HLA CHT PRACTICE EXAM QUESTIONS
WITH COMPLETE SOLUTIONS
Blood specimens known to carry pathogens are packaged the same as routine
specimens..... true or false - ANSWER-True
When performing a blood draw, what is not part of the routine procedure?
Change gloves in between patients
Informing patient of HIV status
Wear a lab coat or gown - ANSWER-Informing patient of HIV status
What is a fire assembly area? - ANSWER-A safe place to meet during a fire, that is
predetermined by employer
SSP and SSOP reagents commonly target specific regions of the HLA Class I genes,
the regions are what?
A) Introns
B) Conserved Regions
C) Polymorphic Regions
D) Promoter Regions - ANSWER-C....Polymorphic Regions
Which Acronym is wrong?
A) ASHI- American Society for Histocompatibility and Immunogenetics
B) PCR- Polymerase Chain Reaction
C) CLIA- Clinical Laboratory Improvement Amendments
D) NGS- Next Genomic Sequence - ANSWER-D......Next Genomic Sequence is
supposed to be Next Generation Sequence
What Probe would hybridize under stringent conditions to the following sequence-5'
AGTTCGATGC?
A) 5' GCATCGAACT
B) 3' AGTTCGATGC
C) 3' GCATCGAACT
D) 5' AGTTCGATGC - ANSWER-A.... GCATCGAACT
Reagent x arrives in the lab, What action is the best action to do? - ANSWER-
Periodically QC the reagent and develop a QA analysis of its performance to derive an
appropriate expiration date
, You have a patient that does not want to be listed. What should you do with all of the
reports, typing, paperwork etc? Some is online (LIS) and others are on paper. -
ANSWER-Keep said paperwork for two years as long as the LIS is backed up regularly
and paper storage is secure
A 100ul stock solution of DNA is 160 ug/ml. What final volume should you dilute the
stock, to achieve a working concentration of 40ug/ml? - ANSWER-400ul You would use
the formula C1V1=C2V2
100x160= 1600 1600/40=400 C
Solution three combines all of solution one and two. Solution one consists of 2.0ml of
cells at a concentration 5x10 to the 6th, Solution two consists of 7.0ml of cells at a
concentration of 3x10 to the 6th. What is the cell concentration of solution 3. -
ANSWER-3.4 x 10 to the 6th cells/ml
2.0x5=10
7.0x3=21
31cells/ml divided by total volume of solution one and two so 9mls=3.4cells/ml
What is equivalent to 2N H2SO4? The molecular weight of H2SO4 is 98g/mol -
ANSWER-9.8g H2SO4 om 100ml of H2O
100g of NaCl (NA=22.99g/mol Cl=35.43g/mol) is dissolved in 1500ml of water, what is
the molarity? - ANSWER-1.14m
Approximately ho many grams of sodium chloride are required per 100ml of water? -
ANSWER-0.85
Would you be able to use blood that was collected in EDTA for serology? - ANSWER-
No
What organ yields the most B cells? - ANSWER-Spleen
How should whole blood specimens drawn into ACD tubes be stored? - ANSWER-
Room temperature
The absolute A260 and A280 values and their ratio for a DNA sample provide what
information?
a. Length and concentration
b. Free 3' ends and length
c. Concentration and RNA/Protein contamination
d. Concentration and percent degraded - ANSWER-Concentration and RNA/Protein
contamination
WITH COMPLETE SOLUTIONS
Blood specimens known to carry pathogens are packaged the same as routine
specimens..... true or false - ANSWER-True
When performing a blood draw, what is not part of the routine procedure?
Change gloves in between patients
Informing patient of HIV status
Wear a lab coat or gown - ANSWER-Informing patient of HIV status
What is a fire assembly area? - ANSWER-A safe place to meet during a fire, that is
predetermined by employer
SSP and SSOP reagents commonly target specific regions of the HLA Class I genes,
the regions are what?
A) Introns
B) Conserved Regions
C) Polymorphic Regions
D) Promoter Regions - ANSWER-C....Polymorphic Regions
Which Acronym is wrong?
A) ASHI- American Society for Histocompatibility and Immunogenetics
B) PCR- Polymerase Chain Reaction
C) CLIA- Clinical Laboratory Improvement Amendments
D) NGS- Next Genomic Sequence - ANSWER-D......Next Genomic Sequence is
supposed to be Next Generation Sequence
What Probe would hybridize under stringent conditions to the following sequence-5'
AGTTCGATGC?
A) 5' GCATCGAACT
B) 3' AGTTCGATGC
C) 3' GCATCGAACT
D) 5' AGTTCGATGC - ANSWER-A.... GCATCGAACT
Reagent x arrives in the lab, What action is the best action to do? - ANSWER-
Periodically QC the reagent and develop a QA analysis of its performance to derive an
appropriate expiration date
, You have a patient that does not want to be listed. What should you do with all of the
reports, typing, paperwork etc? Some is online (LIS) and others are on paper. -
ANSWER-Keep said paperwork for two years as long as the LIS is backed up regularly
and paper storage is secure
A 100ul stock solution of DNA is 160 ug/ml. What final volume should you dilute the
stock, to achieve a working concentration of 40ug/ml? - ANSWER-400ul You would use
the formula C1V1=C2V2
100x160= 1600 1600/40=400 C
Solution three combines all of solution one and two. Solution one consists of 2.0ml of
cells at a concentration 5x10 to the 6th, Solution two consists of 7.0ml of cells at a
concentration of 3x10 to the 6th. What is the cell concentration of solution 3. -
ANSWER-3.4 x 10 to the 6th cells/ml
2.0x5=10
7.0x3=21
31cells/ml divided by total volume of solution one and two so 9mls=3.4cells/ml
What is equivalent to 2N H2SO4? The molecular weight of H2SO4 is 98g/mol -
ANSWER-9.8g H2SO4 om 100ml of H2O
100g of NaCl (NA=22.99g/mol Cl=35.43g/mol) is dissolved in 1500ml of water, what is
the molarity? - ANSWER-1.14m
Approximately ho many grams of sodium chloride are required per 100ml of water? -
ANSWER-0.85
Would you be able to use blood that was collected in EDTA for serology? - ANSWER-
No
What organ yields the most B cells? - ANSWER-Spleen
How should whole blood specimens drawn into ACD tubes be stored? - ANSWER-
Room temperature
The absolute A260 and A280 values and their ratio for a DNA sample provide what
information?
a. Length and concentration
b. Free 3' ends and length
c. Concentration and RNA/Protein contamination
d. Concentration and percent degraded - ANSWER-Concentration and RNA/Protein
contamination
