BCHM 4360 Questions and Verified Answers,
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HB: What is the main question being addressed? - Correct
Answers ✅Is there regularity to the positioning of the histones
in DNA?
HB: What is the experimental approach for Figure 1? - Correct
Answers ✅-rat liver nuclei were incubated in the presence of
CaCl2 and MgCl2 for 0, 20, 40, 60, and 80 min
-performed polyacrylamide gel electrophoresis of rat liver nuclear
DNA that was digested by the Ca-Mg endonuclease
HB: What does the data from Figure 1 indicate? - Correct Answers
✅-Ca-Mg endonuclease is activated by calcium and magnesium
ions
-the longer time spent incubating in the presence of CaCl2 and
MgCl2, the more DNA in the bands representing the lower
molecular weight species
-reached a limit pattern quickly (fragments could not get smaller
than a certain size even with longer incubation)
-bands are regularly spaced
HB: What is the experimental approach for Figure 2? - Correct
Answers ✅-distance of the band from origin of the gel was
plotted on the y axis (log scale)
, BCHM 4360 Questions and Verified Answers,
100% Score Guarantee Pass (Latest 2025)
-square root of the mobility of the bands were plotted on the x
axis
HB: What does the data from Figure 2 indicate? - Correct Answers
✅-graph is a linear line
-their molecular weights are multiples of the molecular weight of
the smallest species
HB: What is the experimental approach for Figure 3? - Correct
Answers ✅-Ca-Mg endonuclease was extracted from rat liver
nuclei using KCl, K-phosphate, ethylene glycol and dialysing into
tris, MgCl2, ethylene glycol, 2-mercaptoethanol, and HCl (crude
enzyme)
-extract was incubated with calf thymus DNA in the presence of
CaCl2 for 0, 45, 90, and 120 min
-performed polyacrylamide gel electrophoresis of calf thymus
DNA that was digested by the Ca-Mg endonuclease
HB: What does the data from Figure 3 indicate? - Correct Answers
✅-continuous range of molecular weights
-no discrete bands observed
-DNA did not have proteins (histones) to protect it
100% Score Guarantee Pass (Latest 2025)
HB: What is the main question being addressed? - Correct
Answers ✅Is there regularity to the positioning of the histones
in DNA?
HB: What is the experimental approach for Figure 1? - Correct
Answers ✅-rat liver nuclei were incubated in the presence of
CaCl2 and MgCl2 for 0, 20, 40, 60, and 80 min
-performed polyacrylamide gel electrophoresis of rat liver nuclear
DNA that was digested by the Ca-Mg endonuclease
HB: What does the data from Figure 1 indicate? - Correct Answers
✅-Ca-Mg endonuclease is activated by calcium and magnesium
ions
-the longer time spent incubating in the presence of CaCl2 and
MgCl2, the more DNA in the bands representing the lower
molecular weight species
-reached a limit pattern quickly (fragments could not get smaller
than a certain size even with longer incubation)
-bands are regularly spaced
HB: What is the experimental approach for Figure 2? - Correct
Answers ✅-distance of the band from origin of the gel was
plotted on the y axis (log scale)
, BCHM 4360 Questions and Verified Answers,
100% Score Guarantee Pass (Latest 2025)
-square root of the mobility of the bands were plotted on the x
axis
HB: What does the data from Figure 2 indicate? - Correct Answers
✅-graph is a linear line
-their molecular weights are multiples of the molecular weight of
the smallest species
HB: What is the experimental approach for Figure 3? - Correct
Answers ✅-Ca-Mg endonuclease was extracted from rat liver
nuclei using KCl, K-phosphate, ethylene glycol and dialysing into
tris, MgCl2, ethylene glycol, 2-mercaptoethanol, and HCl (crude
enzyme)
-extract was incubated with calf thymus DNA in the presence of
CaCl2 for 0, 45, 90, and 120 min
-performed polyacrylamide gel electrophoresis of calf thymus
DNA that was digested by the Ca-Mg endonuclease
HB: What does the data from Figure 3 indicate? - Correct Answers
✅-continuous range of molecular weights
-no discrete bands observed
-DNA did not have proteins (histones) to protect it
