BIOL230 LAB FINAL QUESTIONS AND VERIFIED SOLUTIONS 2
"You pass through a hole, the size of which can be manipulated with a sliding flap, it is the
_______" - ✔✔Diaphragm "You get bent through a piece of glass known as the_______" -
✔✔Condenser "You pass through a thin pane of glass with a bunch of single-celled life
forms, must've been the ________" - ✔✔Slide "Luckily, you're received by a metal tube on a
spinning assembly of others like it. They're the _______" - ✔✔Objectives "And finally you're
split through two identical lenses known as the _______" - ✔✔Oculars Give two ways to
adjust the lighting on the scope. - ✔✔Iris diaphragm Light intensity obj. Define or explain
the goal of aseptic technique. - ✔✔To minimize risk of contamination. Alby uncaps her
tubes in preparation for the cell transfer while her loop cooks. Explain why this is good
and/or bad. - ✔✔Unnecessary exposure time. Jarnes loop sizzles when it makes contact
with his bacterial culture. Give two reasons why this is bad. - ✔✔1. Destroying poor cells ->
garbage might show up in stained samples. 2. Aerosolizing liquid culture for self to inhale &
spread. When looking at a slide under the scope, you should start with the stage all the way
up because .... - ✔✔Then you have only 1 way to go while watching image carefully. You
are looking for the right stage height so that your slide is in focus. You should NOT look
away from the oculars while turning the adjustment knob because .... - ✔✔So you don't
miss that moment of clarity. lol 38.9 millimeters = ??? kilometers - ✔✔0.0000389
kilometers 6.44 kilometers = ??? centimeters - ✔✔644000 centimeters 929 microliters
= ??? deciliters - ✔✔0.00929 deciliters 8640 milligrams = ??? decigrams - ✔✔86.40
decigrams How might adopting the metric system help humanity (as a whole) become
more comfortable with quantitative science? - ✔✔- More intuitive understanding of scale. -
Ease of calculation/conversion What is the difference between putting your slides on the
warmer and passing them over a flame? - ✔✔Warmer: dries off fluid Flame: fixes & kills
Define/explain autolysis by breaking the word down. - ✔✔Auto: self Lysis: break down You
find an old bottle of stain called St. Louise Blues (SLB). The label says: Na*+ Some Blue
Stuff (SBS)*- What kind of simple staining would SLB be used for and why? - ✔✔SBS is (-)
negative, so SLB will be for indirect staining. SBS: chromophore You messed up and used
nigrosine instead of crystal violet while following a direct staining procedure. What do you
expect to see on those slides and why? Will there be cells on the finished slide? - ✔✔I
expect to see unstained cells? or nothing because the nigrosine would have been rinsed
off. Yes, there will be cells on the finished slide because I fixed it. Why is iodine important
in gram staining? - ✔✔Mordant, binds to CV so gram + retain color. After gram staining,
what colors are gram + and gram - cells supposed to be? - ✔✔Gram + = purple Gram - =
pink You are given a single tube of culture for a direct staining assignment with methylene
"You pass through a hole, the size of which can be manipulated with a sliding flap, it is the
_______" - ✔✔Diaphragm "You get bent through a piece of glass known as the_______" -
✔✔Condenser "You pass through a thin pane of glass with a bunch of single-celled life
forms, must've been the ________" - ✔✔Slide "Luckily, you're received by a metal tube on a
spinning assembly of others like it. They're the _______" - ✔✔Objectives "And finally you're
split through two identical lenses known as the _______" - ✔✔Oculars Give two ways to
adjust the lighting on the scope. - ✔✔Iris diaphragm Light intensity obj. Define or explain
the goal of aseptic technique. - ✔✔To minimize risk of contamination. Alby uncaps her
tubes in preparation for the cell transfer while her loop cooks. Explain why this is good
and/or bad. - ✔✔Unnecessary exposure time. Jarnes loop sizzles when it makes contact
with his bacterial culture. Give two reasons why this is bad. - ✔✔1. Destroying poor cells ->
garbage might show up in stained samples. 2. Aerosolizing liquid culture for self to inhale &
spread. When looking at a slide under the scope, you should start with the stage all the way
up because .... - ✔✔Then you have only 1 way to go while watching image carefully. You
are looking for the right stage height so that your slide is in focus. You should NOT look
away from the oculars while turning the adjustment knob because .... - ✔✔So you don't
miss that moment of clarity. lol 38.9 millimeters = ??? kilometers - ✔✔0.0000389
kilometers 6.44 kilometers = ??? centimeters - ✔✔644000 centimeters 929 microliters
= ??? deciliters - ✔✔0.00929 deciliters 8640 milligrams = ??? decigrams - ✔✔86.40
decigrams How might adopting the metric system help humanity (as a whole) become
more comfortable with quantitative science? - ✔✔- More intuitive understanding of scale. -
Ease of calculation/conversion What is the difference between putting your slides on the
warmer and passing them over a flame? - ✔✔Warmer: dries off fluid Flame: fixes & kills
Define/explain autolysis by breaking the word down. - ✔✔Auto: self Lysis: break down You
find an old bottle of stain called St. Louise Blues (SLB). The label says: Na*+ Some Blue
Stuff (SBS)*- What kind of simple staining would SLB be used for and why? - ✔✔SBS is (-)
negative, so SLB will be for indirect staining. SBS: chromophore You messed up and used
nigrosine instead of crystal violet while following a direct staining procedure. What do you
expect to see on those slides and why? Will there be cells on the finished slide? - ✔✔I
expect to see unstained cells? or nothing because the nigrosine would have been rinsed
off. Yes, there will be cells on the finished slide because I fixed it. Why is iodine important
in gram staining? - ✔✔Mordant, binds to CV so gram + retain color. After gram staining,
what colors are gram + and gram - cells supposed to be? - ✔✔Gram + = purple Gram - =
pink You are given a single tube of culture for a direct staining assignment with methylene
