MICROBIOLOGY LAB PRACTICAL – FINAL EXAM QUESTIONS AND CORRECT ANSWERS 2025/2026

MICROBIOLOGY LAB PRACTICAL – FINAL EXAM QUESTIONS AND CORRECT ANSWERS 2025/2026 Staph epidermidis - Answer -This is MSA and the side labelled "2" is red. Which Staph does this indicate? Staph aureus - Answer -This is MSA and the side labelled "1" has turned yellow. Which Staph does this indicate? Lactose Negative - Answer -This is an EMB plate. What does the test result of "1" indicate? Lactose Positive - Answer -This is an EMB plate. What does the test result of "2" indicate? E. Coli - Answer -This is an EMB plate. What is indicated by "3?" Starch was broken down - Answer -This is a starch plate. What does the clear boundary around this colony indicate? acid was produced - Answer -Why did this Fermentation Tube turn yellow? gas was produced - Answer -What is indicated by the bubble in this Durham Tube inside a Fermentation Tube? Protein was not broken down - Answer -This is a Gelatin Tube Test. In Test Tube 2, it is solid. What does this mean?Catalase Test - Answer -What is the name of this test? Antibiotic Disk Diffusion (Antibiotic Susceptibility) - Answer -What is the name of this test? Mannitol Salt Agar - Answer -What is the name of this media? Urea Broth Urease Enzyme Tube 1: Positive Tube 2: Negative - Answer -What is it testing for? What does pink indicate? What does the pale yellow indicate? Nitrate Reduction - Answer -What is the name of this test? neutrophil - Answer - lymphocyte - Answer - monocyte - Answer - eosinophil - Answer - basophil - Answer - What method is used for the antimicrobial susceptibility test? - Answer -Kirby-Bauer What technique is used for the Kirby-Bauer test? - Answer -swab and make a bacterial lawnWhat is the principle of technique for the Kirby-Bauer? - Answer -disk diffusion What observations are made in lab with Kirby-Bauer? - Answer -zone of inhibition What is reported out to Physicians from the Kirby-Bauer? - Answer - susceptibility or resistance What is produced when UV radiation is absorbs by DNA? - Answer - pyrimidine dimers - which interferes with DNA replication and transcription