Cell bio 353 Exam 1 UPDATED ACTUAL
Exam Questions and CORRECT Answers
phase contrast microscope: state of cell, resolution, steps for preparation/fixation - CORRECT
ANSWER -Live, 0.2 nm, no fixation, live cell imaging, limited resoluton
Amplitude Contrast Microscope: state of cell, resolution, steps for preparation/fixation -
CORRECT ANSWER -Dead, 0.2 nm, Fix-dehydrate-embed in parafinn-section-stain, cells
are preserved and can be stored for reexamination, cell/tissues r dead
Fluorescence/Confocal Microscopy: state of cell, resolution, steps for preparation/fixation -
CORRECT ANSWER -Live (GFP) dead (immuno), 0.2nm, transform cells with florescent
protein gene OR fix cells for indirect immuno stain, image specific macromolecules and
orgenelles, cost
Transmission Electron Microscope (TEM): state of cell, resolution, steps for preparation/fixation
- CORRECT ANSWER -dead, 0.2nm, Fix-dehydrate-embed in parafinn-section-stain,
high resolution of internal cellular structure, cost and cells are dead and challenging
Scanning Electron Microscope (SEM): state of cell, resolution, steps for preparation/fixation -
CORRECT ANSWER -dead, 0.2 nm, not applicable, high resolution of internal cellular
structure, cost and cells dead
The cell - CORRECT ANSWER -basic unit of biology
Smooth ER - CORRECT ANSWER -Involved in the synthesis of lipids, and detoxification
of drugs and toxins and in storage of ions.
Rough ER - CORRECT ANSWER -That portion of the endoplasmic reticulum studded
with ribosomes.
, Electron Tomography - CORRECT ANSWER -a TEM method used to obtain high
resolution, 3D data of cell structure
Centrifugation - CORRECT ANSWER -procedure done to separate cellular components
basic on size, shape or density.
differential centrifugation - CORRECT ANSWER -uses higher speeds of centrifugation to
fractionate cell homogenates based on size and density
Density gradient Centrifugation - CORRECT ANSWER -fractionates cell homogenates in
a sucrose gradient based on buoyant density of homogenate
Electrophoresis - CORRECT ANSWER -a method used to separate isolated peptides
based on size and or charge when exposed to an electrical current.
Column Chromatography - CORRECT ANSWER -separates mixtures of proteins, nucleic
acids, and other molecules based on the fact that molecules dissolved in a solution will interact
with a solid surface.
Western Blotting - CORRECT ANSWER -used to identify peptides after separated by
electrophoresis methods
Flow Cytometry - CORRECT ANSWER -used to identify and separate cells by measuring
the light they scatter or the fluorescence they admit
Tensegrity - CORRECT ANSWER -interconnected system that stabilized itself
mechanically due to the way tensile and compressive forces are balanced
Mass Spectrometry - CORRECT ANSWER -a technique that separates particles according
to their mass
Exam Questions and CORRECT Answers
phase contrast microscope: state of cell, resolution, steps for preparation/fixation - CORRECT
ANSWER -Live, 0.2 nm, no fixation, live cell imaging, limited resoluton
Amplitude Contrast Microscope: state of cell, resolution, steps for preparation/fixation -
CORRECT ANSWER -Dead, 0.2 nm, Fix-dehydrate-embed in parafinn-section-stain, cells
are preserved and can be stored for reexamination, cell/tissues r dead
Fluorescence/Confocal Microscopy: state of cell, resolution, steps for preparation/fixation -
CORRECT ANSWER -Live (GFP) dead (immuno), 0.2nm, transform cells with florescent
protein gene OR fix cells for indirect immuno stain, image specific macromolecules and
orgenelles, cost
Transmission Electron Microscope (TEM): state of cell, resolution, steps for preparation/fixation
- CORRECT ANSWER -dead, 0.2nm, Fix-dehydrate-embed in parafinn-section-stain,
high resolution of internal cellular structure, cost and cells are dead and challenging
Scanning Electron Microscope (SEM): state of cell, resolution, steps for preparation/fixation -
CORRECT ANSWER -dead, 0.2 nm, not applicable, high resolution of internal cellular
structure, cost and cells dead
The cell - CORRECT ANSWER -basic unit of biology
Smooth ER - CORRECT ANSWER -Involved in the synthesis of lipids, and detoxification
of drugs and toxins and in storage of ions.
Rough ER - CORRECT ANSWER -That portion of the endoplasmic reticulum studded
with ribosomes.
, Electron Tomography - CORRECT ANSWER -a TEM method used to obtain high
resolution, 3D data of cell structure
Centrifugation - CORRECT ANSWER -procedure done to separate cellular components
basic on size, shape or density.
differential centrifugation - CORRECT ANSWER -uses higher speeds of centrifugation to
fractionate cell homogenates based on size and density
Density gradient Centrifugation - CORRECT ANSWER -fractionates cell homogenates in
a sucrose gradient based on buoyant density of homogenate
Electrophoresis - CORRECT ANSWER -a method used to separate isolated peptides
based on size and or charge when exposed to an electrical current.
Column Chromatography - CORRECT ANSWER -separates mixtures of proteins, nucleic
acids, and other molecules based on the fact that molecules dissolved in a solution will interact
with a solid surface.
Western Blotting - CORRECT ANSWER -used to identify peptides after separated by
electrophoresis methods
Flow Cytometry - CORRECT ANSWER -used to identify and separate cells by measuring
the light they scatter or the fluorescence they admit
Tensegrity - CORRECT ANSWER -interconnected system that stabilized itself
mechanically due to the way tensile and compressive forces are balanced
Mass Spectrometry - CORRECT ANSWER -a technique that separates particles according
to their mass
