FC assignments in de les

FC assignments;
Nummer; 1
Homo sapiens taste 2 receptor member 1 (TAS2R1), transcript variant 1, mRNA
Homo sapiens taste 2 receptor member 1 (TAS2R1), transcript variant 1, - Nucleotide - NCBI (nih.gov)

translation="MLESHLIIYFLLAVIQFLLGIFTNGIIVVVNGIDLIKHRKMAPL

DLLLSCLAVSRIFLQLFIFYVNVIVIFFIEFIMCSANCAILLFINELELWLATWLGVF

YCAKVASVRHPLFIWLKMRISKLVPWMILGSLLYVSMICVFHSKYAGFMVPYFLRKFF

SQNATIQKEDTLAIQIFSFVAEFSVPLLIFLFAVLLLIFSLGRHTRQMRNTVAGSRVP

GRGAPISALLSILSFLILYFSHCMIKVFLSSLKFHIRRFIFLFFILVIGIYPSGHSLI
LILGNPKLKQNAKKFLLHSKCCQ"




NCBI Blast:Protein Sequence (nih.gov)




Best match = gorilla gorilla gorilla

, ProtScale analysis (expasy.org)




➔ 6 trans memraan helices ipv 7? Twee helices samengeperst?




Forward primer: CCTTCGTTTTCTTGGTGAATTTTTGGGATGTAGTGAAGAGGCGG
Reverse primer; GGTTGGCTTGGTTTGCAATCATCC

Best match;
Homo sapiens taste 2 receptor member 38 (TAS2R38), mRNA
https://www.ncbi.nlm.nih.gov/nuccore/NM_176817.5


Match forward primer;