Enterobacteriaceae Exam Questions With Complete ... bb bb bb bb bb
Biochemically, the Enterobacteriaceae are gram-negative rods that: bb bb bb bb bb bb
A. Ferment glucose, reduce nitrate to nitrite, and are oxidase negative
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B. Ferment glucose, produce indophenol oxidase, and form gas
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C. Ferment lactose and reduce nitrite to nitrogen gas
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D. Ferment lactose and produce indophenol oxidase - ANS>>A. Ferment glucose,
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reduce nitrate to nitrite, and
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are oxidase negative
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The ortho-nitrophenyl-β-galactopyranoside (ONPG) test is most useful when
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differentiating:
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A. Salmonella spp. from Pseudomonas spp.
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B. Shigella spp. from some strains of Escherichia coli
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C. Klebsiella spp. from Enterobacter spp.
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D. Proteus vulgaris from Salmonella spp. - ANS>>B. Shigella spp. from some strains
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of Escherichia coli
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The Voges-Proskauer (VP) test detects which end product of glucose fermentation?
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A. Acetoinbb
B. Nitritebb
C. Acetic acid
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D. Hydrogen sulfide - ANS>>A. Acetoin
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At which pH does the methyl red (MR) test become positive?
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A. 7.0 bb
B. 6.5 bb
C. 6.0 bb bb
D. 4.5 - ANS>>D. 4.5
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A positive Simmons citrate test is seen as a:
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A. Blue color in the medium after 24 hours of incubation at 35°C
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B. Red color in the medium after 18 hours of incubation at 35°C
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C. Yellow color in the medium after 24 hours of incubation at 35°C
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D. Green color in the medium after 18 hours of incubation at 35°C - ANS>>A. Blue
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color in the medium after 24 hours of incubation at 35°C
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, In the test for urease production, ammonia reacts to form which product?
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A. Ammonium citrate
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B. Ammonium carbonate
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C. Ammonium oxalate
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D. Ammonium nitrate - ANS>>B. Ammonium carbonate
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Which of the following reagents is added to detect the production of indole?
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A. p-Dimethylaminobenzaldehyde
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B. Bromcresol purple
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C. Methyl red
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D. Cytochrome oxidase - ANS>>A. p-Dimethylaminobenzaldehyde
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Decarboxylation of the amino acids lysine, ornithine, and arginine results in the
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formation of:
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A. Ammonia
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B. Urea
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C. CO2bb
D. Amines - ANS>>D. Amines
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Lysine iron agar (LIA) showing a purple slant and a blackened butt indicates:
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A. E. coli
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B. Citrobacter spp.
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C. Salmonella spp.
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D. Proteus spp. - ANS>>C. Salmonella spp.
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Putrescine is an alkaline amine product of which bacterial enzyme?
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A. Arginine decarboxylase
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B. Phenylalanine deaminase
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C. Ornithine decarboxylase
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D. Lysine decarboxylase - ANS>>C. Ornithine decarboxylase
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Which genera are positive for phenylalanine deaminase?
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A. Enterobacter, Escherichia, and Salmonella
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B. Morganella, Providencia, and Proteus
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C. Klebsiella and Enterobacter
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D. Proteus, Escherichia, and Shigella - ANS>>B. Morganella, Providencia, and
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Proteus
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Kligler iron agar (KIA) differs from triple-sugar iron agar (TSI) in the:
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Biochemically, the Enterobacteriaceae are gram-negative rods that: bb bb bb bb bb bb
A. Ferment glucose, reduce nitrate to nitrite, and are oxidase negative
bb bb bb bb bb bb bb bb bb bb
B. Ferment glucose, produce indophenol oxidase, and form gas
bb bb bb bb bb bb bb bb
C. Ferment lactose and reduce nitrite to nitrogen gas
bb bb bb bb bb bb bb bb
D. Ferment lactose and produce indophenol oxidase - ANS>>A. Ferment glucose,
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reduce nitrate to nitrite, and
bb bb bb bb bb
are oxidase negative
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The ortho-nitrophenyl-β-galactopyranoside (ONPG) test is most useful when
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differentiating:
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A. Salmonella spp. from Pseudomonas spp.
bb bb bb bb bb
B. Shigella spp. from some strains of Escherichia coli
bb bb bb bb bb bb bb bb bb
C. Klebsiella spp. from Enterobacter spp.
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D. Proteus vulgaris from Salmonella spp. - ANS>>B. Shigella spp. from some strains
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of Escherichia coli
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The Voges-Proskauer (VP) test detects which end product of glucose fermentation?
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A. Acetoinbb
B. Nitritebb
C. Acetic acid
bb bb
D. Hydrogen sulfide - ANS>>A. Acetoin
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At which pH does the methyl red (MR) test become positive?
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A. 7.0 bb
B. 6.5 bb
C. 6.0 bb bb
D. 4.5 - ANS>>D. 4.5
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A positive Simmons citrate test is seen as a:
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A. Blue color in the medium after 24 hours of incubation at 35°C
bb bb bb bb bb bb bb bb bb bb bb bb
B. Red color in the medium after 18 hours of incubation at 35°C
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C. Yellow color in the medium after 24 hours of incubation at 35°C
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D. Green color in the medium after 18 hours of incubation at 35°C - ANS>>A. Blue
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color in the medium after 24 hours of incubation at 35°C
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, In the test for urease production, ammonia reacts to form which product?
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A. Ammonium citrate
bb bb
B. Ammonium carbonate
bb bb
C. Ammonium oxalate
bb bb bb
D. Ammonium nitrate - ANS>>B. Ammonium carbonate
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Which of the following reagents is added to detect the production of indole?
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A. p-Dimethylaminobenzaldehyde
bb
B. Bromcresol purple
bb bb
C. Methyl red
bb bb
D. Cytochrome oxidase - ANS>>A. p-Dimethylaminobenzaldehyde
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Decarboxylation of the amino acids lysine, ornithine, and arginine results in the
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formation of:
bb bb
A. Ammonia
bb
B. Urea
bb bb
C. CO2bb
D. Amines - ANS>>D. Amines
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Lysine iron agar (LIA) showing a purple slant and a blackened butt indicates:
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A. E. coli
bb bb
B. Citrobacter spp.
bb bb
C. Salmonella spp.
bb bb bb
D. Proteus spp. - ANS>>C. Salmonella spp.
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Putrescine is an alkaline amine product of which bacterial enzyme?
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A. Arginine decarboxylase
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B. Phenylalanine deaminase
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C. Ornithine decarboxylase
bb bb bb
D. Lysine decarboxylase - ANS>>C. Ornithine decarboxylase
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Which genera are positive for phenylalanine deaminase?
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A. Enterobacter, Escherichia, and Salmonella
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B. Morganella, Providencia, and Proteus
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C. Klebsiella and Enterobacter
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D. Proteus, Escherichia, and Shigella - ANS>>B. Morganella, Providencia, and
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Proteus
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Kligler iron agar (KIA) differs from triple-sugar iron agar (TSI) in the:
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