AEST Standard 24- cell tissue-cell culture techniques Questions and Answers Already Passed
What is often the major source of explants for tissue culture? - Answersthe meristematic ends
of the plants like the stem tip or axillary bud
What are the major benefits of plant tissue culture? - AnswersThe ability to quickly produce
mature plants, produce multiples of plants in the absence of seeds or pollinators, produce
copies of plants with desirable traits, produce plants that otherwise have a low chance of
germinating, mass propagate plants for commercial use, and produce disease free plants.
Why are plant hormones often added to growth media in tissue culture? - AnswersTo increase
plant growth
What can be used to sterilize the air and exposed work surfaces in a cell culture hood? -
AnswersUV light
Which direction should your covers or lids face when setting them down on the work surface? -
AnswersDown, to prevent airborne contaminants from landing on the inside of them
How should you sterilize your hands and work surface? - AnswersBy wiping them down with
70% alcohol
What is the purpose of a laminar airflow hood in a tissue culture facility? - AnswersTo protect
the tissue culture from contamination by foreign particles and bacteria
How does a laminar airflow hood protect cell cultures from contamination? - AnswersBy
expelling filtered air through the hood and out the front, preventing airborne contamination from
entering the hood
Once plant tissues have been prepared for tissue culture, where is the tissue grown? -
Answersin sterile containers, such as petri dishes or flasks, in a room with controlled
temperature and light intensity
What is an explant? - Answersa sample of plant tissue used for tissue culture
What does the hormone auxin cause in plant tissue cultures? - AnswersIt causes the explant to
grow root tissues
What does the hormone cytokinin cause in plant tissue cultures? - AnswersIt may cause the
explant to grow shoots
What does a balance of cytokinin and auxin cause in plant tissue cultures? - AnswersIt often
results in an unorganised growth of cells
What is the unorganised growth of cells that is produced by an explant being grown in the
presence of both auxin and cytokinin called? - AnswersA callus
What is often the major source of explants for tissue culture? - Answersthe meristematic ends
of the plants like the stem tip or axillary bud
What are the major benefits of plant tissue culture? - AnswersThe ability to quickly produce
mature plants, produce multiples of plants in the absence of seeds or pollinators, produce
copies of plants with desirable traits, produce plants that otherwise have a low chance of
germinating, mass propagate plants for commercial use, and produce disease free plants.
Why are plant hormones often added to growth media in tissue culture? - AnswersTo increase
plant growth
What can be used to sterilize the air and exposed work surfaces in a cell culture hood? -
AnswersUV light
Which direction should your covers or lids face when setting them down on the work surface? -
AnswersDown, to prevent airborne contaminants from landing on the inside of them
How should you sterilize your hands and work surface? - AnswersBy wiping them down with
70% alcohol
What is the purpose of a laminar airflow hood in a tissue culture facility? - AnswersTo protect
the tissue culture from contamination by foreign particles and bacteria
How does a laminar airflow hood protect cell cultures from contamination? - AnswersBy
expelling filtered air through the hood and out the front, preventing airborne contamination from
entering the hood
Once plant tissues have been prepared for tissue culture, where is the tissue grown? -
Answersin sterile containers, such as petri dishes or flasks, in a room with controlled
temperature and light intensity
What is an explant? - Answersa sample of plant tissue used for tissue culture
What does the hormone auxin cause in plant tissue cultures? - AnswersIt causes the explant to
grow root tissues
What does the hormone cytokinin cause in plant tissue cultures? - AnswersIt may cause the
explant to grow shoots
What does a balance of cytokinin and auxin cause in plant tissue cultures? - AnswersIt often
results in an unorganised growth of cells
What is the unorganised growth of cells that is produced by an explant being grown in the
presence of both auxin and cytokinin called? - AnswersA callus
