PCR AND PRIMER APPLICATION DESIGNING BIOCHEMICAL TECHNIQUES DR. WAJEEHA SAEED PH.D.

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PCR AND PRIMER
APPLICATION DESIGNING
BIOCHEMICAL TECHNIQUES
DR. WAJEEHA SAEED PH.D.

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PCR - Polymerase Chain Reaction
• PCR is an in vitro technique for the amplification of a region of DNA which lies
between two regions of known sequence.
• PCR amplification is achieved by using oligonucleotide primers.
• These are typically short, single stranded oligonucleotides which are
complementary to the outer regions of known sequence.




• The oligonucleotides serve as primers for DNA polymerase and the denatured
strands of the large DNA fragment serves as the template.
• This results in the synthesis of new DNA strands which are complementary
to the parent template strands.

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• These new strands have defined 5' ends (the 5' ends of the oligonucleotide
primers), whereas the 3' ends are potentially ambiguous in length.

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In a crime scene, a sample of DNA was found, however amount of DNA was not
enough to
be analyzed.
▪▪ After DNA extraction, the scientist want to study a specific part of a gene to
do sequencing.