Applied Science BTEC - Unit 6 - Learning Aim B - Produce a plan for an investigative project based on the proposal - DISTINCTION

, Name:

People in your group:

Practical name: Amylase at different temperatures

Hypothesis: The amylase activity will increase while the temperature increases

Null hypothesis (if you are going to do a There is no significant difference in the activity of amylase at different temperatures.
statistical analysis):

Dependant variable: 1. Independent Variable: Temperature (the different temperatures at which you will test the amylase activity).

Independent Variable: . Dependent Variable: Amylase activity (this can be measured by the rate of starch breakdown, often indicated by the time
taken for a colour change when iodine is added).


Control Variable: Controlled Variables: These are factors that should be kept constant throughout the experiment, such as:
- Concentration of the amylase solution
- Concentration of the starch solution
- Volume of solutions used
- Duration of the reaction
- pH level of the solution

Equipment: . Water bath or heating device
2. Thermometer
3. Test tubes or reaction vessels
4. Pipettes or syringes
5. Iodine solution
6. Stopwatch or timer
7. Stirring rod or magnetic stirrer
8. pH meter or pH strips
Chemicals: 1.. Amylase solution
2. Starch solution
3. Iodine solution