HOLECHEK BIO 340 EXAM 3 QUESTIONS AND
ANSWERS TOP SCORE 2025.
1. PCR: This technique uses an extremophile in order to replicate DNA quickly.
2. denaturation: The first thing that happens to DNA during the PCR process is
?
3. primer annealing: The second thing that happens during the PCR process is
?
4. primer annealing: phase in PCR during which a primer binds to a template strand
5. extension: The final step of the PCR process is termed ?
6. negative: what type of electric charge does DNA have?
7. size: Other than charge what is a crucial component of DNA during gel elec-
trophoresis?
8. Probes: Short DNA fragments that can be stained with something such as a
radioactive dye in order to further understand DNA are called
9. Conjugation: Transfer of replicated DNA from a donor to a recipient
10. Conjugation: In bacteria, the direct transfer of DNA between two cells that are
temporarily joined.
11. Pilus: What important structure is used to transfer replicated DNA during con-
jugation
12. enviroment: Where does the DNA come from during transformation?
13. Plasmid: Transformation can be done with DNA fragments OR a DNA
14. Transduction: DNA is transferred from a donor cell to a recipient via a bacte-
riophage
15. Transduction: Transfer of DNA from one bacterium to another using a viral
vector is called
16. antibiotic resistance: Sometimes plasmids can cause problems when they
contain harmful information because when treating the patient its found these
plasmids have ( two words)
17. multiple cloning site: region in plasmid that has been engineered to contain
recognition sites of a number of restriction endonucleases
18. Lac Z: The most common reporter gene used in plasmids is the _ operon
19. white: During white/blue screening which of the two contains the recombinant
plasmids?
20. The matrix: Bacteriophages look like a creepy creature out of what film series
featuring keanu reeves?
21. palindromic: reading DNA ends up being the same backwards and forwards
1 g/ g
5
, earns the DNA the descriptive term
22. Ligase: The enzyme that is crucial for piecing DNA back together is called
23. sticky: Which is more effecient in terms of being pieced back together, blunt
ends or sticky ends?(one word answer)
2 g/ g
5
ANSWERS TOP SCORE 2025.
1. PCR: This technique uses an extremophile in order to replicate DNA quickly.
2. denaturation: The first thing that happens to DNA during the PCR process is
?
3. primer annealing: The second thing that happens during the PCR process is
?
4. primer annealing: phase in PCR during which a primer binds to a template strand
5. extension: The final step of the PCR process is termed ?
6. negative: what type of electric charge does DNA have?
7. size: Other than charge what is a crucial component of DNA during gel elec-
trophoresis?
8. Probes: Short DNA fragments that can be stained with something such as a
radioactive dye in order to further understand DNA are called
9. Conjugation: Transfer of replicated DNA from a donor to a recipient
10. Conjugation: In bacteria, the direct transfer of DNA between two cells that are
temporarily joined.
11. Pilus: What important structure is used to transfer replicated DNA during con-
jugation
12. enviroment: Where does the DNA come from during transformation?
13. Plasmid: Transformation can be done with DNA fragments OR a DNA
14. Transduction: DNA is transferred from a donor cell to a recipient via a bacte-
riophage
15. Transduction: Transfer of DNA from one bacterium to another using a viral
vector is called
16. antibiotic resistance: Sometimes plasmids can cause problems when they
contain harmful information because when treating the patient its found these
plasmids have ( two words)
17. multiple cloning site: region in plasmid that has been engineered to contain
recognition sites of a number of restriction endonucleases
18. Lac Z: The most common reporter gene used in plasmids is the _ operon
19. white: During white/blue screening which of the two contains the recombinant
plasmids?
20. The matrix: Bacteriophages look like a creepy creature out of what film series
featuring keanu reeves?
21. palindromic: reading DNA ends up being the same backwards and forwards
1 g/ g
5
, earns the DNA the descriptive term
22. Ligase: The enzyme that is crucial for piecing DNA back together is called
23. sticky: Which is more effecient in terms of being pieced back together, blunt
ends or sticky ends?(one word answer)
2 g/ g
5
