Holechek Bio 340 Exam 3
Study online at https://quizlet.com/_7ffan1
This technique uses an extremophile in
PCR
order to replicate DNA quickly.
The first thing that happens to DNA dur-
denaturation
ing the PCR process is ____?
The second thing that happens during
primer annealing
the PCR process is ____?
phase in PCR during which a primer
primer annealing
binds to a template strand
The final step of the PCR process is
extension
termed ____?
what type of electric charge does DNA
negative
have?
Other than charge what is a crucial com-
size ponent of DNA during gel electrophore-
sis?
Short DNA fragments that can be stained
with something such as a radioactive dye
Probes
in order to further understand DNA are
called
Transfer of replicated DNA from a donor
Conjugation
to a recipient
In bacteria, the direct transfer of DNA
Conjugation between two cells that are temporarily
joined.
What important structure is used to
Pilus transfer replicated DNA during conjuga-
tion
Where does the DNA come from during
enviroment
transformation?
Transformation can be done with DNA
Plasmid
fragments OR a DNA ____
DNA is transferred from a donor cell to a
Transduction
recipient via a bacteriophage
1/7
, Holechek Bio 340 Exam 3
Study online at https://quizlet.com/_7ffan1
Transfer of DNA from one bacterium to
Transduction
another using a viral vector is called ____
Sometimes plasmids can cause prob-
lems when they contain harmful informa-
antibiotic resistance tion because when treating the patient its
found these plasmids have ____ ____(
two words)
region in plasmid that has been engi-
multiple cloning site neered to contain recognition sites of a
number of restriction endonucleases
The most common reporter gene used in
Lac Z
plasmids is the ___ _ operon
During white/blue screening which of the
white
two contains the recombinant plasmids?
Bacteriophages look like a creepy crea-
The matrix ture out of what film series featuring
keanu reeves?
reading DNA ends up being the same
palindromic backwards and forwards earns the DNA
the descriptive term ____
The enzyme that is crucial for piecing
Ligase
DNA back together is called ____
Which is more effecient in terms of being
sticky pieced back together, blunt ends or sticky
ends?(one word answer)
Maybe there is a gene you want to ex-
press or simply isolate for a time. What is
Plasmid
the name of the thing you essentially put
this DNA in for a short time?
Which activity of DNA polymerase is re-
3' to 5' exonuclease sponsible for its ability to proofread newly
synthesized DNA?
600000
2/7
Study online at https://quizlet.com/_7ffan1
This technique uses an extremophile in
PCR
order to replicate DNA quickly.
The first thing that happens to DNA dur-
denaturation
ing the PCR process is ____?
The second thing that happens during
primer annealing
the PCR process is ____?
phase in PCR during which a primer
primer annealing
binds to a template strand
The final step of the PCR process is
extension
termed ____?
what type of electric charge does DNA
negative
have?
Other than charge what is a crucial com-
size ponent of DNA during gel electrophore-
sis?
Short DNA fragments that can be stained
with something such as a radioactive dye
Probes
in order to further understand DNA are
called
Transfer of replicated DNA from a donor
Conjugation
to a recipient
In bacteria, the direct transfer of DNA
Conjugation between two cells that are temporarily
joined.
What important structure is used to
Pilus transfer replicated DNA during conjuga-
tion
Where does the DNA come from during
enviroment
transformation?
Transformation can be done with DNA
Plasmid
fragments OR a DNA ____
DNA is transferred from a donor cell to a
Transduction
recipient via a bacteriophage
1/7
, Holechek Bio 340 Exam 3
Study online at https://quizlet.com/_7ffan1
Transfer of DNA from one bacterium to
Transduction
another using a viral vector is called ____
Sometimes plasmids can cause prob-
lems when they contain harmful informa-
antibiotic resistance tion because when treating the patient its
found these plasmids have ____ ____(
two words)
region in plasmid that has been engi-
multiple cloning site neered to contain recognition sites of a
number of restriction endonucleases
The most common reporter gene used in
Lac Z
plasmids is the ___ _ operon
During white/blue screening which of the
white
two contains the recombinant plasmids?
Bacteriophages look like a creepy crea-
The matrix ture out of what film series featuring
keanu reeves?
reading DNA ends up being the same
palindromic backwards and forwards earns the DNA
the descriptive term ____
The enzyme that is crucial for piecing
Ligase
DNA back together is called ____
Which is more effecient in terms of being
sticky pieced back together, blunt ends or sticky
ends?(one word answer)
Maybe there is a gene you want to ex-
press or simply isolate for a time. What is
Plasmid
the name of the thing you essentially put
this DNA in for a short time?
Which activity of DNA polymerase is re-
3' to 5' exonuclease sponsible for its ability to proofread newly
synthesized DNA?
600000
2/7
