Hematology Clinical Laboratory Science Methodology Exam with Q and A

Hematology Clinical Laboratory Science Methodology Exam with Q and A What combination of reagents is used to measure hemoglobin? A. Hydrochloric acidHct (%) and p-dimethylaminobenzaldehyde B. Potassium ferricyanide and potassium cyanide C. Sodium bisulfite and sodium metabisulfite D. Sodium citrate and hydrogen peroxide ANSWER B. Potassium ferricyanide and potassium cyanide B. The standard assay for hemoglobin utilizes potassium ferricyanide. This solution, formerly called Drabkin's reagent, is now called cyanmethemoglobin (HiCN) reagent. The ferricyanide oxidizes hemoglobin iron from ferrous (Fe2+) to ferric (Fe3+), and the potassium cyanide stabilizes the pigment as cyanmethemoglobin for spectrophotometric measurement The slowest-moving hemoglobin(s) on an alkaline electrophoresis at pH 8.6 is(are) A. A B. A2, C,E, and O C. F D. S, D, andG ANSWER B. A2, C,E, and O