation utilizes enoyl-CoA isomerase to convert a cis-bond to a
trans-bond, while this isomerase is never used in saturated fatty acid synthesis
c. SyccinylCoA is used by only saturated fatty acid oxidation and is not seen in unsaturated
fatty acid oxidation
d. Saturated fatty acids are more easily oxidized than unsaturated fatty acids as
unsaturated fatty acids must be isomerized to prevent reverse reactions
d. Saturated fatty acids are more easily oxidized than unsaturated fatty acids as unsaturated fatty
acids must be isomerized to prevent reverse reactions
3. Which of the following are spontaneous forms of DNA damage?
a. Base tautomerization
b. UV radiation
c. Base deamination
d. None of the above
e. A and C
e. A and C
4. Which of the following forms of DNA damage result in DNA base adducts?
a. Ultra-violet Light
,b. Ionising Radiation
c. Alkaylating agents
d. None of the above
Ultra-violet Light
5. Ubiquitination serves an important role in protein degradation. Which of the following
statements of ubiquitination is true?
a. Ubiquitin binds to a serine residue on the protein designated to be degraded
b. Ubiquitin is large, making it favorable for destruction in the cell
c. Only polyubiquitin is possible in mammalian cells
d. Ubiquination is activated by adenylate
d. Ubiquination is activated by adenylate
6. Which of the following statements of RNAi is FALSE?
a. miRNA is triggered by dsRNA, while siRNA is triggered by ssRNA
b. miRNA is preceded by pri-miRNA that is then cleaved by Drosha, while siRNA is
preceded by dsRNA that is cleaved by Dicer
c. siRNA is exogenous while miRNA is endogenous
d. All of the above are false statements
a. miRNA is triggered by dsRNA, while siRNA is triggered by ssRNA
7. Which of the following are potential antibiotic targets in bacterial cells?
a. The cell wall
b. The A site of the ribosome
c. The P site of the ribosome
d. The exit tunnel fo the ribosome
e. All of the above
e. All of the above
8. Which of the following statements most accurately describes wobble theory?
a. Any deviation in any position results in "wobbly", weaker base pairing that can be
broken more easily
, b. The first two amino acid positions typically exhibit wobble, while the third position must
bind in Watson/Crick base pairing
c. The third position typically exhibits wobble, while the first two positions adhere to W/C
base pairing
d. Any deviation in the third position prevents the tRNA from reading the codons, resulting
in "wobble" in translation as the ribosome is forced to slow protein synthesis
c. The third position typically exhibits wobble, while the first two positions adhere to W/C base
pairing
9. Which of the following statements about translation is/are true?
i. ATP hydrolysis drives the addition of charged tRNA molecules to the A-site.
ii. Both the A and P site interacts with mRNA.
iii. The growing polypeptide chain is released from the E site of the ribosome.
a. I, II, III
b. I, III
c. II, III
d. I only
e. II only
e. II only
Which of the following is an intermediate in the conversion of ribose-5-phosphate to GTP
following protein synthesis?
a. XMP
b. GTP
c. IMP
d. All of the above are true
e. None of the above are true
d. All of the above are true
1. Cloning vectors must have
a. Origin of replication
b. Selectable marker
AND 100% CORRECT ANSWERS!!
1. Which of the following is a FALSE statement regarding glycogen breakdown?
a. Glycogen breakdown cleaves 𝛼(1→6) linkages to form 𝛼 (1→4) linkages
b. Glycogen breakdown involves the formation of a Schiff base
c. Glycogen breakdown uses UTP in place of ATP
d. Phosphoglucomutase converts G1P to G6P
c. Glycogen breakdown uses UTP in place of ATP
2. 𝛃-oxidation of saturated fatty acids differs from that of unsaturated fatty acids in that...
a. Unsaturated fatty acid oxidation produces FADH2 while saturated fatty acid oxidation
produces only NADH
b. Unsaturated fatty acid oxidation utilizes enoyl-CoA isomerase to convert a cis-bond to a
trans-bond, while this isomerase is never used in saturated fatty acid synthesis
c. SyccinylCoA is used by only saturated fatty acid oxidation and is not seen in unsaturated
fatty acid oxidation
d. Saturated fatty acids are more easily oxidized than unsaturated fatty acids as
unsaturated fatty acids must be isomerized to prevent reverse reactions
d. Saturated fatty acids are more easily oxidized than unsaturated fatty acids as unsaturated fatty
acids must be isomerized to prevent reverse reactions
3. Which of the following are spontaneous forms of DNA damage?
a. Base tautomerization
b. UV radiation
c. Base deamination
d. None of the above
e. A and C
e. A and C
4. Which of the following forms of DNA damage result in DNA base adducts?
a. Ultra-violet Light
,b. Ionising Radiation
c. Alkaylating agents
d. None of the above
Ultra-violet Light
5. Ubiquitination serves an important role in protein degradation. Which of the following
statements of ubiquitination is true?
a. Ubiquitin binds to a serine residue on the protein designated to be degraded
b. Ubiquitin is large, making it favorable for destruction in the cell
c. Only polyubiquitin is possible in mammalian cells
d. Ubiquination is activated by adenylate
d. Ubiquination is activated by adenylate
6. Which of the following statements of RNAi is FALSE?
a. miRNA is triggered by dsRNA, while siRNA is triggered by ssRNA
b. miRNA is preceded by pri-miRNA that is then cleaved by Drosha, while siRNA is
preceded by dsRNA that is cleaved by Dicer
c. siRNA is exogenous while miRNA is endogenous
d. All of the above are false statements
a. miRNA is triggered by dsRNA, while siRNA is triggered by ssRNA
7. Which of the following are potential antibiotic targets in bacterial cells?
a. The cell wall
b. The A site of the ribosome
c. The P site of the ribosome
d. The exit tunnel fo the ribosome
e. All of the above
e. All of the above
8. Which of the following statements most accurately describes wobble theory?
a. Any deviation in any position results in "wobbly", weaker base pairing that can be
broken more easily
, b. The first two amino acid positions typically exhibit wobble, while the third position must
bind in Watson/Crick base pairing
c. The third position typically exhibits wobble, while the first two positions adhere to W/C
base pairing
d. Any deviation in the third position prevents the tRNA from reading the codons, resulting
in "wobble" in translation as the ribosome is forced to slow protein synthesis
c. The third position typically exhibits wobble, while the first two positions adhere to W/C base
pairing
9. Which of the following statements about translation is/are true?
i. ATP hydrolysis drives the addition of charged tRNA molecules to the A-site.
ii. Both the A and P site interacts with mRNA.
iii. The growing polypeptide chain is released from the E site of the ribosome.
a. I, II, III
b. I, III
c. II, III
d. I only
e. II only
e. II only
Which of the following is an intermediate in the conversion of ribose-5-phosphate to GTP
following protein synthesis?
a. XMP
b. GTP
c. IMP
d. All of the above are true
e. None of the above are true
d. All of the above are true
1. Cloning vectors must have
a. Origin of replication
b. Selectable marker
